Assessment of in vitro antioxidant and cytotoxic potentials of ethanol extract of Nymphaea nauchali
Objective: The present study was designed to evaluate ethanol extract of Nymphaea nauchali for anticancer against breast cancer cells and also to determine its antioxidant properties.
Methods: The ethanol extract of Nymphaea nauchali was prepared using sox-let apparatus. The ethanol extract was evaluated for its in-vitro anticancer potentials against human MCF-7 cancer cell lines by MTT assay and concentration of the ethanol extract required to inhibit 50% of cell growth (IC50) was recorded. To determine antioxidant properties of the extract, Superoxide scavenging, Lipid peroxidation and DPPH methods were used and IC50 values of extract was noted.
Results: In the present study, ethanol extract of Nymphaea nauchali has shown anticancer property by exhibiting significant IC50 values against MCF 7 cells. The extract has also shown significant IC50 values in Superoxide scavenging, Lipid peroxidation and DPPH methods and antioxidant activity was comparable to standard drug ascorbic acid.
Conclusion: The results obtained from the present research work suggested that the ethanol extract of Nymphaea nauchali possess significant in vitro cytotoxic potentials against breast cancer cells. The results also suggested that the ethanol extract have in vitro antioxidant properties.
Keywords: Anticancer activity, Nymphaea nauchali, IC50 value, MTT assay and antioxidant activity.
2. Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD, Dirks PB. Identification of human brain tumour initiating cells. Nature. 2004; 18; 432(7):396-401. https://doi.org/10.1038/nature03128
3. Abidemi JA, Zahoor W, Girish M, Sadhana S ,Flora RA, Naresh S, Olufunmilayo OA, Dilip MM. Anticancer activity of Aristolochia ringens Vahl. (Aristolochiaceae). J Trad Com Med 2015; 5: 35-41. https://doi.org/10.1016/j.jtcme.2014.05.001
4. Dhananjaya S and Aparna K. current status of cancer burden: Global and Indian scenario. Biomed Res J 2014; 1(1):1-5. https://doi.org/10.4103/2349-3666.240996
5. Nitesh K, Isha D, P. Vasanth R, B.S. Jayashree, Vipan P, S.N. Manjula, Seeja T, N. Gopalan K, C. Mallikarjuna R. Preliminary investigation of cytotoxic potentialof 2-quinolone derivatives using in vitro and in vivo (solid tumor and liquid tumor) models of cancer. Arabian J Chem 2014; 7:409-17. https://doi.org/10.1016/j.arabjc.2012.12.029
6. Sankappa RU, A.M. Isloor , P. Shetty , K.S.R. Pai , H.K. Fun. Synthesis and in vitro biological evaluation of newpyrazole chalcones and heterocyclic diamides as potential anticancer agents. Arabian J ChemVolume 2015; 8(3):317-21. https://doi.org/10.1016/j.arabjc.2014.01.018
7. Thirumal M, Kishore G, Prithika R, Das S, Nithya G. In vitro anticancer activity of Tecoma stans (L) ethanolic leaf extract on human breast cancer cell line (MCF-7). Int J Pharm Chem Biol Sci 2012; 2(4):488-93.
8. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39:44-84. https://doi.org/10.1016/j.biocel.2006.07.001
9. Aboul-Enein AM, Abu-Elalla F, Shalaby EA, El-Shemy HA. Traditional medicinal plants research in Egypt: studies of antioxidant and anticancer activities. J Med Plants Res 2012; 6(5):689-703.
10. Saldanha CJ, Singh BG, Leguminosae, In: Saldanha C.J. (Ed.) Flora of Karnataka Vol:I, Oxford and IBH, 1984, 495-499.
11. Mohan Maruga Raja MK, Neeraj Kumar S, and Mishra SH. A comprehensive review on Nymphaea stellata: A traditionally used bitter. J Adv Pharm Technol Res. 2010; 1(3):311-9. https://doi.org/10.4103/0110-5558.72424
12. Manjeet S and Alok PJ. A review on genus Nymphaea: multi-potential medicinal plant. Asian J Pharm Edu & Res 2017; 6(4):1-9.
13. Priyabrata P ,Sridhar S, Bainateya M and Sudhir KP. Anthelmintic and Preliminary Phytochemical Screening of Nymphaea nouchali Burm.f. Against Intestinal Helminthiasis. Res J Pharm & Technol 2009; 2(3):537-9.
14. Kokate CK. Practical Pharmacognosy. Vallabh Prakashan New Delhi: 1994; 4:110-1.
15. Khandelwal KR, Practical Pharmacognosy-techniques and experiments. Pune; Nirali Prakashan; 2000.
16. Kai M, Klaus HV, Sebastian L, Ralf H, Andreas R, Ulf-Peter H. Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis spectrophotometric measurements. Sensors 2007; 7:2080-95. https://doi.org/10.3390/s7102080
17. Rajbir S, Saroj A. In vitro evaluation of peroxyl radical scavenging capacity of water extract/fractions of Acacia nilotica. Willd. Ex Del African J Biotechnol 2009; 8(7):1270-2.
