Assessment of antibacterial activity of some extracts of Securinega virosa (Roxb. ex Willd.) Baill on pathogens bacteria

  • Mani Adrien KOUANGBE Department of Microbiology and Molecular Biology, Training and Research Unit of Agriculture, fisheries resources and agro-industry, University of San Pedro, San Pedro, Côte d’Ivoire
  • Messou TCHUMOU Department of Microbiology and Molecular Biology, Training and Research Unit of Agriculture, fisheries resources and agro-industry, University of San Pedro, San Pedro, Côte d’Ivoire
  • Monon KONE Department of Biochemistry-Genetics, Training and Research Unit of Biological Sciences, Peleforo Gon Coulibaly University of Korhogo, Korhogo, Côte d'Ivoire.
  • Karamoko OUATTARA Department of Microbiology and Molecular Biology, Training and Research Unit of Agriculture, fisheries resources and agro-industry, University of San Pedro, San Pedro, Côte d’Ivoire
  • Jean David N’GUESSAN Department of Biology and Health, Training and Research Unit of Bioscience, University of Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire

Abstract

Today, faced with the emergence of microbial resistance to antibiotics, the renewal of the arsenal of anti-infective drugs is acutely posed. In order to explore other sources of anti-infective drugs, this study therefore proposed to evaluate the antibacterial activity in vitro of several extracts of Securinega virosa, a well-known combretacea of populations in northern Côte d'Ivoire. To achieve this objective, the method of determining the diameters of the zones of inhibition on wells in an agar medium as well as that of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration were carried out. The results showed that the aqueous extract was not active on all the bacteria tested. The dichloromethane fraction at 500 mg/mL inhibited growth of Shigella Typhi (12.5 mm), followed by Streptococcus sp. (12.33 0.25 mm) and Staphylococcus aureus Meti-R (11.75 1.25 mm). The ethyl acetate fraction inhibited primarily Gram-positive bacteria with average diameters of 12 mm to 500 mg/mL. The ethanol fraction was most active on all bacteria with inhibition diameters ranging from 9 to 13.33 mm to 500 mg/mL. It showed the lowest MIC (3.12 mg/mL) on gram-positive and large Gram-negative levels ranging from 12.5 to 50 mg/mL. This study through its results provides data in favor of the traditional use of Securinega virosa in therapy.


Keywords : Securinega virosa, in vitro, antibacterial, activity

Keywords: Securinega virosa, in vitro, antibacterial, activity

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Author Biographies

Mani Adrien KOUANGBE, Department of Microbiology and Molecular Biology, Training and Research Unit of Agriculture, fisheries resources and agro-industry, University of San Pedro, San Pedro, Côte d’Ivoire

Department of Microbiology and Molecular Biology, Training and Research Unit of Agriculture, fisheries resources and agro-industry, University of San Pedro, San Pedro, Côte d’Ivoire

Messou TCHUMOU, Department of Microbiology and Molecular Biology, Training and Research Unit of Agriculture, fisheries resources and agro-industry, University of San Pedro, San Pedro, Côte d’Ivoire

Department of Microbiology and Molecular Biology, Training and Research Unit of Agriculture, fisheries resources and agro-industry, University of San Pedro, San Pedro, Côte d’Ivoire

Monon KONE, Department of Biochemistry-Genetics, Training and Research Unit of Biological Sciences, Peleforo Gon Coulibaly University of Korhogo, Korhogo, Côte d'Ivoire.

Department of Biochemistry-Genetics, Training and Research Unit of Biological Sciences, Peleforo Gon Coulibaly University of Korhogo, Korhogo, Côte d'Ivoire.

Karamoko OUATTARA, Department of Microbiology and Molecular Biology, Training and Research Unit of Agriculture, fisheries resources and agro-industry, University of San Pedro, San Pedro, Côte d’Ivoire

Department of Microbiology and Molecular Biology, Training and Research Unit of Agriculture, fisheries resources and agro-industry, University of San Pedro, San Pedro, Côte d’Ivoire

Jean David N’GUESSAN, Department of Biology and Health, Training and Research Unit of Bioscience, University of Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire

Department of Biology and Health, Training and Research Unit of Bioscience, University of Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire

