The ethanol extract of Muntingia calabura exhibits in vitro antidiabetic potentials by inhibiting carbohydrate digestive enzymes

In vitro hypoglycemic activity of Muntingia calabura

Authors

Abstract

Objective: The present research study was conducted to investigate in vitro anti-diabetic potentials of ethanol extract of leaves of Muntingia calabura for α-glucosidase and α-amylase inhibitory activities.

Materials & Methods: The leaves of Muntingia calabura were collected and authenticated. dried and powdered. The powdered drug was defatted using petroleum ether and extracted with ethanol. The ethanol extract of Muntingia calabura was subjected to preliminary phytochemical investigation. The in vitro α-glucosidase inhibitory and α-amylase inhibitory properties were determined for the ethanol extract and concentrations of extract required to inhibit absorbance (IC50 values) EEMC were determined.

Results: Significant IC50 values were found in both tests that assessed the inhibitory activity of α-glucosidase and α-amylase, demonstrating the capacity of the ethanol extract of Muntingia calabura to block carbohydrate digesting enzymes and prevent postprandial blood glucose.

Conclusion: The results of the presents investigation recommends that ethanol extract of Muntingia calabura possess significant α-glucosidase and α-amylase inhibitory activity.

Keywords: Antidiabetic activity, Muntingia calabura, α-glucosidase and α-amylase and IC50values.

Keywords:

Antidiabetic activity, Muntingia calabura, α-glucosidase and α-amylase, IC50values

DOI

https://doi.org/10.22270/jddt.v13i11.6276

Author Biographies

Subhajit Dutta, Department of Pharmacology, East West College of Pharmacy, Bangalore-560091, India

Department of Pharmacology, East West College of Pharmacy, Bangalore-560091, India

C Ramesh, Department of Pharmacology, East West College of Pharmacy, Bangalore-560091, India

Department of Pharmacology, East West College of Pharmacy, Bangalore-560091, India

A Madhu, Department of Pharmacology, East West College of Pharmacy, Bangalore-560091, India

Department of Pharmacology, East West College of Pharmacy, Bangalore-560091, India

Krishna Pundi, Department of Pharmaceutical Chemistry, East West College of Pharmacy, Bangalore-560091, India

Department of Pharmaceutical Chemistry, East West College of Pharmacy, Bangalore-560091, India

B.A. Sowmya, Department of Pharmacology, East West College of Pharmacy, Bangalore-560091, India

Department of Pharmacology, East West College of Pharmacy, Bangalore-560091, India

References

Linda S. BRS physiology. 4th Ed. Constanzo; Lippincott publishers:2006:255-6.

Tortara GJ & B Derrickson. Principles of anatomy and physiology; The digestive system. 13 ed. USA;2011.:967-1023.

Pyntngenlang L, Ramesh C, Rawal P, Sowmya BA. In vitro α-Glucosidase and α-amylase inhibitory activity of ethanol extract of Rhusmysorensis. Journal of Pharmaceutical Sciences and Research. 2023 Jun 1;15(6):1156-60.

Ramesh C, Rani AP. In vivo and in vitro antidiabetic potentials of methanol extract of Tephrosia pumila against alloxan-induced diabetes in experimental animals. International Journal of Health Sciences. 2019 May;13(3):10.

Shwetha S, Ramesh C, Pinkey R, Sowmya BA, Maria LA, Ranjitha CJ. The ethanol extract of Euphorbia hirta exhibits in vitro antidiabetic potentials by sensitization of the insulin. Journal of Drug Delivery and Therapeutics. 2023 Sep 15;13(9):125-7. https://doi.org/10.22270/jddt.v13i9.6209

Buhian WPC, et al. Bioactive metabolite profiles and antimicrobial activity of ethanolic extracts from Muntingia calabura L. leaves and stems. Asian Pacific Journal of Tropical Biomedicine. 2016; 6(8): 682-685. https://doi.org/10.1016/j.apjtb.2016.06.006

Aligita W, et al. Antidiabetic activities of Muntingia calabura L. leaves water extract in type 2 diabetes mellitus animal models. The Indonesian Biomedical Journal. 2018;10(2): 165-170. https://doi.org/10.18585/inabj.v10i2.405

Ramesh C, Alam A, Murtuja G, Hedayetullah M. Assessment of in vitro antioxidant and cytotoxic potentials of ethanol extract of Nymphaeanauchali. Journal of Drug Delivery and Therapeutics. 2022 Mar 15;12(2):43-7. https://doi.org/10.22270/jddt.v12i2.5234

Sushmitha DH, Ramesh C, Sowmya BA, SushmaBai BR, Rawal P, Shabana SU. Attenuation of cisplatin induced myelosuppression by methanol extract of Cedrusdeodara in Wistar rats. Journal of Complementary and Integrative Medicine. 2022 Mar 24;19(4):975-85. https://doi.org/10.1515/jcim-2021-0515 PMid:35325515

