Antibacterial Activity of Mirabilis jalapa Leaves (Karifuma) Extract Against Bacterial Wound Infection from Patients Attending Rango Health Center

  • NZABANTERURA Innocent Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda 
  • ISHIMWE ALAIN PRUDENCE Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda 
  • UWIZEYIMANA Jean Pierre Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda 
  • BIZIMANA Ezechiel Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda
  • UWIHANGANYE Jean Chrysostome Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda

Abstract

Background: Mirabilis jalapa is a perennial herbaceous bushy plant with several pharmacological activities, such as anti-diabetic, anti-inflammatory, anti-oxidative, anti-bacterial, anti-microbial, anti-fungal, anti-viral, diuretic and anthelmintic. Wound infections are a major public health concern in Rwanda, with 8% of the general population having difficulties controlling them and 25% of those above 60 years old. Traditional medicine is used to treat many conditions such as chest infections, coughs, snake-bites and burns. Mirabilis jalapa seeds have been reported to be effective against bacteria causing wound infections.  Aim: This study evaluated the antibacterial activity of Mirabilis jalapa leaves extract against infected wound bacteria from patients attending Rango Health Center.


Methods: Cross sectional study design and maceration method was used in Mirabilis jalapa leaves extraction with Methanol, Ethanol, Diethyl ether and Distilled water as solvents.


Results: Different extracts were obtained according to the solvents with different volumes and concentrations. The highest extract volume obtained was Distilled water with 6 ml and the least was Diethyl ether with 2.7 ml. In 71 participants who had wound infection, 42% had a bacterial infection. Among them, 25% had Staphylococcus aureus, 7% had Klebsiella pneumoniae, 6% had Escherichia coli, and 4% had Pseudomonas aeruginosa. Through the agar well diffusion method, Methanol extracts showed the highest inhibition zones while distilled water extracts exhibited the lowest inhibition zones to the isolated bacteria.


Conclusion: The study confirms the antibacterial activity of the Mirabilis jalapa leaves extract against infected wound bacteria, with methanol and ethanol being the best solvents.


Keywords: Mirabilis jalapa leaves, wound infections, antibacterial activity

Keywords: Mirabilis jalapa leaves, wound infections, antibacterial activity

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Author Biographies

NZABANTERURA Innocent, Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda 

University Teaching Hospital of Butare, Rwanda

ISHIMWE ALAIN PRUDENCE, Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda 

University of Rwanda, College of Medicine and Health Sciences, Department of Biomedical Laboratory Sciences, Rwanda

UWIZEYIMANA Jean Pierre, Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda 

King Faisal Hospital, Rwanda

BIZIMANA Ezechiel, Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda

Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda 

UWIHANGANYE Jean Chrysostome, Ines-Ruhengeri, Faculty of Applied Fundamental Sciences, Department of Biomedical Laboratory Sciences, Rwanda

Rwanda Military Hospital

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Innocent N, ALAIN PRUDENCE I, Jean Pierre U, Ezechiel B, Chrysostome U. Antibacterial Activity of Mirabilis jalapa Leaves (Karifuma) Extract Against Bacterial Wound Infection from Patients Attending Rango Health Center. JDDT [Internet]. 15Jun.2024 [cited 17Jul.2024];14(6):34-8. Available from: https://jddtonline.info/index.php/jddt/article/view/6633

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