Acute toxicity and anti-inflammatory activity of an improved traditional medicine developed in Burkina Faso
Abstract
An Improved Traditional Medicine (ITM) in capsule form presented by traditional health practitioners in Burkina Faso as used for hepatoprotective and hepatocurative treatment for viral hepatitis B. The study's main objective was to investigate the anti-inflammatory properties of this ITM, which is used in treating viral hepatitis B in traditional medicine in Burkina Faso. Phytochemical screening revealed the presence of flavonoids, saponins, tannins, coumarins, alkaloids, sterols and triterpenes. The in vitro anti-inflammatory assay inhibited LOX by 15.69 ± 0.50% at a 100 µg/mL concentration. The LD50 was estimated to be greater than 5000 mg/kg. In vivo activity using the carrageenan anti-oedema test showed good dose-dependent anti-inflammatory activity of ITM at 30, 65 and 130 mg/kg bw. After 3 hours, the extract at various doses reduced edema to over 50% inhibition. After 5 h, oedema was reduced to 85.209% at the 130 mg/kg. However, the reference acetylsalicylic acid (ASA) at 100 mg/kg bw showed a percentage of 85.048% at 5 h. The IC50 obtained after the ABTS, FRAP and LPO antioxidant tests of the phytomedicine were 351.00 µg/mL, 353.20 mM EAA/L and 81.13%. However, the IC50 with the DPPH method was higher than 104 mg/mL. The phytomedicine would have anti-inflammatory and antioxidant properties due to the presence of bioactive phytochemicals on inflammation and free radicals.
Keywords: ITM, Phytochemical, Acute toxicity, Anti-inflammatory, Antioxidants
Keywords:
ITM, Phytochemical, Acute toxicity, Anti-inflammatory, AntioxidantsDOI
https://doi.org/10.22270/jddt.v14i10.6812References
Terrault NA, Bzowej NH, Chang KM, Hwang JP, Jonas MM, Murad MH. AASLD guidelines for treatment of chronic hepatitis B. Hepatology. 2016;63(1):261-283. https://doi.org/10.1002/hep.28156
Farber JL. Mechanisms of cell injury by activated oxygen species. Environ Health Perspect. 1994;102(SUPPL. 10):17-24. https://doi.org/10.1289/ehp.94102s1017
CROSS CE. Oxygen Radicals and Human Disease. Ann Intern Med. 1987;107(4):526. https://doi.org/10.7326/0003-4819-107-4-526
Kulkarni A, Farooqui A, Hoppensteadt D, Van Thiel D, Siddiqui F, Fareed J. Biomarkers, inflammation, and thrombosis in Hepatocellular Carcinoma. FASEB J. May 14, 2021;35(S1):fasebj.2021.35.S1.03754. https://doi.org/10.1096/fasebj.2021.35.S1.03754
Arulselvan P, Fard MT, Tan WS, Gothai S, Fakurazi S, Norhaizan ME, et al. Role of Antioxidants and Natural Products in Inflammation. Oxid Med Cell Longev. 2016;2016. https://doi.org/10.1155/2016/5276130
Guyton and Hall. Textbook Medical Physiology, Twelfth ed. Schmitt W, Gruliow R (eds): Saunders elsevier 2011.
