Protection of histomorphology of vital organs by methanolic seed extract of Nigella sativa against cadmium-induced tissue injuries in rats
Abstract
Background: Chemical-induced organ injuries have been on a fast rise for decades and these injuries have become common causes of mortality and morbidity in the society. Edible plant materials with medicinal properties have been used for treating various diseases for many centuries in folk medicine. Recently, the role of food or medicinal plants in human health has received considerable attention. Traditional uses of N. sativa seed range from soothing wounds to remedying cough, eczema, diabetes, inflammation of the bronchi and tooth aches; and these point to substantial tissue effects.
Objective: We investigated the protective effects of methanolic seed extract of Nigella sativa (MENS) against cadmium-induced histomorphological alterations in heart, kidney and liver tissues of albino rats.
Methods: Twenty five (25) male albino rats, weighing (200±20g), were randomly grouped into five groups: A, B, C, D, and E. Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), and group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 14 days. Group A (Normal control) received no administration. Heart, kidney and liver were harvested for histopathological analyses.
Results: Cadmium (CdCl2) induced significant histomorphological changes in the studied organs, and the heart was the most damaged of all the organs studied; however a significantly ameliorative effect by methanolic seed extracts was observed.
Conclusion: Nigella sativa seed extract is potentially tissue-protective against harmful chemical toxins like cadmium.
Keywords:
anticardiotoxic, cadmium, Nigella sativa, medicinal food, antinephrotoxic, ethnopharmacology, antihepatotoxicDOI
https://doi.org/10.22270/jddt.v11i3-S.4822References
Hosseini M, Mohammadpour T, Karami R, Rajaei Z, Sadeghnia H R, Soukhtanloo M. Effects of the hydro-alcoholic extract of Nigella sativa on scopolamine-induced spatial memory impairment in rats and its possible mechanism. Chinese Journal of Integrative Medicine 2015; 21(6):438-444. https://doi.org/10.1007/s11655-014-1742-5
Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA, Anwar F. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Journal of Tropical Biomedicine 2013; 3(5):337-352. https://doi.org/10.1016/S2221-1691(13)60075-1
Amin, B, Hosseinzadeh H. Black cumin (Nigella sativa) and its active constituent, thymoquinone: an overview on the analgesic and anti-inflammatory effects. Planta Medica 2015; 82: 8-16. https://doi.org/10.1055/s-0035-1557838
Darakhshan S, Pour AB, Colagar AH, Sisakhtnezhad S. Thymoquinone and its therapeutic potentials. Pharmacoogical Reseasrch 2015; 95-96: 138-158. https://doi.org/10.1016/j.phrs.2015.03.011
Rafati R, Kazemi S, Moghadamnia A. Cadmium toxicity and treatment: An update. Caspian Journal internal medicine 2017; 8(3):135-145.
Yu C,, Ling Q, Yan S, Jing L, Zhongda C, Zhenyu P. Cadmium contamination in various environmental materials in an industrial area, Hangzhou, China. Chemical Speciation and Bioavailability 2010; 22(1):35-42. https://doi.org/10.3184/095422910X12631439471494
Sharma H, Blessy NR, Mathew B. The characteristics, toxicity and effects of cadmium. International Journal of Nanoscience and Nanotechnology 2015; 3:1-9.
Bernhoft RA. Cadmium toxicity and treatment. The Scientific World Journal 2013; 2013: 394652. https://doi.org/10.1155/2013/394652
Wallin M, Sallstern G, Lundh T, Barregard L. Low level Cadmium exposure and effects on kidney function. Occupational and environmental medicine 2014; 71(12):848-854. https://doi.org/10.1136/oemed-2014-102279
Shen J, Wang X, Zhon D, Li T, Tang L, Gong T, Su J, Liang P. Modelling cadmium-induced cardiotoxicity using human pluripotent stem cell-derived cardiomyocetes. Journal of Cellular and Molecular Medicine 2018; 22(9):4221- 4235. https://doi.org/10.1111/jcmm.13702
Borné Y, Barregard L, Persson M, Hedblad B, Fagerberg B, Engstrom G. Cadmium exposure and incidence of heart failure and atrial fibrillation: a population-based prospective cohort study. British Medical Journal open 2015; 5(6):e007366. https://doi.org/10.1136/bmjopen-2014-007366
Hyder O, Chung M, Cosgrove D, Herman JM, Li Z, Firoozmand A, Gurakar A, Koteish A, Pawlik TM. Cadmium exposure and liver disease among US adults. Journal of Gastrointestinal Surgery 2013; 17(7):1265-1273. https://doi.org/10.1007/s11605-013-2210-9
Ajibesin KK. Dacryodes edulis (G. Don) HJ. Lam: A review on its medicinal, phytochemical and economic properties. Research Journal of Medicinal Plant 2011; 5(1):32-41. https://doi.org/10.3923/rjmp.2011.32.41
Babaei HA, Motamedifar M, Khalifat S, Mohammadi A, Khosrow Z, Motamedifar A. In vitro study of antibacterial property and cytotoxic effects of aqueous, ethanolic, methanolic, and hydroalcoholic extracts of fenugreek seed. Pakistan Journal of Medical and Health Sciences 2018; 12(2):906-910.
