Therapeutic properties of aqueous extracts of leaves and stems bark of Prosopis africana (Guill. & Perr.) Taub. (Fabaceae) used in the management of dental caries

  • Alimata Bance Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso. 2. Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso
  • Seydou Sourabié Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso
  • Souleymane Compaoré Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso; Laboratory of Plant Biology and Ecology (LABEV), University Joseph KI-ZERBO, Burkina Faso
  • Eli Compaoré Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso. 2. Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso
  • W. Leila Marie Esther Belem-Kabre Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso
  • Vincent Ouedraogo Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso.
  • Ablassé Rouamba Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso.
  • Noufou Ouedraogo
  • Martin Kiendrebeogo Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso.

Abstract

Prosopis africana  (Guill. & Perr.) Taub. (Fabaceae) is used in the herbal medicine of Burkina Faso to treat dental caries. This study aims to contribute to the valorization of the said plant by investigating the antioxidant, anti-inflammatory and antibacterial properties of aqueous leaves and stems extracts.


The inhibitory activity on lipoxygenase was used to evaluate the anti-inflammatory effect of the extracts. The antioxidant activity of bots extracts of the plant was assessed using DPPH radical scavenging, ABTS+ radical cation decolorization. The anti-biofilm effect of the extracts was evaluated on Streptococcus mutans ATCC 25175, Staphylococcus aureus ATCC 43300, Pseudomonas aeruginosa PAOI and the anti-Quorum sensing effect on Chromobacterium CV026.


Aqueous extracts of Prosopis africana stems show the highest content of phenolic compounds (30,04± 0,59 mgAGE/100 mg extract) while those of the leaves show the highest content of total flavonoids (3.29 ± 0.53 mgQE/100mg extract). The aqueous extract of stem bark show the strongest antioxidant activity ( IC50 = 4.58±0.07µg/ml for the ABTS) , a best Inhibitory action on activity of lipoxygenase (IC50 = 13.42 ± 1.26 μg/mL ), a highest anti-biofilm activity ( 63.6%;  at the concentration of 100µg/ml) without affecting the bacterial growth. In addition, this extract has the strongest anti-quorum sensing activity with an percentage of inhibition 53,5%.


These findings suggested that the aqueous extracts of stem bark and leaves of Prosopis africana contain promoted phytomolecules to combat dental caries infections.


Keywords : Anti-biofilm, Anti-quorum sensing, Lipoxygenase, Prosopis africana

Keywords: Anti-biofilm, Anti-quorum sensing, Lipoxygenase, Prosopis africana

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Author Biographies

Alimata Bance, Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso. 2. Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso

Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso.

Seydou Sourabié, Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso

Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso.

Souleymane Compaoré, Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso; Laboratory of Plant Biology and Ecology (LABEV), University Joseph KI-ZERBO, Burkina Faso

Laboratory of Plant Biology and Ecology (LABEV), University Joseph KI-ZERBO, Burkina Faso.

Eli Compaoré, Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso. 2. Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso

1. Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso. 2. Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso

W. Leila Marie Esther Belem-Kabre, Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso

Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso

Vincent Ouedraogo, Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso.

Department of Traditional Medicine and Pharmacopoeia (MEPHATRA-PH), Institute for Research in Health Sciences (IRSS/CNRST), 03 P.O. Box 7034, Ouagadougou 03, Burkina Faso.

Ablassé Rouamba, Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso.

Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso.

Martin Kiendrebeogo, Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso.

Laboratory of Applied Biochemistry and Chemistry (LA.BIO.C.A), University Joseph KI-ZERBO,03 P.O. Box 7021, Ouagadougou 03, Burkina Faso.

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Bance A, Sourabié S, Compaoré S, Compaoré E, Belem-Kabre WLME, Ouedraogo V, Rouamba A, Ouedraogo N, Kiendrebeogo M. Therapeutic properties of aqueous extracts of leaves and stems bark of Prosopis africana (Guill. & Perr.) Taub. (Fabaceae) used in the management of dental caries. JDDT [Internet]. 15Nov.2021 [cited 1Dec.2021];11(6):108-14. Available from: https://jddtonline.info/index.php/jddt/article/view/5152