Ramesh C, Rani AP. In vivo and in vitro evaluation of Tephrosia calophylla for anti-diabetic properties. Int J Pharm Pharm Sci. 2018;10:138-44. https://doi.org/10.22159/ijpps.2018v10i6.25551

Ramesh C, Rani AP. In vivo and in vitro evaluation of Tephrosia calophylla for anti-diabetic properties. Int J Pharm Pharm Sci. 2018;10:138-44. https://doi.org/10.22159/ijpps.2018v10i6.25551

Puls W, Keup U, Krause HP, Thomas G, Hoffmeister F. Glucosidase inhibition. A new approach to the treatment of diabetes, obesity, and hyperlipoproteinaemia. Naturwissenschaften.1977;64:536-7. https://doi.org/10.1007/BF00483562 PMid:927538

Ramesh C, Ghosh K, Rawal P, Pramanik S. In vivo antioxidant and hypoglycaemic potentials of Rivina humilis extract against streptozotocin induced diabetes and its complications in wistar rats. Journal of Diabetes & Metabolic Disorders. 2023 Aug 17:1-1.

Chiba S. Molecular mechanism in a-glucosidase and glucoamylase. BiosciBiotechnolBiochem1997;61:1233-9. https://doi.org/10.1271/bbb.61.1233 PMid:9301101

Muhammad AP, Pinkey R, Ramesh C, Chaitra G. Evaluation of Antidiarrheal properties of ethanol extract of Brassica juncea in experimental animals. Journal of Drug Delivery and Therapeutics. 2021 Apr 15;11(2-S):19-23. https://doi.org/10.22270/jddt.v11i2-S.4615

DeFronzo RA, Jacot E, Jequier E, Maeder E, Wahren J, Felber JP. The effect of insulin on the disposal of intravenous glucose. Results from indirect calorimetry and hepatic and femoral venous catheterization. Diabetes. 1981;30:1000-7. https://doi.org/10.2337/diab.30.12.1000 PMid:7030826

Ravichandra VD, Ramesh C, Sridhar KA. Hepatoprotective potentials of aqueous extract of Convolvulus pluricaulis against thioacetamide induced liver damage in rats. Biomedicine & Aging Pathology. 2013 Jul 1;3(3):131-5. https://doi.org/10.1016/j.biomag.2013.06.005

Cohen G, Heikkila KE. Generation of hydrogen peroxide, superoxide radical by 6-hydroxy dopamine, dialuric acid and related agents. J Biol and Chem 1974; 49:2447-52. https://doi.org/10.1016/S0021-9258(19)42751-8

PinkeyRawal, RC, Sowmya BA, Ranjitha CJ. Determination of lipid lowering properties of methanol extract of Tephrosia villosa against high fat diet induced hyperlipidemia in wistar rats.

Amir S, JhaAitya GK, Ramesh C, Chaturvedi SA, MdSahidulla and Kiran P. Ethanol extract of Embelia ribes stimulates in vitro glucose uptake by peripheral tissues. Euro J Biomed & Pharma Sci 2022;9(11):177-81.

Takasu N, Asawa T, Komiya I, Nagaswa Y, Yamada T. Alloxan-induced DNA strand breaks in pancreatic islets:Evidence for H2O2 as an intermediate. J Biol and Chem 1991;266:2112-4. https://doi.org/10.1016/S0021-9258(18)52216-X

Sowmya BA, Ramesh C, Rawal P, Ranjitha CJ. Amelioration of high fat diet induced hyperlipidemia by methanol extract of Tephrosia calophylla in wistar rats. Journal of Pharmaceutical Sciences and Research. 2023 Jul 1;15(7):1191-3.

Published

15-11-2023
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How to Cite

1.
Dutta S, Ramesh C, Madhu A, Pundi K, Sowmya B. The ethanol extract of Muntingia calabura exhibits in vitro antidiabetic potentials by inhibiting carbohydrate digestive enzymes: In vitro hypoglycemic activity of Muntingia calabura. J. Drug Delivery Ther. [Internet]. 2023 Nov. 15 [cited 2025 Feb. 14];13(11):26-30. Available from: https://jddtonline.info/index.php/jddt/article/view/6276

How to Cite

1.
Dutta S, Ramesh C, Madhu A, Pundi K, Sowmya B. The ethanol extract of Muntingia calabura exhibits in vitro antidiabetic potentials by inhibiting carbohydrate digestive enzymes: In vitro hypoglycemic activity of Muntingia calabura. J. Drug Delivery Ther. [Internet]. 2023 Nov. 15 [cited 2025 Feb. 14];13(11):26-30. Available from: https://jddtonline.info/index.php/jddt/article/view/6276