Qualitative and quantitative determination of secondary metabolites and antioxidant potential of Nymphaea nouchali flowers
Abstract
In the Indian ayurvedic system of medicine, Nymphaea nouchali is used for the treatment of diabetes, inflammation, liver disorders, cutaneous diseases, blenorrhagia, urinary disorders, menorrhagia, menstruation problem, as an aphrodisiac, bitter tonic, antimicrobial agent and anti antihepatotoxic effect. The aim of the present study is to examine Nymphaea nouchali flowers for phytochemical profile, in vitro antioxidant activities. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The In vitro antioxidant activity of ethanolic extract of the flowers was assessed against nitric oxide, hydrogen peroxide assay using standard protocols. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of flowers ethanolic extract was (18.4 mg/100mg), followed by flavonoids (12.4mg/100mg). The activities of ethanolic flowers extract against nitric oxide and hydrogen peroxide were concentration dependent with IC50 values of 68.39 and 64.54 μg/ml respectively. The present study concluded that the crude extract of Nymphaea nouchali is a potential source of natural antioxidants and this justifies its use in folkloric medicine.
Keywords: Nymphaea nouchali, Phytochemical, Antioxidant, Nitric oxide, Hydrogen peroxide, Phenols, Flavonoids
DOI
https://doi.org/10.22270/jddt.v8i6-s.2095References
Sreeramulu D, Reddy CVK, Chauhan A, Balakrishna N, Raghunath M, Natural antioxidant activity of commonly consumed plant foods in India: Effect of domestic processing, Oxidative Medicine and Cellular Longevity, 2013; doi: 10.1155/2013/369479.
Hamid AA, Aiyelaagbe OO, Usman LA, Ameen OM, Lawal A, Antioxidants: Its medicinal and pharmacological applications, African Journal of Pure and Applied Chemistry, 2010; 4(8):142-151.
Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J, The role of oxidative stress and antioxidants in diabetic complications, Sultan Qaboos University Medical Journal 2012; 12(1):5-18.
Padmaja M, Sravanthi M, Hemalatha KPJ, Evaluation of antioxidant activity of two Indian medicinal plants, Journal of Phytological Research, 2011; 3(3):86-91.
Branen AL, Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene, Journal of the American Oil Chemists' Society, 1975; 52(2):59-63.
Kratchanova M, Denev P, Ciz M, Lojek A, Mihailov A, Evaluation of antioxidant activity of medicinal plants containing polyphenol compounds. Comparison of two extraction systems, Acta Biochimica Polonica, 2010; 57(2):229-34.
Bhandarkar MR, Khan A, Antihepatotoxic effect of Nymphaea stellata Willd, against carbon tetrachloride-induced hepatic damage in albino rats, Journal of Ethnopharmacology, 2004; 91(1):61-64.
Raja MKMM, Agilandeswari D, Madhu BH, Mallikarjuna M, Sowjanya PJS, Aphrodisiac activity of ethanolic extract of Nymphaea stellata leaves in male rats, Contemporary investigations and observations in pharmacy, 2012; 1(1):24-30.
Ishrat J, Mamun MAA, Hossen MA, Sakir JAMS, Shamimuzzaman M, Uddin MJ, et al., Antioxidant, analgesic and anti-inflammatory activities of Nymphaea nouchali flowers, Research Journal of Pharmacology, 2012; 6(5):62-70.
Nagavani V, Rao TR, Evaluation of antioxidant potential and qualitative analysis of major polyphenols by RP-HPLC in Nymphaea nouchali brum flowers, International Journal of Pharmacy and Pharmaceutical Sciences, 2010; 2(4):98-104.
Huang YN, Zhao YL, Gao XL, Zhao ZF, Jing Z, Zeng WC, et al., Intestinal alpha-glucosidase inhibitory activity and toxicological evaluation of Nymphaea stellata flowers extract, Journal of Ethnopharmacology, 2010; 131(2):306-312.
Rajagopal K, Sasikala K, Antihyperglycaemic and antihyperlipidaemic effects of Nymphaea stellata in alloxan induced diabetic rats, Singapore Medicinal Journal, 2008; 49(2):137-141.
Kiran Kumar A, Sai B, Ammani K, Antimicrobial and phytochemical analysis of Nymphaea nauchali leaf extracts, International Journal of Research and Reviews in Applied Sciences, 2(2):142-151.
Chowdhury BN, Haque MM, Sohrab MH, Afroz F, Al-mansur MA, Sultana T, et al., Steroids from the stem of Nymphaea stellata, Journal of the Bangladesh Academy of Sciences, 2013; 37(1):109-113.
Raja MKMM, Sethiya NK, Mishra SH, A comprehensive reiview on Nymphaea stellata: A traditionally used bitter, Journal of Advanced Pharmaceutical Technology & Research, 2010; 1(3):311-319.
Kizu H, Tomimori T, Phenolic constituents from the flowers of Nymphaea stellata, Nature Medicine, 2003; 57(3):118.
Kapoor VP, Khan PSH, Raina RM, Farooqi MIH, Chemical analysis of seeds from 40 non- leguminous species, Part III, Science as Culture, 1975; 41:336-339.
Mukherjee PK. Quality control of herbal drugs. 2nd Ed. Business Horizons; 2007.
Khandelwal KR. Practical pharmacognosy technique and experiments. 23rd Ed. Nirali Prakashan; 2005.
Kokate CK. Practical pharmacognosy. 4th Ed. Vallabh Prakashan; 1994.
Olufunmiso OO, Afolayan AJ, Phenolic content and antioxidant property of the bark extract of Ziziphus mucronata willd. Subsp. mucronata willd, BMC Complement Alternative Medicine, 2011; 11:130.
Marcocci L, Maguire JJ, Droy MT, The nitric oxide scavenging properties of Gingo biloba extract EGb 761, Biochemical and Biophysical Research Communications, 1994; 15:748-755.
Czochra MP, Widensk AJ, Spectrophotometric determination of H2O2 activity, Analytica Chimica Acta, 2002; 452:177-84.
Pandey KB, Rizv SI, Plant polyphenols as dietary antioxidants in human health and disease, Oxidative Medicine and Cellular Longevity. 2009; 2(5):270-278.
Li H, Wang Z, Liu Y, Review in the studies on tannins activity of cancer prevention and anticancer, Zhong-Yao-Cai Chinese. 2003; 26(6):444-448.
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