Evaluation of Antidiarrheal properties of ethanol extract of Brassica juncea in experimental animals

  • Aslam P Muhammad Department of Pharmacology, East West College of Pharmacy, Bangalore-560091
  • Rawal Pinkey Department of Pharmaceutical chemistry, East West College of Pharmacy, Bangalore-560091
  • C Ramesh Department of Pharmacology, East West College of Pharmacy, Bangalore-560091
  • G Chaitra Department of Pharmaceutics, East West College of Pharmacy, Bangalore-560091

Abstract

Objective: The present study was designed to evaluate the anti-diarrheal potentials of ethanol extract of Brassica juncea in albino rats. 


Materials and methods: The defatted powder of whole plant of Brassica juncea subjected to extraction using ethanol in soxhlet apparatus.  The acute oral toxicity was studied for ethanol extract according to OECD guidelines 425. The antidiarrheal properties of ethanol extracts was assessed against castor oil induced diarrhea, magnesium sulfate induced diarrhea and charcoal passage test in albino wistar rats.


Results: The ethanol extract of Brassica juncea has effectively reversed diarrhea. The extract at higher dose 400mg/kg shows significant reduction the number of feces against castor oil induced and MgSO4 induced diarrhea. In charcoal passage test, higher dose of ethanol extract also shown significant reduction in the distance travelled.


Conclusion: The result obtained establish the efficiency of ethanol extract of Brassica juncea as antidiarrheal agent


Keywords: Brassica juncea, castor oil, magnesium sulphate, charcoal passage test.

Keywords: Brassica juncea, castor oil, magnesium sulphate, charcoal passage test

Downloads

Download data is not yet available.

Author Biographies

Aslam P Muhammad, Department of Pharmacology, East West College of Pharmacy, Bangalore-560091

Department of Pharmacology, East West College of Pharmacy, Bangalore-560091

Rawal Pinkey, Department of Pharmaceutical chemistry, East West College of Pharmacy, Bangalore-560091

Department of Pharmaceutical chemistry, East West College of Pharmacy, Bangalore-560091

C Ramesh, Department of Pharmacology, East West College of Pharmacy, Bangalore-560091

Department of Pharmacology, East West College of Pharmacy, Bangalore-560091

G Chaitra, Department of Pharmaceutics, East West College of Pharmacy, Bangalore-560091

Department of Pharmaceutics, East West College of Pharmacy, Bangalore-560091

References

1. Reena G, Jitendra G. Investigation of Antidiarrhoeal Activity of Ethanolic Extract of Tamarindus indica L. Seeds in Albino Wistar Rats, Asian J of Pharmaceutics. 2016; 10:492.
2. Poonam G, Daswani, S. Brijesh, Pundarikakshudu T, Noshir HA, Tannaz J. Antidiarrhoeal activity of Zingiber officinale (Rosc.). Current science. 2010; 98(2):224-2.
3. Belay M, Assefa BA, Zewdu BW. Antidiarrheal Activity of 80% Methanolic Leaf Extract of Justicia schimperiana, Hindawi. 2018; 2018:12.
4. Parikh H. Pharmacognosy and Phytochemical Analysis of Brassica juncea Seeds, Pharmacognosy Journal. 2014; 5(6):49-51.
5. Thakur, et all. Antidepressant-like effects of Brassica juncea L, leaves in diabetic rodents. Indian J of Exp Biology. 2014; 52:613-22.
6. Kokate CK. Practical Pharmacognosy. Vol. 4. New Delhi: Vallabh Prakashan; 1994. p110-1.
7. Khandelwal KR. Practical Pharmacognosy-Techniques and Experiments. Pune: Nirali Prakashan; 2000.
8. OECD. Acute Oral Toxicity-Acute Oral Toxic Class Method. Guideline No. 423, Adopted 23.03.1996. In: Eleventh Addendum to the OECD Guidelines for the Testing of Chemicals. Organisation for Economic Co-operation and Development, Paris: OECD; 2000.
9. Karanayil R, Barij N, Aiyolu R. Antidiarrheal activity of Capparis zeylanica leaf extracts; J Adv Pharm Technol Res. 2011; 2(1):39–42.
10. Edlam T at all. Evaluation of the anti-diarrheal activity of the aqueous stem extract of Lantana camara Linn (Verbenaceae) in mice. BMC Complement Altern Med. 2017; 17: 190.
11. Amsalu D, Ephrem E, Workineh S. Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model, BMC Com Alt Medicine. 2016; 16:379.
12. Gurram SK, Lakshmi SK, Govindarao M, Karthik YP, Sudha BC, Wound Healing Activity Of Ethanolic Seed Extract Of Brassica Juncea, Int J Pharmacy 2013; 3(2):364-368.
13. Rode MS, Kalaskar MG, Gond NY, Surana SJ. Evaluation of anti-diarrheal activity of Diospyros malabarica bark extract, Bangladesh J Pharmacol 2013; 8:49-53.
14. Deg U, Engidawork E, Shibeshi W. Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model, Comp and Alt Med 2016; 16:379.
15. NoYuji S, Ju-Xiu HE, Hidemasa N, Tadato T, Teruaki A. Isoliquiritigenin, one of the antispasmodic principles of Glycyrrhiza ularensis roots, acts in the lower part of intestine. Biol Pharm Bull. 2007; 30:145-9.
16. DeHaven-Hudkins DL, Burgose LC, Cassel JA, Daubert JD, DeHaven RN, Mansson E. Loperamide (ADL 2-1294), an opioid antihyperalgesic agent with peripheral selectivity. J Pharmacol and Expl Therap 1999; 289(1):494-502.
17. OB Balemba Y, Bhattarai C, Stenkamp S, GM.Mawe, Thetraditional antidiarrheal remedy Garcinia buchananii stem bark extract inhibits propulsive motility and fast synaptic potentials in the guinea pig distal colon Neuro-gastroenterol & Mot 2010; 22(12):1332–9.
Crossmark
Statistics
384 Views | 273 Downloads
How to Cite
1.
Muhammad AP, Pinkey R, Ramesh C, Chaitra G. Evaluation of Antidiarrheal properties of ethanol extract of Brassica juncea in experimental animals. JDDT [Internet]. 15Apr.2021 [cited 24Jun.2024];11(2-S):19-3. Available from: https://jddtonline.info/index.php/jddt/article/view/4615