Sun Q, Zhu J, Cao F, Chen F. Anti-inflammatory properties of extracts from Chimonanthus nitens Oliv. leaf. Bach H (ed): PLoS One. July 10, 2017;12(7):1 https://doi.org/10.1371/journal.pone.0181094
Monteiro B, Steagall P V. Antiinflammatory Drugs. Vet Clin North Am - Small Anim Pract. November 1, 2019 [cited June 20, 2023];49(6):993-1011. https://doi.org/10.1016/j.cvsm.2019.07.009
Dunnill C, Patton T, Brennan J, Barrett J, Dryden M, Cooke J, et al. Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS-modulating technologies for augmentation of the healing process. Int Wound J. 2017;14(1):89-96. https://doi.org/10.1111/iwj.12557
Moasser E, Azarpira N, Ghorbani dalini A, Shirazi B. Paraoxonase 1 (PON1) gene polymorphism and haplotype analysis in type 2 diabetes mellitus: a case-control study in the south Iranian population. Int J Diabetes Dev Ctries. 2018;38(1):62-68. https://doi.org/10.1007/s13410-016-0544-z
Gulumian M, Yahaya ES, Steenkamp V. African Herbal Remedies with Antioxidant Activity: A Potential Resource Base for Wound Treatment. Evidence-based Complement Altern Med. 2018;2018. https://doi.org/10.1155/2018/4089541
Dobrian AD, Lieb DC, Cole BK, Taylor-Fishwick DA, Chakrabarti SK, Nadler JL. Functional and pathological roles of the 12- and 15-lipoxygenases. Prog Lipid Res. 2011;50(1):115-131. https://doi.org/10.1016/j.plipres.2010.10.005
Hoesel B, Schmid JA. The complexity of NF-κB signaling in inflammation and cancer. Mol Cancer. 2013;12(86):705-716. https://doi.org/10.1186/1476-4598-12-86
Nagar D, Katare V, Sen S, Delouri A, Nema S. Evaluation of Antioxidant Activity of Rosa indica Linn . Asian Journal of Dental and Health Sciences. 2023;3(1):5-7. https://doi.org/10.22270/ajdhs.v3i1.34
Speakman JR, Selman C. The free-radical damage theory: Accumulating evidence against a simple link of oxidative stress to ageing and lifespan. BioEssays. 2011;33(4):255-259. https://doi.org/10.1002/bies.201000132
Rana J, Rana J, Prakash V, Sagar A. Status of Indian Medicinal Plants in Terms of Antioxidant Activity (in Vitro). Plant Arch. 2021;21(No 1):722-763. https://doi.org/10.51470/PLANTARCHIVES.2021.v21.no1.100
Verma N, Katare V, Kalme N, Delouri A, Nema S, Evaluation of Antioxidant Activity of Trigonella foenum graecum Seeds Extract, Asian Journal of Dental and Health Sciences, 2023;3(1):11-14. https://doi.org/10.22270/ajdhs.v3i1.33
Taylor JLS, Rabe T, Mcgaw LJ, Jäger AK, Van Staden J. Towards the scientific validation of traditional medicinal plants. Plant Growth Regul. 2001;34(1):23-37. https://doi.org/10.1023/A:1013310809275
Bernstein N, Akram M, Daniyal M, Koltai H, Fridlender M, Gorelick J. Antiinflammatory Potential of Medicinal Plants: A Source for Therapeutic Secondary Metabolites [Internet], 1st edition. Vol. 150, Advances in Agronomy. Elsevier Inc. 2018. https://doi.org/10.1016/bs.agron.2018.02.003
Boly R, Compaore S, Ouedraogo S, Zeba M, Magnini DR, Bance A, et al. Collaboration between practitioners of traditional and conventional medicine: A report of an intervention carried out with traditional women healers in the province of Sanmatenga (Burkina Faso) to improve the obtaining of the license to practice tradition. Int NGO J. 2021;16(1):9-16. https://doi.org/10.5897/INGOJ2021.0355
Jato J, Orman E, Duah Boakye Y, Oppong Bekoe E, Oppong Bekoe S, Asare-Nkansah S, et al. Anthelmintic Agents from African Medicinal Plants: Review and Prospects. Evidence-Based Complement Altern Med. 2022;2022. https://doi.org/10.1155/2022/8023866
