In vivo assessment of acute and subchronic toxicity of hydroethanolic extract of Ximenia americana L. (Olacaceae) stem bark
Abstract
Many studies have confirmed the traditional use of Ximenia americana (Olacaceae). Its different parts, leaf, fruit, roots, stem bark and root bark have showed pharmacological potentials. X. americana is a plant which possessed antibacterial, antidiabetic, antiviral and anticancer properties. The present study was undertaken to evaluate the oral acute and subchronic toxicity of hydroethanolic extract of X. americana stem bark on Sprague Dawley rats. In acute test, female rats were administrated once 5000 mg/kg and observed during 14 days for determination of toxicity signs. For the subchronic toxicity the extract (500 mg/kg and 1000 mg/kg) was administrated daily to both sexes of rats during 28 days. Control rats were administrated distilled water. Animals were observed, food and water consumption, mortality were monitoring during experiment period. Body weight, organs relative weight, hematological and biochemical parameters and histopathological changes were conducted at the end of treatment. Acute toxicity test revealed no adverse effects and no mortality. Extract administration during 28 days does not cause any behavioral change, change in body and organs relative weight and mortality. No significant variation was observed in hematological, biochemical parameters between treatment and control groups. No remarkable structural change was found in organs. However, triglycerides show significant variation in both sexes but this variation was not dose dependent. Results suggested that the LD50 of hydroethanolic extract of X. americana stem bark is higher than 5000 mg/kg and extract administration at 1000 mg/kg during 28 days does not cause toxicity in Sprague Dawley rats.
Keywords: Ximenia americana; stem bark; acute toxicity; subacute toxicity; hematological parameters; biochemical analysis.
Keywords:
Ximenia americana, stem bark, acute toxicity, subacute toxicity, hematological parameters, biochemical analysisDOI
https://doi.org/10.22270/jddt.v12i4.5567References
- Organisation Mondiale de la Santé (OMS) ; 2016. World report of diabetes.
- Njan, A. A., Olaoye, S. O., Afolabi, S. O., Ejimkonye, B. C., Soje, A., Olorundare, O. E., et al. Safety effect of fractions from methanolic leaf extract of Ocimum gratissimum on reproduction in male wistar rats Toxicology Reports 2019; 6:496-504. https://doi.org/10.1016/j.toxrep.2019.04.009
- Ekor, Martins. "The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety." Frontiers in pharmacology 2014; 4:177. https://doi.org/10.3389/fphar.2013.00177
- Ahmad L., He Y., Hao J.C., Semotiuk A., Liu Q.R., Mazari P., Toxic pyrrolizidine alkaloids provide a warning sign to overuse of the ethnomedicine Arnebia benthamii, J. Ethnopharmacol. 2018; 210:88–94. https://doi.org/10.1016/j.jep.2017.08.009
- Kale O.E., Awodele O. Akindele A.J., Subacute and subchronic oral toxicity assessments of Acridocarpus smeathmannii (DC.) Guill. & Perr. root in Wistar rats, Toxicol. Rep. 2019; 6:161–175. https://doi.org/10.1016/j.toxrep.2019.01.005
- Ugwah-Oguejiofor C.J., Okoli C.O., Ugwah M.O., Umaru M.L., Ogbulie C.S., Mshelia H.E., et al. Acute and sub-acute toxicity of aqueous extract of aerial parts of Caralluma dalzielii N. E. Brown in mice and rats, Heliyon .2019;5 (1):e01179. https://doi.org/10.1016/j.heliyon.2019.e01179
- Gupta, R., Polaka, S., Rajpoot, K., Tekade, M., Sharma, M. C., & Tekade, R. K. (2022). Importance of toxicity testing in drug discovery and research. In Pharmacokinetics and Toxicokinetic Considerations (pp. 117-144). Academic Press. https://doi.org/10.1016/B978-0-323-98367-9.00016-0
- Shagal, D. M. H., Kubmarawa, J., & Barminas, J. T. (2013). Evaluation of antimicrobial property of Ximenia americana. Journal of Biotechnology and pharmaceutical Research, 4(6):99-102.