18. Rupachandra S and Sarada DVL. Anticancer activity of methanol extract of the seeds of Borreria hispida and Momordica dioica. J pharmacy res. 2013; 6: 565-8. https://doi.org/10.1016/j.jopr.2013.04.027
19. Mc Govern et al. Anticancer activity of botanical compounds in ancient fermented beverages (Review). International J Oncology 2010; 37:5-14. https://doi.org/10.3892/ijo_00000647
20. Shankar S, Ganapathy S, Hingorani SR, Srivastava RK. EGCGGinhibitsGgrowth,Ginvasion, angiogenesisSand metastasis of pancreaticCcancer. Frontiers in Biosci 2008; 13(2):440-52. https://doi.org/10.2741/2691
21. Mc Govern et al. Anticancer activity of botanical compounds in ancient fermented beverages (Review). International J Oncology 2010; 37:5-14. https://doi.org/10.3892/ijo_00000647
22. Shankar S, Ganapathy S, Hingorani SR, Srivastava RK. EGCGGinhibitsGgrowth,Ginvasion, angiogenesisSand metastasis of pancreaticCcancer. Frontiers in Biosci 2008; 13(2):440-52. https://doi.org/10.2741/2691
23. Olinski R, Gackowski D, Foksinski M, Rozalski R, Roszkowski K, Jaruga P. Oxidative DNA damage: assessment of the role in carcinogenesis, atherosclerosis and acquired immunodeficiency syndrome. Free Radical Bio Med 2002; 33(9):192-200. https://doi.org/10.1016/S0891-5849(02)00878-X
24. Dhananjaya S and Aparna K. Current status of Cancer burden: Global and Indian scenario. Biomed Res J 2014; 1(1):1-5. https://doi.org/10.4103/2349-3666.240996
25. Ravindranath MH, Ramasamy V, Moon S, Ruiz C , Muthugounder S. DifferentialLgrowth suppressionNof human melanomAcells by tea (Camellia sinensis)Eepicatechins (ECG, EGC and EGCG). E-Cam 2009; 6:523-30. https://doi.org/10.1093/ecam/nem140
26. Huang HP, Shih YW, Chang YC, Hung CN, Wang CJ. ChemoOinhibitory effectTof mulberryYanthocyaninson melanomaAmetastasis involved in the Ras/PI3K pathway. J Agri Food Chem 2008; 56(19):9286-93. https://doi.org/10.1021/jf8013102
27. Chen PN, Hsieh YS, Chiou HL, Chu SC. SilibininNinhibits cell invasionNthrough inactivationNof both PI3K-Akt and MAPK signalingGpathways. Chemico Biological Interactions 2005; 156(2-3):141-50. https://doi.org/10.1016/j.cbi.2005.08.005
28. Vieira JRC, De Souza IA, Do Nascimento SC, Leite SP. IndigoferaAsuffruticosa: an alternativeEanticancertherapy. E-Cam 2007; 4(3):355-9. https://doi.org/10.1093/ecam/nel102
29. Vijayalakshmi A, Masilamani K, Nagarajan E and Ravichandiran V. In vitro antioxidant and anticancer activity of flavonoids from Cassia Tora linn. leaves against human breast carcinoma cell lines. Der Pharma Chemica 2015; 7(9):122-29.
30. Sandhia . K. G, Bindu A. R. Phytochemical, Anti-inflammatory and in vitro anticancer activities of Caesalpiniabunduc stem bark. Int J Pharma Sci and Res 2015; 6(1):50-56.
31. Khan PK, Sinha SP. Antimutagenic profile of antioxidant vitamins in drosophila mutation test. Biomed Environ Sci 2008; 21: 163-6. https://doi.org/10.1016/S0895-3988(08)60023-9
32. Yahaya G, Faten AE, Fred W, Hany AES. Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pac J Trop Med 2014; 7(1):355-63. https://doi.org/10.1016/S1995-7645(14)60258-3
33. Hirano TK, Oka M, Akiba M. Antiproliferative effect of synthetic and naturally occurring flavonoids on tumour cells of human breast carcinoma cell lines, ZR-75-1. Res Commun Chem Pathol Pharmacol 1989; 64:69-78.
34. Farkhondeh N, Abbas AD, Bahman E, Vahid M and Sepideh M. Cytotoxic properties of some medicinal plant extracts from mazandaran, Iran. Iran Red Crescent Med J 2013; 15(11):8871. https://doi.org/10.5812/ircmj.8871
35. Barrales Cureño HJ, Reyes Reyes C , Cortez Ruiz JA , Luna Cruz A , Andrade Hoyos P4 , E. Zaragoza Ruiz E et al. Current applications of MTT assays conducted under microscopic cytotoxicity analysis in cancerous cells by using therapeutic agents of plant origin; Microscopy and imaging science: practical approaches to applied research and education. (Cited on 29.03.2018) Available on: http://www.microscopy7.org/book/81-88.pdf.
36. Senthilkumar R, Manivannan R, Balasubramaniam A, Sivakumar T, Rajkapoor B. Effects of ethanol extract of Pisonia aculeate Linn. on ehrlich ascites carcinoma tumour bearing mice. Int J Green Pharm 2008; 2(1):50-3. https://doi.org/10.4103/0973-8258.39166
37. Jostein D, Egil K, Trond S. Bystander effects in UV-induced genomic instability: Antioxidants inhibit delayed mutagenesis induced by ultraviolet A and B radiation. J Carcinogenesis 2005; 4(11):1-9.
38. Demetrios S, Spyridon O, Demetrios K. Plant phenolics protect from bleomycin-induced oxidative stress and mutagenicity in Salmonella typhimurium TA102. Anticancer Research 2004; 24(2B):743-6
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