Synthesis, Characterisation, Invitro anticancer activity of Diazo Derivatives of 1,3,4-oxadiazole
Abstract
This study delves into the comprehensive evaluation of newly synthesized Diazo Derivatives of 1,3,4-oxadiazole compounds, including Aniline, Ortho nitro aniline, Meta nitro aniline, Ortho anisidine, Meta anisidine, 3-chloro aniline, 2,6-dimethyl aniline, 2,5- dicloro aniline, and 3,5- dichloro aniline, aiming to unravel their potential as antioxidants and anticancer agents. The assessment of antioxidant potential involved the utilization of the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The cytotoxic activity of these compounds was assessed against MCF-7 breast cancer cells using the MTT assay. Morphological assessments using acridine orange/ethidium bromide (AO/EB) staining. Results were expressed as IC50 values, with 2,5-dichloro aniline and 3-chloro aniline demonstrating remarkable antioxidant activity, each with an IC50 value of 62.5 µg/mL. Several other compounds also exhibited significant antioxidant properties, albeit with slightly higher IC50 values. Notably, Ortho anisidine and Ortho nitro aniline displayed no substantial inhibitory activity against oxidative stress. The cytotoxic activity of these compounds was assessed against MCF-7 breast cancer cells using the MTT assay, with IC50 values ranging from 62.5 to 1000 µg/mL. 2,5-dichloro aniline and 3-chloro aniline emerged as potent inhibitors, both achieving an IC50 value of 62.5 µg/mL. Aniline, Meta nitro aniline, Meta anisidine, 2,6-dimethyl aniline, and 3,5-dichloro aniline exhibited intermediate inhibitory activity, while Ortho anisidine and Ortho nitro aniline showed no significant inhibition. Morphological assessments using acridine orange/ethidium bromide (AO/EB) staining revealed diverse cellular responses induced by 2,5-dichloro aniline and 3-chloro aniline treatment. These changes align with well-established apoptotic processes observed in therapeutically treated cell lines. This research contributes valuable insights into the antioxidant and anticancer potential of Diazo Derivatives of 1,3,4-oxadiazole compounds, with 2,5-dichloro aniline and 3-chloro aniline emerging as promising candidates for further exploration in combatting oxidative stress and breast cancer. These findings underscore the importance of antioxidants in cancer prevention and the potential therapeutic applications of these compounds.
Keywords: Diazo Derivatives of 1,3,4-oxadiazole, 2,5-dichloro aniline, 3-chloro aniline, DPPH, MTT assay, MCF-7
Keywords:
Diazo Derivatives of 1,3,4-oxadiazole, 2,5-dichloro aniline, 3-chloro aniline, DPPH, MTT assay, MCF-7DOI
https://doi.org/10.22270/jddt.v13i10.5984References
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