Acute toxicity and anti-inflammatory activity of an improved traditional medicine developed in Burkina Faso

Authors

  • Mohamed Bonewendé BELEMLILGA Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Tata Kadiatou TRAORÉ Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Gilchrist Abdoul Laurent Boly Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Séripouguin Rihanata BOURGOU Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Ladji TRAORE Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Salfo Ouédraogo Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Jean Claude R. P. OUEDRAOGO Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Sylvain ILBOUDO Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Moussa OUEDRAOGO Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), École Doctorale Sciences de la Santé (ED2S), Université Joseph KI-ZERBO, BP 7021, Burkina Faso
  • Dieudonné KINDA Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), École Doctorale Sciences de la Santé (ED2S), Université Joseph KI-ZERBO, BP 7021, Burkina Faso
  • Félix B. KINI Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Aristide TRAORE Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso
  • Noufou OUEDRAOGO Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Abstract

An Improved Traditional Medicine (ITM) in capsule form presented by traditional health practitioners in Burkina Faso as used for hepatoprotective and hepatocurative treatment for viral hepatitis B. The study's main objective was to investigate the anti-inflammatory properties of this ITM, which is used in treating viral hepatitis B in traditional medicine in Burkina Faso.  Phytochemical screening revealed the presence of flavonoids, saponins, tannins, coumarins, alkaloids, sterols and triterpenes. The in vitro anti-inflammatory assay inhibited LOX by 15.69 ± 0.50% at a 100 µg/mL concentration. The LD50 was estimated to be greater than 5000 mg/kg. In vivo activity using the carrageenan anti-oedema test showed good dose-dependent anti-inflammatory activity of ITM at 30, 65 and 130 mg/kg bw. After 3 hours, the extract at various doses reduced edema to over 50% inhibition. After 5 h, oedema was reduced to 85.209% at the 130 mg/kg. However, the reference acetylsalicylic acid (ASA) at 100 mg/kg bw showed a percentage of 85.048% at 5 h. The IC50 obtained after the ABTS, FRAP and LPO antioxidant tests of the phytomedicine were 351.00 µg/mL, 353.20 mM EAA/L and 81.13%. However, the IC50 with the DPPH method was higher than 104 mg/mL. The phytomedicine would have anti-inflammatory and antioxidant properties due to the presence of bioactive phytochemicals on inflammation and free radicals.

Keywords: ITM, Phytochemical, Acute toxicity, Anti-inflammatory, Antioxidants

Keywords:

ITM, Phytochemical, Acute toxicity, Anti-inflammatory, Antioxidants

DOI

https://doi.org/10.22270/jddt.v14i10.6812

Author Biographies

Mohamed Bonewendé BELEMLILGA, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

International Research Laboratory - Environnement, Santé, Sociétés (IRL 3189, ESS) CNRS/CNRST/UCAD/UGB/USTTB, Burkina Faso.

Tata Kadiatou TRAORÉ, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Gilchrist Abdoul Laurent Boly, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Séripouguin Rihanata BOURGOU, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), École Doctorale Sciences de la Santé (ED2S), Université Joseph KI-ZERBO, BP 7021, Burkina Faso

Ladji TRAORE, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), École Doctorale Sciences de la Santé (ED2S), Université Joseph KI-ZERBO, BP 7021, Burkina Faso

Salfo Ouédraogo, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Jean Claude R. P. OUEDRAOGO, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Sylvain ILBOUDO, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

International Research Laboratory - Environnement, Santé, Sociétés (IRL 3189, ESS) CNRS/CNRST/UCAD/UGB/USTTB, Burkina Faso.

Moussa OUEDRAOGO, Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), École Doctorale Sciences de la Santé (ED2S), Université Joseph KI-ZERBO, BP 7021, Burkina Faso

Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), École Doctorale Sciences de la Santé (ED2S), Université Joseph KI-ZERBO, BP 7021, Burkina Faso

Dieudonné KINDA, Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), École Doctorale Sciences de la Santé (ED2S), Université Joseph KI-ZERBO, BP 7021, Burkina Faso

Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), École Doctorale Sciences de la Santé (ED2S), Université Joseph KI-ZERBO, BP 7021, Burkina Faso

Félix B. KINI, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Aristide TRAORE, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

Noufou OUEDRAOGO, Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST) BP 7047 Ouagadougou 03, Burkina Faso

International Research Laboratory - Environnement, Santé, Sociétés (IRL 3189, ESS) CNRS/CNRST/UCAD/UGB/USTTB, Burkina Faso.

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15-10-2024
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1.
BELEMLILGA MB, TRAORÉ TK, Boly GAL, BOURGOU SR, TRAORE L, Ouédraogo S, et al. Acute toxicity and anti-inflammatory activity of an improved traditional medicine developed in Burkina Faso. J. Drug Delivery Ther. [Internet]. 2024 Oct. 15 [cited 2025 Jan. 21];14(10):31-4. Available from: https://jddtonline.info/index.php/jddt/article/view/6812

How to Cite

1.
BELEMLILGA MB, TRAORÉ TK, Boly GAL, BOURGOU SR, TRAORE L, Ouédraogo S, et al. Acute toxicity and anti-inflammatory activity of an improved traditional medicine developed in Burkina Faso. J. Drug Delivery Ther. [Internet]. 2024 Oct. 15 [cited 2025 Jan. 21];14(10):31-4. Available from: https://jddtonline.info/index.php/jddt/article/view/6812

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