New Smartphone Based Colorimetric Method Development and Validation of Emtricitabine in Bulk and Tablet Dosage Form
Abstract
The novel smartphone based colorimetric application called PhotoMetrix-PRO has been used for quantitative analysis of Emtricitabine in bulk and tablet dosage form and it compared with conventional UV –visible spectrophtotometry. Smartphone based colorimetric method and UV method was based on detection of intensity of color with increasing concentration Ammonium metavanadate (inorganic oxidizing agent) which was used for preparation Mandeline reagent they used as coloring agent for colour development .The reagent is orange red colour but when it react with Emtricitabine it convert into green color complex. The developed methods have good linearity 50-250μg/ml. The color intensity of API increase with increasing concentration. All images were captured through mobile phone and analysed in PhotoMetrix PRO application. This application convert image into Red, Green and Blue(RGB)histogram and regression models were built into PhotoMetrix-PRO Application both the method have correlation coefficient R2>0.995.The LOD and LOQ for PhotoMetrix PRO 4.77μg/ml and14.92μg/ml and UV-visible spectrophotometry method 4.99 μg/ml and 14.96 μg/ml respectively. The % RSD of Emtricitabine by PhotoMetrix PRO and UV method was 99% (%RSD <2). Appling statistical tool i.e paired two test on both the methods result indicate that both methods are equally significant.
Keywords: Smartphone, PhotoMetrix PRO, UV Spectrophotometry, RGB
Keywords:
Smartphone, PhotoMetrix PRO, UV Spectrophotometry, RGBDOI
https://doi.org/10.22270/jddt.v11i4.4933References
Kılıç V, Alankus G, Horzum N, Ali YMutlu, Bayram A, Solmaz ME. Single-Image-Referenced Colorimetric Water Quality Detection Using a Smartphone. ACS Omega. 2018; 3(5):5531–6. DOI: https://doi.org/10.1021/acsomega.8b00625.
Costa AB da, Helfer GA, Barbosa JLV, Teixeira, ID, Santos RO, Santos RB dos, et al. PhotoMetrix UVC: A New Smartphone-Based Device for Digital Image Colorimetric Analysis Using PLS Regression. J Braz Chem Soc. 2021; 32(3):675–83. https://doi.org/10.21577/0103-5053.20200199.
Helfer GA, Magnus VS, Böck FC, Teichmann A, Ferrão MF, Cost AB da. PhotoMetrix: An Application for Univariate Calibration and Principal Components Analysis Using Colorimetry on Mobile Devices. J Braz Chem Soc. 2016; 1–8. https://doi.org/10.5935/0103-5053.20160182.
Sowjanya G, Kommoju M. Colorimetric Approaches To Drug Analysis And Applications -A Review. 2019; 9(1). https://doi.org/10.46624/ajptr.2019.v9.i1.002
jaswant dunna, k.vijetha, B Geeta Priyadarshini, T Dileep Kumar. Development and Validation of a Novel Colorimetric Method for the Estimation of Emtricitabine in Bulk and Tablet Formulation. Indian Journal of Pharmaceutical Sciences. 2016. https://doi.org/10.4172/pharmaceutical-sciences.1000183
Singh G, Pai RS. High-Performance Liquid Chromatographic Method for Analysis of Emtricitabine in Rat Plasma: Method Development, Validation and Application to a Pharmacokinetic Study. International Scholarly Research Notices. 2013. https://doi.org/10.1155/2013/329072
Runja C, Kumar PR, Avanapu SR. A Validated Stability Indicating RP-HPLC Method for the Determination of Emtricitabine, Tenofovir Disoproxil Fumarate, Elvitegravir and Cobicistat in Pharmaceutical Dosage Form. J Chromatogr Sci. 2016 May-Jun; 54(5):759-64. DOI: 10.1093/chromsci/bmw004
Kokkirala TK, Suryakala D. RP-HPLC method development and validation for the estimation of Emtricitabine, Bictegravir and Tenofovir alafenamide in bulk and pharmaceutical dosage form. Journal of Taibah University for Science. 2019; 13(1):1137–46. DOI: 10.1080/16583655.2019.1689601
Venkatesan S, Kannappan N. Simultaneous Spectrophotometric Method for Determination of Emtricitabine and Tenofovir Disoproxil Fumarate in Three-Component Tablet Formulation Containing Rilpivirine Hydrochloride. International Scholarly Research Notices. 2014; 8. https://doi.org/10.1155/2014/541727
Choudhari VP, Ingale S, Gite SR, Tajane DD, Modak VG, Ambekar A. Spectrophotometric simultaneous determination of Tenofovir disoproxil fumarate and Emtricitabine in combined tablet dosage form by ratio derivative, first order derivative and absorbance corrected methods and its application to dissolution study. Pharm Methods. 2011; 2(1):47-52. doi:10.4103/2229-4708.81096
Hay MH, Azza A. Gazy, Rasha A. Shaalan, and Heba K. Ashour, Simple Spectrophotometric Methods for Determination of Tenofovir Fumarate and Emtricitabine in Bulk Powder and in Tablets. Journal of Spectroscopy. 2013:1-7. https://doi.org/10.1155/2013/937409
Joshi M, Nikalje AP, Shahed M, Dehghan M. HPTLC method for the simultaneous estimation of emtricitabine and tenofovir in tablet dosage form. Indian journal of pharmaceutical sciences. 2009 Jan; 71(1):95. https://doi.org/10.4103/0250-474X.51951
Published
Abstract Display: 823 
PDF Downloads: 765 PDF Downloads: 113 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.