References

1- Global Health Metrics. Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990 - 2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet, 2018; 392:1789-1858. https://doi.org/10.1016/S0140-6736(18)32279-7
2- Murray-DPhil L, Ikuta KSMD, Sharara FMS, Swetschinski LMSc, Gray ABS, Han BAC, Bisignano CMPH, Rao MPHP, Wool MPHE, Johnson MScSC, Albertson BSS, Aravkin A et al. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet, 2022; 399(10325) : 629-655. https://doi.org/10.1016/S0140-6736(21)02724-0
3- O'Neill J. Tackling drug-resistant infections globally: final report and recommendations. London, Review on Antimicrobial Resistance, 2016 ; p 84 .
4- Lewis K, Ausubel FM. Prospects for plant derived antibacterials. Nat Biotechnol, 2006; 24(12):1504-7 https://doi.org/10.1038/nbt1206-1504
5- Cowan MM. Plant Products as Antimicrobial Agents. Clin Microbiol Rev. 1999; 12(4):564-582 https://doi.org/10.1128/CMR.12.4.564
6- Bereksi MS, Hassaïne H, Bekhechi C, Abdelouahid DE. Evaluation of Antibacterial Activity of Some Medicinal Plants Extracts Commonly Used in Algerian Traditional Medicine against Some Pathogenic Bacteria. Pharmacog J. 2018; 10(3):507-12. https://doi.org/10.5530/pj.2018.3.83
7- Koné M, Ouattara K, Gnahoue G, Ouattara A. Coulibaly A. Study of ethnopharmacological and phytochemical screening of some plants involved in the treatment of abdominal infections in the department of Kouto (Côte d'Ivoire). Sch J App Med Sci, 2013; 1(2):56-61. https://doi.org/10.36347/sjams.2013.v01i02.007
8- Tanko Y, Okasha MA, Magaji GM. Anti-diabetic properties of Securinega virosa (Euphorbiaceae) leaf extract. Afr J Biotechnol, 2008; 7(1):22-24.
9- Magaji MG, Yaro AH, Musa AM, Anuka JA, Abdu-Aguye I. Hussaini IM. Central depressant activity of butanol fraction of Securinega virosa root bark in mice. J Ethnopharmacol, 2012; 141(1):128-133. https://doi.org/10.1016/j.jep.2012.02.010
10- Chauke AM, Shai LJ, Mphahlele PM, Mogale MA. Radical scavenging activity of selected medicinal plants from Limpopo Province of South Africa. Afr J Tradit Complement Altern Med, 2012; 9(3):426-430. https://doi.org/10.4314/ajtcam.v9i3.19
11- Sanon S, Gansana A, Ouattara LP, Traore A, Issa N, Tiono OA, Taramelli D, Basilico N, Sirima SB. In vitro antiplasmodial and cytotoxic properties of some medicinal plants from western Burkina Faso. Afr J Lab Med, 2013; 2(1):1-7. https://doi.org/10.4102/ajlm.v2i1.81
12- Guede-Guina F. Potencies of MISCA, a plant source concentrate against fungi. Mycol Med. 1993; 5:225-229.
13- Manga A, Gassama A, Sy YG, Bassene E, Lavaud C. Structural determination of news flavones C-glycosides and trans (S, E) - (-) clovamide isolated Icacina senegalensis Juss leaves (Icacinaceae). J. Soc. Ouest-Afr. Chim ; 2013; 035:15-27.
14- Daoud A, Malika D, Bakari S, Hfaiedh N, Mnafgui K, Kadri A, et al. Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of date palm pollen (DPP) from two tunisian cultivars. Arab. J. Chem. 2015; in press https://doi.org/10.1016/j.arabjc.2015.07.014
15- Black JG, Black LJ. Microbiology: Principles and Explorations; John Wiley & Sons: Hoboken, NJ, USA, 2018; p896
16- Khaleel AI, Sijam K, Rashid TS, Ahmad KB. Phytochemical determination and antibacterial activity of Punica granatum peel extracts against plant pathogenic bacteria. Am J Plant Sci, 2016; 7:159-166. https://doi.org/10.4236/ajps.2016.71017
17- Onzo CF, Azokpota P, Dah-Nouvlessounon D, Toure H, Adjatin A, Baba-Moussa L. Évaluation de l'activité antimicrobienne de quatre feuilles utilisées comme emballages dans l'artisanat agroalimentaire au Bénin. J. Appl. Biosci. 2015; 95:9015-9027 https://doi.org/10.4314/jab.v95i1.11
18- Patel JP, Gami B, Patel K. Solanki R. Antibacterial activity of methanolic and acetone extract of some medicinal plants used in indian folklore. Int. J. Phytomedicine, 2011; 3:261-269.
19- Sukesh K, Shafi TT, Densingh J. Phytochemical investigation and antibacterial activity of Gymnema sylvestre and Andrographis paniculata from western ghats. Int. J. Phytomedicine, 2011; 3:254-260