Silué GNA, Belemlilga MB, Djadji ATL, Traoré KT, Boly GAL, Koala M, et al. Anti-inflammatory and antioxidant activities assessment of an aqueous extract of Ceiba pentandra (L.) Gaertn (Malvaceae) . RPS Pharm Pharmacol Reports. 2023;2(4):1-8. https://doi.org/10.1093/rpsppr/rqad037
Tibiri André, Boria Sébastien, Traoré KadiatouTata, Ouédraogo Noufou, Nikièma Aude, Ganaba Souleymane, et al. Gestion des maladies hépatiques et plantes utilisées par les tradipraticiens au Burkina Faso. In Profizi Jean-Pierre, Ardila-Chauvet Stéphanie, Billot Claire, et al. (eds): Biodiversité des écosystèmes intertropicaux IRD Éditions. 2022; 2011-2024. https://doi.org/10.4000/books.irdeditions.41287
Li Y, Guo B, Wang W, Li L, Cao L, Yang C, et al. Characterization of phenolic compounds from Phyllanthus emblica fruits using HPLC-ESI-TOF-MS as affected by an optimized microwave-assisted extraction. Int J Food Prop. 2019;22(1):330-342. https://doi.org/10.1080/10942912.2019.1583249
Kashyap AK, Bisoyi P, Jain BP. Antioxidant, Anti-Microbial, Anti-Inflammatory and Cytotoxic Effect of Macrotyloma Uniflorum Leaf Extract. Adv Pharmacol Pharm. 2023;11(3):179-186. https://doi.org/10.13189/app.2023.110301
Arora V, Rani L, Grewal AS, Dureja H. Natural product-based antiinflammatory agents. In Recent Developments in Anti-Inflammatory Therapy Academic Press 2023; 183-232. https://doi.org/10.1016/B978-0-323-99988-5.00011-5
Gopa B, Bhatt J, Hemavathi KG. A comparative clinical study of hypolipidemic efficacy of Amla (Emblica officinalis) with 3-hydroxy-3-methylglutaryl-coenzyme-A reductase inhibitor simvastatin. Indian J Pharmacol. 2012;44(2):238-242. https://doi.org/10.4103/0253-7613.93857
Traore TK, Andre T, Noufou O, Ernest SN, Yhi-pene NJ, Pierre GI. Ethnopharmacological plants used to treat hepatitis and their anti-oxidant activity of district of Bobo-Dioulasso (Burkina Faso). Int J Pharmacol Res. 2018;8(3):15. https://doi.org/10.7439/ijpr.v8i3.4643
Anees Ali Jafri S, Mehmood Khalid Z, Rizwan Khan M, Ashraf S, Ahmad N, Mahmoud Karami A, et al. Evaluation of some essential traditional medicinal plants for their potential free scavenging and antioxidant properties. J King Saud Univ - Sci. 2023;35(3):102562. https://doi.org/10.1016/j.jksus.2023.102562
Bekro Y-A, Mamyrbekova J, Boua B, Tra Bi F, Ehile E. Étude ethnobotanique et screening phytochimique de Caesalpinia benthamiana (Baill.) Herend. et Zarucchi (Caesalpiniaceae). Sci Nat. 2008;4(2):217-225. https://doi.org/10.4314/scinat.v4i2.42146
May JC, Wheeler RM, Grim E. The gravimetric method for the determination of residual moisture in freeze-dried biological products. Cryobiology. 1989;26(3):277-284. https://doi.org/10.1016/0011-2240(89)90023-0
Ciulei I. Methodology for analysis of vegetable drugs. Practical manual on the industrial utilisation of medicinal and aromatic plants. Ministry of chemical industry B (ed): Bucharest,. 1982.
Wagner H, Bladt S. Plant Drug Analysis, Second. Springer (ed): 1996. https://doi.org/10.1007/978-3-642-00574-9
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods Enzymol. 1999;299(1974):152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
Kumaran A, Joel Karunakaran R. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. Lwt. 2007;40(2):344-352. https://doi.org/10.1016/j.lwt.2005.09.011
Abdel-Hameed ESS. Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples. Food Chem. 2009;114(4):1271-1277. https://doi.org/10.1016/j.foodchem.2008.11.005
OECD. Test No. 423: Acute Oral toxicity - Acute Toxic Class Method. Oecd Guidel Test Chem. February 8, 2002 [cited September 30, 2021];(December):1-14.