Lorke D. A new approach to practical acute toxicity testing. Archives of Toxicology 1983; 54(4):275-287. https://doi.org/10.1007/BF01234480
Trease G, Evans SM. Pharmacognosy: (15th Edition). English Language Book Society. Bailliere Tindall, London, 2002. p. 23-67.
Baker FJ, Silverton RE, Pallister CJ Baker and Silverton's Introduction to Laboratory Technology. 7th Edition, Butterworth-Heinemann, Wobrun, MA, USA, 1998; page 448.
Gurcan MN, Boucheron L, Can A, Madabhushi A, Rajpoot N, Yener B. Histopathological image analysis: A review. IEEE Review Biomedical Engineering 2009; 2:147-171. https://doi.org/10.1109/RBME.2009.2034865
Okamoto R, Ali Y, Hashizume R, Suzuki N, Ito M. BNP as a major player in the heart-kidney connection. International Journal of Molecular Sciences 2019; 20(14):e3581. https://doi.org/10.3390/ijms20143581
Rangaswami J, Bhalla V, Blair EA, Chang TI, Costa S, Mezue K. et al. Cardiorenal syndrome: Classification, pathophysiology, Diagnosis, and Treatment strategies: A scientific statement from the American Heart Association. Circulation. 2019; 139:e840-878. https://doi.org/10.1161/CIR.0000000000000664
Ding HR, Wang JL, Ren HZ, Shi XL. Lipometabolism and glycometabolism in liver diseases. BioMed Res Int. 2018; 2018: ID 1287127, 7 pages. https://doi.org/10.1155/2018/1287127
Bücker-Neto L, Paiva ALS, Machado RD, Arenhart RA, Margis- Pinheiro M. Interaction between plant hormones and heavy metals responses. Genetics and Molecular Biology 2017; 40(1):373-386. https://doi.org/10.1590/1678-4685-gmb-2016-0087
Ebru U, Burak U, Yusuf S, Reyhan B, Arif K, Faruk TH, Emin M, Aydın K, Lhan Atilla I, Semsettin S, Kemal E. Cardioprotective effects of Nigella sativa oil on cyclosporine A‐induced cardiotoxicity in rats. Basic and Clinical Pharmacology and Toxicology 2008; 103(6):574-580. https://doi.org/10.1111/j.1742-7843.2008.00313.x
Massadeh AM, Al-Safi SA, Momani IF, Al-Mahmoud M, Alkofahi AS. Analysis of cadmium and lead in mice organs. Biological Trace Element Research 2007; 115(2):157-167. https://doi.org/10.1007/BF02686027
Adam GO, Rahman MM, Lee SJ, Kim GB, Kang HS, Kim JS, Kim SJ. Hepatoprotective effects of Nigella sativa seed extract against acetaminophen-induced oxidative stress. Asian Pacific Journal of Tropical Biomedicine 2016; 9(3):221-227. https://doi.org/10.1016/j.apjtm.2016.01.039
Abdel-Daim MM, Ghazy EW. Effects of Nigella sativa oil and ascorbic acid against oxytetracycline-induced hepato-renal toxicity in rabbits. Iranian Journal of Basic Medical Sciences 2015; 18(3): 221-227.
Hannan A, Saleem S, Chaudhary S, Barka M, Arshad MU. Anti-bacterial activity of Nigella sativa against clinicalisolates of methicillin resistant Staphylococcus aureus. Journal of Ayub Medical College, Abbottabad 2008; 20(3):72-74.
Morsi NM. Antimicrobial effect of crude extracts of Nigella sativa on multiple antibiotics-resistant bacteria. Acta Microbiologica Polonica 2000; 49(1):63-74
Nemmar A1, Al-Salam S, Zia S, Marzouqi F, Al-Dhaheri A, Subramaniyan D, Dhanasekaran S, Yasin J, Ali BH, Kazzam EE. Contrasting actions of diesel exhaust particles on the pulmonary and cardiovascular systems and the effects of thymoquinone. British Journal of Pharmacology 2011; 164(7):1871-1882. https://doi.org/10.1111/j.1476-5381.2011.01442.x
Alkharfy KM, Ahmad A, Khan RM, Al-Asmari M. High-performance liquid chromatography of thymoquinone in rabbit plasma and its application to pharmacokinetics. Journal of Liquid Chromatography & Related Technologies 2013; 36(16):2242-2250. https://doi.org/10.1080/10826076.2012.717062
Liaudanskas M, Viškelis P, Raudonis R, Kviklys D, Uselis N, Janulis, V. Phenolic composition and antioxidant activity of malus domestica leaves. The Scientific World Journal 2014, 2014: ID 306217, 10 pages. https://doi.org/10.1155/2014/306217
Published
Abstract Display: 707 
PDF Downloads: 592 PDF Downloads: 1069 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.