Evaluation of the antiviral activity of different extracts of Gliricidia sepium and Xylopia aethiopica on enteroviruses isolated in Côte d'Ivoire
Abstract
Objective : The emergence of viral infections has highlighted the need for new antiviral molecules. This study is part of an exploration into finding new antiviral compounds from medicinal plants used in traditional pharmacopoeia in Côte d'Ivoire. The aim is to determine which of the extracts from Gliricidia sepium leaves and Xylopia aethiopica fruits (hydroalcoholic, hexane/water, ethyl acetate/water, aqueous) exhibits the best antiviral activity.
Methodology: The cytotoxicity of each partition P1 (hydroalcoholic), P2 (hexane/water), P3 (ethyl acetate/water), P4 (aqueous) of Gliricidia sepium leaves and P1' (hydroalcoholic), P2' (hexane/water), P3' (ethyl acetate/water), P4' (aqueous) of Xylopia aethiopica fruits is determined on RD cells. The antiviral activity of these partitions was then evaluated against a type 1 enterovirus.
Results: The hexane-water partition showed high antiviral activity against type 1 enterovirus, with an inhibition of 65.78±9.21% at a concentration of 1 μg/mL for Gliricidia sepium leaves, compared to a non-significant inhibition of 86.84±5.26% at 31.25 μg/mL for Xylopia aethiopica fruits. Additionally, the aqueous partition of Gliricidia sepium induced an inhibition of 50±0.01% at a concentration of 1.95 μg/mL.
Conclusion: Hexane-water and aqueous partitions of Gliricidia sepium showed́ potent antiviral activitý against enterovirus 1 than those of Xylopia. aethiopica.
Keywords: plant partition, RD cells, enterovirus type 1.
Keywords:
plant partition, RD cells, enterovirus type 1DOI
https://doi.org/10.22270/jddt.v14i10.6819References
Irwin K. K., Renzette N., Kowalik T. F. & Jensen J. D., 2016.Antiviral drug resistance as an adaptive process. Virus Evolution, 2016 ;2(1):vew014 https://doi.org/10.1093/ve/vew014
Amegawi K. K., De Souza C., Gbeassor M. & Koumaglo K.Etude de l'activite antimicrobienne des extraits aqueux totaux de dix plantes medicinales. Revue de Medecines et Pharmacopees Africaines,1993;109 115.
Koumaglo H. K., MacKinnon S., Awang D. V. C., Gbeassor M. A., Arnason J. T. & Durst T. Development of ethical phytomedicines for Togo, West Africa. the Institute of Development Studies and partner organisations,1996.
Anani K., Hudson J. B., De Souza C., Akpagana K., Tower G. H., Arnason J. T. & Gbeassor M.Investigation of medicinal plants of togo for antiviral and antimicrobial activities. Pharmaceutical Biology,2000;38(1):40 45 https://doi.org/10.1076/1388-0209(200001)3811-BFT040
Adjanohoun E. J. State of the evolution of African ethnopharmacopoeia; Bull. Med. Trad. Pharm.,1990;4(1):59-63.
Ake-Assi L. Traditional medicine and pharmacopoeia. Report on the international conference on traditional African medicine in Abidjan, Ivory Coast. Bull. Med. Trad. Pharm., ACCT,1991:4(2):203.
Bolou G. E.-K., Tra B. B. F., Yao K., Bouagnon J. J.-R., Lidji C. J. S. R., N'guessan C. D. R., N'guessan J. D., Zirihi G. N., Djaman A. J., Bolou G. E.-K., Tra B. B. F., Yao K., Bouagnon J. J.-R., Lidji C. J. S. R., N'guessan C. D. R., N'guessan J. D., Zirihi G. N. & Djaman A. J. Inventory of plants used in the treatment of viral diseases, sold on markets in the district of Abidjan. GSC Biological and Pharmaceutical Sciences,2022:19(1):078 090 https://doi.org/10.30574/gscbps.2022.19.1.0132
Bouagnon J. J. R., Bolou G. E. K., Guédé K. B., Sanga D., Koffi L. R., N'Guessan C. D. R., Konan Y., Adjogoua E. V., N'Guessan J. D., Djaman A. J. & Dosso M. Phytochemical study and evaluation of the antiviral activity of aqueous extracts of three medicinal plants; Xylopia aethiopica, Gliricidia sepium and Ocimum gratissimum used in Cote d'Ivoire. African Journal of Clinical and Experimental Microbiology, 2022;23(2):141 148 https://doi.org/10.4314/ajcem.v23i2.4
Zirihi G. N., Kra A. K. M. Evaluation of the antifungal activity of Microglossa pyrifolia (LARMARCK) O. KUNTZE (Asteraceae) "PYMI" on the in vitro growth of Candida albicans. Pharmacopoeia and traditional African medicine, 2003; 17:11-19.
Ouattara S., Kporou K. E., Kra A. K. M., Zirihi G. N., N'Guessan J. D., Coulibaly A. & Djaman A. J.,. Antifungal activities of Terminalia ivorensis A. Chev. bark extracts against Candida albicans and Aspergillus fumigatus. Journal of Intercultural Ethnopharmacology,2013;2:49-52. https://doi.org/10.5455/jice.20121205083931
Kra A.K.M., Ahon G.M., Djo-Bi D., Ouattara S., Coulibaly A. & Djaman A.J. . Antifungal activities of medicinal plants extracts of Ivorian pharmacopoeia. Journal of Intercult Ethnopharmacology,2014;3:159-166. https://doi.org/10.5455/jice.20140627125512
Sunday A., Orjiekwe C., Ehiagbonare J. & Arimah B. D.Preliminary phytochemical analysis and insecticidal activity of ethanolic extracts of four tropical plants (Vernonia amygdalina, Sida acuta, Ocimum gratissimum and Telfaria occidentalis) against beans weevil (Acanthscelides obtectus). International Journal of Physical Sciences,2010;5:753 792.
Riss T., Niles A., Moravec R., Karassina N. & Vidugiriene J.Cytotoxicity Assays: In Vitro Methods to Measure Dead Cells. Dans : Assay Guidance Manual [Internet]. Eli Lilly & Company and the National Center for Advancing Translational Sciences,2019.
Singh R., Bhardwaj P. & Sharma P.Antioxidant and toxicological evaluation of Cassia sopherain streptozotocin-induced diabetic Wistar rats. Pharmacognosy Research,2013;5(4):225 232 https://doi.org/10.4103/0974-8490.118767
Middleton E., Kandaswami C. & Theoharides T. C.The Effects of Plant Flavonoids on Mammalian Cells:Implications for Inflammation, Heart Disease, and Cancer. Pharmacological Reviews,2000;52(4):673 751.
Kahn R., Li W.-H., Martonchik J. V., Bruegge C. J., Diner D. J., Gaitley B. J., Abdou W., Dubovik O., Holben B., Smirnov A., Jin Z. & Clark D. MISR Calibration and Implications for Low-Light-Level Aerosol Retrieval over Dark Water, 2005. https://doi.org/10.1175/JAS3390.1
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