- Agyigra, A. I., Ejiofor, J. I., Magaji, M. G., & Yakubu, Y. Evaluation of Methanol Stem-bark Extract of Ximenia americana Linn (Olacaceae) for Phytoconstituents and Gastroprotection in Rats. African Journal of Pharmacology and Therapeutics, 2017b 6(4):263-267.
- Aragao, T. P., Prazeres, L. D. K. T. D., Brito, S. A., Neto, P. J. R., Rolim, L. A., Almeida, J. R. G. D. S., ... & Wanderley, A. G. Contribution of secondary metabolites to the gastroprotective effect of aqueous extract of Ximenia americana L.(Olacaceae) stem bark in rats. Molecules, 2018; 23(1):112. https://doi.org/10.3390/molecules23010112
- Agustina, F., & Nugroho, R. P. P. Antibacterial Potential of Bidara Laut (Ximenia americana) Plant Against Vibrio alginolyticus and V. parahaemolyticus Bacteria. BIOEDUSCIENCE, 2021; 5(1), 15-23. https://doi.org/10.22236/j.bes/515091
- Maikai, V. A., Kobo, P. I., & Adaudi, A. O. Acute toxicity studies of aqueous stem bark extract of Ximenia americana. African Journal of Biotechnology, 2008; 7(10):1600-1603
- Agyigra, I. A., Ejiofor, J. I., & Magaji, M. G. Acute and subchronic toxicity evaluation of methanol stem-bark extract of Ximenia americana Linn (Olacaceae) in Wistar rats. Bulletin of Faculty of Pharmacy, Cairo University, 2017a; 55(2):263-267. https://doi.org/10.1016/j.bfopcu.2017.08.004
- OCDE. Guidelines for the Testing of Chemicals. OCDE 420. Acute Oral Toxicity Fixed Dose Procedure. Organization for Economic Cooperation and Development, 2001a; Paris, France.
- OCDE. Repeated dose oral toxicity test method. In: OCDE Guidelines for testing of chemicals, N° 407. Organization for Economic Cooperation and Development, 2008; Paris, France.
- Togbossi L A, Lawson-Evi P, Diallo A, Eklu-Gadegbeku K, Aklikokou K. Evaluation of antioxidant and antidepressant activity of hydro-alcoholic extract of Ximenia americana stem bark. J Phytopharmacol 2020; 9(5):323-328. doi: 10.31254/phyto.2020.9506
- Monte, F. J. Q., de Lemos, T. L. G., de Araújo, M. R. S., & de Sousa Gomes, E. Ximenia americana: chemistry, pharmacology and biological properties, a review. Phytochemicals–A Global Perspective of Their Role in Nutrition and Health, 2012; 429-450.
- Dantas, J. A., Ambiel, C. R., & Cuman, R. K. N. Reference values of some physiological parameters of rats from central vivarium of the State University of Maringa, Parana. Acta Sci Health Sci, 2006; 2:165-170.
- Porwal, M., Khan, N. A., & Maheshwari, K. K. Evaluation of acute and subacute oral toxicity induced by ethanolic extract of Marsdenia tenacissima leaves in experimental rats. Scientia Pharmaceutica, 2017; 85(3):29. https://doi.org/10.3390/scipharm85030029
- Lawson-Evi, P., Eklu-Gadegbeku, K., Agbonon, A., Aklikokou, K., Moukha, S., Creppy, E. E., et al.Toxicological assessment on extracts of Phyllanthus amarus Schum and Thonn. Scientific Research and Essays, 2008; 3(9):410-415. https://doi.org/10.5897/SRE.9000962
- Ateba S.B., Simo R.V., Mbanya J.C., Krenn L., Njamen D. Safety profile and gender specific differences of a methanol extract of Eriosema laurentii (Leguminosae) in acute and subchronic (28 days) oral toxicity studies in Wistar rats, Food Chem. Toxicol. 2014; 65:27–32. https://doi.org/10.1016/j.fct.2013.12.016
- Kadiene, E. U., Bialais, C., Ouddane, B., Hwang, J. S., & Souissi, S. Differences in lethal response between male and female calanoid copepods and life cycle traits to cadmium toxicity. Ecotoxicology, 2017; 26(9):1227-1239. https://doi.org/10.1007/s10646-017-1848-6
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