Ouedraogo GG, Ouedraogo M, Lamien-sanou A, Lompo M, Olga M, Guissou PI. Acute and Subchronic Toxicity Studies of Roots Barks Extracts of Calotropis procera ( Ait .) R . Br Used in the Treatment of Sickle Cell Disease in Burkina Faso. 2013;4(5):194-200. https://doi.org/10.19026/bjpt.4.5401
Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw. Exp Biol Med. 1962;3(111):544-547. https://doi.org/10.3181/00379727-111-27849
Malterud KE, Rydland KM. Inhibitors of 15-lipoxygenase from orange peel. J Agric Food Chem. 2000;48(11):5576-5580. https://doi.org/10.1021/jf000613v
Lompo M, Traore R, Ouedraogo N, Kini F, Tibiri A, Guissou IP, et al. In vitro antioxidant activity and phenolic contents of different fractions of ethanolic extract from Khaya senegalensis A. Juss. (Meliaceae) stem barks. African J Pharm Pharmacol. 2016;10(23):501-507. https://doi.org/10.5897/AJPP2016.4564
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. May 1999;26(9-10):1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Hinneburg I, Damien Dorman HJ, Hiltunen R. Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem. 2006;97(1):122-129. https://doi.org/10.1016/j.foodchem.2005.03.028
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-358. https://doi.org/10.1016/0003-2697(79)90738-3
Raja KS, Taip FS, Azmi MMZ, Shishir MRI. Effect of pre-treatment and different drying methods on the physicochemical properties of Carica papaya L. leaf powder. J Saudi Soc Agric Sci. 2019;18(2):150-156. https://doi.org/10.1016/j.jssas.2017.04.001
Daniel Azubuike A. Comparative Chemical Constituents of some Cassia Species and their Pharmacognistic Importance in South Eastern Nigeria. Sci J Chem. 2015;3(3):40. https://doi.org/10.11648/j.sjc.20150303.12
Musa AA. Cytotoxicity activity and phytochemical screening of Cochlospermum tinctorium perr ex A. Rich rhizome. J Appl Pharm Sci. 2012;2(7):155-159. https://doi.org/10.7324/JAPS.2012.2723
Küpeli E, Yesilada E. Flavonoids with anti-inflammatory and antinociceptive activity from Cistus laurifolius L. leaves through bioassay-guided procedures. J Ethnopharmacol. 2007;112(3):524-530. https://doi.org/10.1016/j.jep.2007.04.011
Sdayria J, Rjeibi I, Feriani A, Ncib S, Bouguerra W, Hfaiedh N, et al. Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice. Pain Res Manag. July 4, 2018. https://doi.org/10.1155/2018/4838413
Gillessen A, Schmidt HHJ. Silymarin as Supportive Treatment in Liver Diseases: A Narrative Review. Adv Ther. 2020. https://doi.org/10.1007/s12325-020-01251-y
Kharchoufa L, Bouhrim M, Bencheikh N, El Assri S, Amirou A, Yamani A, et al. Acute and Subacute Toxicity Studies of the Aqueous Extract from Haloxylon scoparium Pomel (Hammada scoparia (Pomel)) by Oral Administration in Rodents. Biomed Res Int. 2020. https://doi.org/10.1155/2020/4020647
United Nations. Globally Harmonized System of classification and labelling of chemicals (GHS), tenth revised edition united [Internet]. New York and Geneva: United Nations 2023. Available at: ST/SG/AC.10/30/Rev.10
Di Rosa M, Giroud JP, Willoughby DA. Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. J Pathol. 1971;104(1):15-29. https://doi.org/10.1002/path.1711040103
Andersson J. The inflammatory reflex - Introduction. J Intern Med. 2005;257(2):122-125. https://doi.org/10.1111/j.1365-2796.2004.01440.x
Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci. 2004;96(3):229-245. https://doi.org/10.1254/jphs.CRJ04003X
Sy GY, Fall AD, Diatta W, Gueye M, Badji K, Bassène E, et al. Analgesic and anti-inflammatory activity of aqueous root extract of Cassia sieberiana D. C. (Caesalpiniaceae). African J Pharm Pharmacol. 2009;3(12):651-653.
