TLC Profiling and Phytochemical Evaluation of Kaempferia parviflora Rhizome: A Rich Source of Bioactive Metabolites

Authors

  • Swati Pandey Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India https://orcid.org/0009-0009-0989-7057
  • Shubhrat Maheshwari Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India https://orcid.org/0000-0002-2677-4229
  • Jagat Pal Yadav Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India https://orcid.org/0000-0001-8386-9825
  • Amita Verma Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India https://orcid.org/0000-0001-6653-7335

Abstract

The present study investigates the phytochemical composition and chromatographic profiling of the hydroalcoholic extract of Kaempferia parviflora rhizome. Preliminary phytochemical screening revealed the presence of key secondary metabolites including alkaloids, flavonoids, tannins, phenolics, saponins, steroids, glycosides, terpenoids, and volatile oils, supporting the plant's ethnomedicinal significance. Thin Layer Chromatography (TLC) analysis was performed using four solvent systems of varying polarity to optimize the separation and visualization of constituent compounds. Distinct and well-resolved bands were observed under UV light (254 nm and 365 nm), with Rf values ranging from 0.08 to 0.97, indicating a rich diversity of polar and moderately polar phytochemicals. Derivatization with vanillin-sulfuric acid further confirmed the presence of compound classes such as flavonoids, phenolics, terpenoids, and glycosides. The comprehensive phytochemical and chromatographic profiles obtained in this study underscore the potential of K. parviflora as a valuable phytopharmaceutical candidate, laying the groundwork for further bioactivity-guided isolation and anticancer assessment.

Keywords: Kaempferia parviflora, Black ginger, Phytochemical analysis, TLC analysis, hydroalcoholic extract.

Keywords:

Kaempferia parviflora, Black ginger, Phytochemical analysis, TLC analysis, hydroalcoholic extract

DOI

https://doi.org/10.22270/jddt.v15i6.7182

Author Biographies

Swati Pandey , Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

Shubhrat Maheshwari , Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

Jagat Pal Yadav , Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

Amita Verma , Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

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Published

2025-06-15
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How to Cite

1.
Pandey S, Maheshwari S, Yadav JP, Verma A. TLC Profiling and Phytochemical Evaluation of Kaempferia parviflora Rhizome: A Rich Source of Bioactive Metabolites. J. Drug Delivery Ther. [Internet]. 2025 Jun. 15 [cited 2026 Jan. 28];15(6):79-84. Available from: https://jddtonline.info/index.php/jddt/article/view/7182

How to Cite

1.
Pandey S, Maheshwari S, Yadav JP, Verma A. TLC Profiling and Phytochemical Evaluation of Kaempferia parviflora Rhizome: A Rich Source of Bioactive Metabolites. J. Drug Delivery Ther. [Internet]. 2025 Jun. 15 [cited 2026 Jan. 28];15(6):79-84. Available from: https://jddtonline.info/index.php/jddt/article/view/7182

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