Sterculia setigera hydroethanolic extract protects brain tissues ex vivo against lipid peroxidation and possesses in vitro antioxidant and anti-inflammatory properties
Abstract
Medicinal plants remain good sources of remedies both in traditional and modern medicine. The present study aimed to evaluate ex vivo and in vitro neuroprotective effects of Sterculia setigera, a medicinal plant of Togolese flora. Inhibition of lipid peroxidation in rat brain homogenate was assayed ex vivo using the combination of ascorbic acid and FeCl2 as prooxidants. In vitro anti-inflammatory tests consisted in the evaluation of inhibition of albumin denaturation and red blood cells hemolysis. DPPH tests, FRAP test and Total antioxidant capacity (TAC) of the hydroethanolic extract of S. setigera were realized. Quantitative determination of total phenols, flavonoids and tannins was also performed. S. setigera hydroethanolic extract protected significantly brain homogenates against lipid peroxidation (-78.28 % of MDA reduction at 200 µg/mL, P<0.001). DDPH radical was scavenged by extract with IC50 of 21.25 ± 0.41 µg/mL, close to that of the standard ascorbic acid (20.60 ± 0.26 µg/mL). The TAC of S. setigera hydroethanolic extract expressed as equivalent of standard ascorbic acid was 102.44 ± 19.48 µg AAE/mg. The IC50 found for S. setigera (139.68 ± 0.36 µg/mL) when inhibiting RBCs hemolysis is very close to that of Diclofenac (128.41 ± 0.21 µg/mL). Among polyphenolic compounds quantified, flavonoids were well represented with concentration of 172.38 ± 8.36 µgRE/mg. The presence of these polyphenolics may explain good antioxidant and anti-inflammatory activities recorded, confirming its suitable usage in the local pharmacopoeia and making S. setigera a good candidate for neuroprotective drugs discovery.
Keywords: Sterculia setigera, neuroprotection, lipid peroxidation, antioxidant, anti-inflammatory.
Keywords:
Sterculia setigera, neuroprotection, lipid peroxidation, antioxidant, anti-inflammatoryDOI
https://doi.org/10.22270/jddt.v12i5-S.5643References
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