Ahmed TS, Magaji MG, Yaro AH, Musa AM, Adamu AK. Aqueous methanol extracts of Cochlospermum tinctorium (A. Rich) possess analgesic and anti-inflammatory activities. J Young Pharm. 2011;3(3):237-242. https://doi.org/10.4103/0975-1483.83774
Diarra A, Agossa K, Youl ENH. The Potential of Cochlospermum tinctorium, Flueggea virosa, and Waltheria indica Traditional Plants From Burkina Faso in Treating Periodontitis: A Systematic Review. Cureus. 2024;16(1):1-10. https://doi.org/10.7759/cureus.52471
Macedo T, Ferreres F, Pereira DM, Oliveira AP, Gomes NGM, Gil-Izquierdo Á, et al. Cassia sieberiana DC. leaves modulate LPS-induced inflammatory response in THP-1 cells and inhibit eicosanoid-metabolizing enzymes. J Ethnopharmacol. 2021;269 (December 2020). https://doi.org/10.1016/j.jep.2020.113746
Saleh HA, Yousef MH, Abdelnaser A. The Anti-Inflammatory Properties of Phytochemicals and Their Effects on Epigenetic Mechanisms Involved in TLR4/NF-κB-Mediated Inflammation. Front Immunol. 2021;12(March):1-29. https://doi.org/10.3389/fimmu.2021.606069
Maheswari U, Sridevi Sangeetha KS, Umamaheswari S, Uma C, Reddy M, Kalkura SN. Flavonoids: therapeutic potential of natural pharmacological agents. Int J Pharm Sci Res. 2016;7(10):3924. Available at: http://dx.doi.org/10.13040/IJPSR.0975-8232.7
Kempuraj D, Madhappan B, Christodoulou S, Boucher W, Cao J, Papadopoulou N, et al. Flavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells. Br J Pharmacol. 2005;145(7):934-944. https://doi.org/10.1038/sj.bjp.0706246
Belemlilga MB, Moyenga F, Boly AGL, Ouédraogo H, Compaoré S, Yoda J, et al. Phytochemical and in vitro anthelmintic properties of mesocarp of fruit extracts from Balanites aegyptiaca (L.) Delile (Zygophyllaceae). J Herbmed Pharmacol. January 1, 2024 [cited February 20, 2024];13(1):144-152. Available at: http://herbmedpharmacol.com/Article/jhp-48247 https://doi.org/10.34172/jhp.2024.48247
Villanueva-Bermejo D, Vázquez E, Villalva M, Santoyo S, Fornari T, Reglero G, et al. Simultaneous supercritical fluid extraction of heather (Calluna vulgaris L.) and marigold (Calendula officinalis L.) and anti-inflammatory activity of the extracts. Appl Sci. 2019;9(11). https://doi.org/10.3390/app9112245
Chel-Guerrero LD, Castañeda-Corral G, López-Castillo M, Scampicchio M, Morozova K, Oney-Montalvo JE, et al. In vivo Anti-Inflammatory Effect, Antioxidant Activity, and Polyphenolic Content of Extracts from Capsicum chinense By-Products. Molecules. 2022;27(4). https://doi.org/10.3390/molecules27041323
Klotoé JR, Dossa AK, Agbodjento E, Dougnon V, Ohouko HF, Afoussatou A, et al. Effect of the association of Chromolaena odorata or Combretum micranthum on the antioxidant activity of Cochlospermum tinctorium. Rev RAMReS - Série Pharm Méd Trad Afr. 2021;20(2):39-53.
Zongo E, Busuioc A, Meda RNT, Botezatu AV, Mihaila MD, Mocanu AM, et al. Exploration of the Antioxidant and Anti-inflammatory Potential of Cassia sieberiana DC and Piliostigma thonningii (Schumach.) Milne-Redh, Traditionally Used in the Treatment of Hepatitis in the Hauts-Bassins Region of Burkina Faso. Pharmaceuticals. 2023;16(1). https://doi.org/10.3390/ph16010133
Ghasemzadeh A, Jaafar HZE, Rahmat A, Wahab PEM, Halim MRA. Effect of different light intensities on total phenolics and flavonoids synthesis and anti-oxidant activities in young ginger varieties (Zingiber officinale Roscoe). Int J Mol Sci. 2010;11(10):3885-3897. https://doi.org/10.3390/ijms11103885
Edeas M. Les polyphénols et les polyphénols de thé. Phytotherapie. 2007;5(5):264-270. https://doi.org/10.1007/s10298-007-0268-9
Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R, Gnanadhas EN, Lakshminarasaiah U, et al. Antioxidants and human diseases. Clin Chim Acta. 2014;436:332-347. https://doi.org/10.1016/j.cca.2014.06.004
Noufou O, Sawadogo W, Tibiri A, Lompo M, Dijoux M, Diederich M, et al. Phenolic Contents and In vitro Pharmacological Activities of Methanolic Extract of Pterocarpus erinaceus Poir. Stem Bark (Fabaceae). Br J Pharm Res. 2016;10(2):1-7. https://doi.org/10.9734/BJPR/2016/23149
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