Phytochemical Profile, TLC Profiling and Antioxidant Potential of Prunus Avium
Abstract
This work looks at the Chromatographic content of the hydro-alcoholic extract of Prunus avium leaf and its antioxidant activity. The presence of essential metabolites was confirmed by preliminary phytochemical screening for glycosides, flavonoids, alkaloids, steroids, volatile oils, and tannins, indicating the plant's ethnomedicinal value. To maximise the separation and visibility of constituent substances, TLC was done using three solvent systems of varied polarity. Under 254 nm and 365 nm UV light, distinct, well-resolved bands were observed, indicating a different range of polar and mid-polar phytochemicals. Further antioxidant activity was assessed using DPPH and ABTS radical scavenging assays. The extensive chromatographic and phytochemical profiles obtained in the study highlight the potential of P. avium as a valid phytotherapeutic candidate, setting the stage for future bioactive-guided reclusion and anticancer testing.
Keywords: Prunus avium, sweet cherry, Chromatographic content, TLC analysis, hydroalcoholic extract.
Keywords:
Prunus avium, sweet cherry, Chromatographic content, TLC analysis, hydroalcoholic extractDOI
https://doi.org/10.22270/jddt.v16i1.7502References
1. Agrawal S, Kumar A, Singh AK, Singh H, Thareja S, Kumar P. A comprehensive review on pharmacognosy, phytochemistry and pharmacological activities of eight potent Prunus species of Southeast Asia. J Tradit Chin Med. 2024;44(3):620-628. https://doi.org/10.19852/j.cnki.jtcm.2024.03.002
2. Bento C, Goncalves AC, Silva B, Silva LR. Peach (Prunus persica): Phytochemicals and health benefits. Food Rev Int. 2022;38(10):1703-1734. https://doi.org/10.1080/87559129.2020.1837861
3. Chezanoglou E, Mourtzinos I, Goula AM. Sweet cherry and its by-products as sources of valuable phenolic compounds. Trends Food Sci Technol. 2024;145:104367. https://doi.org/10.1016/j.tifs.2024.104367
4. Gonçalves AC, Bento C, Silva B, Simões M, Silva LR. Nutrients, bioactive compounds and bioactivity: The health benefits of sweet cherries (Prunus avium L.). Curr Nutr Food Sci. 2019;15(3):208-227. https://doi.org/10.2174/1573401313666170925154707
5. Jesus F, Goncalves AC, Alves G, Silva LR. Health benefits of Prunus avium plant parts: An unexplored source rich in phenolic compounds. Food Rev Int. 2022;38(2):118-146. https://doi.org/10.1080/87559129.2020.1854781
6. Kashyap P, Riar CS, Jindal N. Polyphenol bio-accessibility and antioxidant activity of in vitro digested ultrasound-assisted Meghalayan cherry (Prunus nepalensis) pomace extract. Biomass Convers Biorefinery. 2023;13:14071-14085. https://doi.org/10.1007/s13399-021-02150-0
7. Nunes AR, Gonçalves AC, Falcão A, Alves G, Silva LR. Prunus avium L. (sweet cherry) by-products: A source of phenolic compounds with antioxidant and anti-hyperglycemic properties-A review. Appl Sci. 2021;11(17):8516. https://doi.org/10.3390/app11188516
8. Kumar A, Nirmal P, Kumar M, Jose A, Tomer V, Oz E, Proestos C, Zeng M, Elobeid T, Sneha K, Faith Oz. Major phytochemicals: Recent advances in health benefits and extraction methods. Molecules. 2023;28(3):887. https://doi.org/10.3390/molecules28020887 PMid:36677944 PMCid:PMC9862941
9. Amir M, Zafar A, Ahmad R, Ahmad W, Sarafroz M, Khalid M, Ghoneim MM, Alshehri S, Wahab S, Ahmad S. Quality control standardization, contaminant detection and in vitro antioxidant activity of Prunus domestica Linn. fruit. Plants. 2022;11(5):706. https://doi.org/10.3390/plants11050706 PMid:35270176 PMCid:PMC8912893