20- Yala JF, Ntsameso-Mve-Mba V, Azzizet IY, Lepengue NAl, Souza A. Évaluation in vitro de l'activité antimicrobienne de l'extrait aqueux de Eryngium foetidium récolté dans la ville de Franceville. J. Appl. Biosci, 2016; 103:9886-9893. https://dx.doi.org/10.4314/jab.v103i1.10
21- Bhalodia NR, Shukla VJ. Antibacterial and antifungal activities from leaf extracts of Cassia fistula : an ethnomedicinal plant. J. Adv. Pharm. Technol. Res. 2011; 2 :104-109. https://doi.org/10.4103/2231-4040.82956
22- Adejare OY, Oduyebo OO, Oladele RO, Nwaokorie FO, Ogunsola FT. In vitro antifungal effect of Garcinia lola and Garlic (Alliums sativu) on vaginal isolates of Candida. Afr. J. Cln. Exper. Microbiol, 2013; 14(3):140-145. https://doi.org/10.4314/ajcem.v14i3.4
23- Kouangbé MA, Bahi C, Tia, Boga GL, Edoh V, Djaman AJ, N'Guessan J. Antifungal activity of roots barks extract of Securinega virosa (Roxb. ex Willd.) Baill and Anogeissus leiocarpa (DC.) Guill. & Perr, two plants used in the traditional treatment of candidiasis in northern Côte d'Ivoire. JBcRR, 2015; 8(1):1-11. https://doi.org/10.9734/IJBCRR/2015/17481
24- Credo D, Mabiki FP, Machumi F, Chacha M, Cornett C. Activity of sterols isolated from the leaves of Commiphora swynnertonii (Burtt) against some nosocomial infectious bacteria. J Phytopharmacol, 2022; 11(5):335-340. https://doi.org/10.31254/phyto.2022.11504
25- Dickson RA, Houghton PJ, Hylands PJ. S. (2006) Antimicrobial, resistance-modifying effects, antioxidant and free radical scavenging activities of Mezoneuron benthamianum Baill., Securinega virosa Roxb. &Wlld. and Microglossa pyrifolia Lam. Phytother Res. 2006; 20(1):41-45. https://doi.org/10.1002/ptr.1799
26- Amenu JD, Neglo D, Abaye DA. Comparative study of the antioxidant and antimicrobial activities of compounds isolated from solvent extracts of the roots of Securinega virosa. JBM, 2019; 7:27-41. https://doi.org/10.4236/jbm.2019.78003
27- Yéo SO, Guessennd KN, Ouattara K, Konan KF, Djaman AJ, Dosso M, Coulibaly A. Triphytochemistry and in vitro antibacterial activity of root extracts of Cochlospermum planchonii Hook f. ex. Planch (Cochlospermaceae) on multireristant strains. Sch Acad J Biosci, 2014; 2(10):663-670
28- Pandithurai M, Murugesan S, Sivamurugan V. Antibacterial activity of various solvent extracts of marine brown alga Spatoglossum asperum. Int. J. Pharmacol. Res., 2015; 5(6):133-138.
29- Orhue PO, Momoh ARM. Antibacterial activities of different solvent extracts of Carica papaya fruit parts on some gram positive and gram negative organisms. IJHPR, 2013; 2(4):42-47.
30- Enwa FO, Ikpefen EO, Damera C. The Effects of Securinega virosa leaves on Methicillin-Resistant Staphylococcus Aureus (MRSA), IJPRAS, 2021; 10(2):29-34 https://doi.org/10.51847/HAQZ2xRZFh
31- Elisha IL, Botha FS, McGaw LJ. et al. The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complement Altern Med, 2017; 17(133). https://doi.org/10.1186/s12906-017-1645-z
32- Tadeg H, Mohammed E, Asres K, Gebre-mariam T. Antimicrobial activities of some selected traditional Ethiopian medicinal plants used in the treatment of skin disorders. J. Ethnopharmacol, 2005; 100:168-175. https://doi.org/10.1016/j.jep.2005.02.031
33- Shegute T, Wasihun Y. Antibacterial Activity and Phytochemical Components of Leaf Extracts of Agave americana. J Exp Pharmacol, 2020; 12:447-454 https://doi.org/10.2147/JEP.S258605
34- Anarado CE, Anarado CJO, Umedum NL, Chukwubueze FM, Anarado IL. Phytochemical and antimicrobial analysis of leaves of Bridelia micrantha, Cassytha filiformis, Euphorbia hirta and Securinega virosa. J Pharmacogn Phytochem, 2020; 9(3):581-587.
35- Ezeabara CA, Gabriel SO. Phytochemical and in vitro antimicrobial assessments of various parts of Securinega virosa (Roxb. ex Willd.) Baill. Int. J. Biol. Sci, 2021; 8(5):35-38.
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KOUANGBE MA, TCHUMOU M, KONE M, OUATTARA K, N’GUESSAN JD. Assessment of antibacterial activity of some extracts of Securinega virosa (Roxb. ex Willd.) Baill on pathogens bacteria. JDDT [Internet]. 15Jan.2023 [cited 27Jan.2023];13(1):116-22. Available from: https://jddtonline.info/index.php/jddt/article/view/5726