10. Móricz ÁM, Ott PG. Separation and detection of apricot leaf triterpenes by high-performance thin-layer chromatography combined with direct bioautography and mass spectrometry. J Chromatogr A. 2022;1675:463167. https://doi.org/10.1016/j.chroma.2022.463167 PMid:35635866
11. Ockun MA, Gercek YC, Demirsoy H, Demirsoy L, Macit I, Oz GC. Comparative evaluation of phenolic profile and antioxidant activity of new sweet cherry (Prunus avium L.) genotypes in Turkey. Phytochem Anal. 2022;33(4):564-576. https://doi.org/10.1002/pca.3110 PMid:35122339
12. Zarzycki PK, Ślączka MM, Zarzycka MB, Włodarczyk E, Baran MJ. Application of micro-thin-layer chromatography as a simple fractionation tool for fast screening of raw extracts derived from complex biological, pharmaceutical and environmental samples. Anal Chim Acta. 2011;688(2):168-174. https://doi.org/10.1016/j.aca.2011.01.001 PMid:21334482
13. Lapa-Guimarães J, Pickova J. New solvent systems for thin-layer chromatographic determination of nine biogenic amines in fish and squid. J Chromatogr A. 2004;1045(1-2):223-232. https://doi.org/10.1016/j.chroma.2004.06.014 PMid:15378899
14. Mahmoud SA, Abbas AEF. Greenness, whiteness, and blueness assessment with spider chart solvents evaluation of HPTLC-densitometric method for quantifying a triple combination anti-Helicobacter pylori therapy. Sustain Chem Pharm. 2024;37:101412. https://doi.org/10.1016/j.scp.2023.101412
15. Nenadis N, Tsimidou MZ. DPPH (2,2‐di(4‐tert‐octylphenyl)‐1‐picrylhydrazyl) radical scavenging mixed‐mode colorimetric assay(s). Meas Antioxid Act Capacit Recent Trends Appl. 2018;141-164. https://doi.org/10.1002/9781119135388.ch8
16. Sanna D, Delogu G, Mulas M, Schirra M, Fadda A. Determination of free radical scavenging activity of plant extracts through DPPH assay: An EPR and UV-Vis study. Food Anal Methods. 2012;5(4):759-766. https://doi.org/10.1007/s12161-011-9306-1
17. Zheng L, Zhao M, Xiao C, Zhao Q, Su G. Practical problems when using ABTS assay to assess the radical-scavenging activity of peptides: Importance of controlling reaction pH and time. Food Chem. 2016;192:288-294. https://doi.org/10.1016/j.foodchem.2015.07.015 PMid:26304349
18. Gulcin İ, Alwasel SH. DPPH radical scavenging assay. Processes. 2023;11(8):2248. https://doi.org/10.3390/pr11082248
19. Saeed RA, Khan MI, Butt MS, Faisal MN. Phytochemical screening of Prunus avium for its antioxidative and anti-mutagenic potential against DMBA-induced hepatocarcinogenesis. Front Nutr. 2023;10:1132356. https://doi.org/10.3389/fnut.2023.1132356 PMid:37266135 PMCid:PMC10231329
20. Usenik V, Fajt N, Mikulic-Petkovsek M, Slatnar A, Stampar F, Veberic R. Sweet cherry pomological and biochemical characteristics influenced by rootstock. J Agric Food Chem. 2010;58(8):4928-4933. https://doi.org/10.1021/jf903755b PMid:20337477
21. Pietta PG (2000). Flavonoids as antioxidants. Journal of Natural Products, 63(7), 1035-1042. https://doi.org/10.1021/np9904509 PMid:10924197
22. Rizvi S, Raza ST, & Ahmed F (2018). The role of plant-derived sterols and triterpenes in oxidative stress and inflammation: A review. Free Radical Research, 52(12), 1267-1281.
23. Saleh HA, Yousef MH, Abdelnaser A. The anti-inflammatory properties of phytochemicals and their effects on epigenetic mechanisms involved in TLR4/NF-κB-mediated inflammation. Front Immunol. 2021;12:606069. https://doi.org/10.3389/fimmu.2021.606069 PMid:33868227 PMCid:PMC8044831
24. Theansungnoen T, Nitthikan N, Wilai M, Chaiwut P, Kiattisin K, Intharuksa A. Phytochemical analysis and antioxidant, antimicrobial, and antiaging activities of ethanolic seed extracts of four Mucuna species. Cosmetics. 2022;9(1):14. https://doi.org/10.3390/cosmetics9010014
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