Molecular Characterization of Carbapenemase-Producing Enterobacterales in Children with Diarrhea in Rural Burkina Faso

  • René Dembélé Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso. https://orcid.org/0000-0002-9282-8555
  • Issiaka Soulama National Centre for Research and Training on Malaria, 01 BP 2208 Ouagadougou 01, Burkina Faso.
  • Wendpoulomdé Aimé Désiré Kaboré Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
  • Ali Konaté Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Assèta Kagambèga Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • David Coulibaly N'Golo Molecular Biology Platform, Pasteur Institute of Abidjan, Ivory Coast, 01 BP 490 Abidjan 01.
  • Oumar Traoré Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
  • Abdoulaye Seck Laboratory of Biological and Medical Analyzes, Pasteur Institute of Dakar, Dakar, Senegal.
  • Alfred Sababenedyo Traoré Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Nathalie Guessennd Laboratory of Bacteriology-Virology, Unit of Antibiotics, Natural Substances and Surveillance of Resistance of Microorganisms to Antimicrobials/ Pasteur Institute of Abidjan, Ivory Coast, 01 BP 490 Abidjan 01.
  • Amy Gassama-Sow Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 avenue Pasteur, BP 220, Dakar, Senegal.
  • Nicolas Barro Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

Abstract

Background and objective: In recent years, carbapenemase-producing Enterobacterales (CPE) resistance to antibiotics has dramatically increased leading to limitations of their treatment options. In the present study, we investigated the occurrence of carbapenemase-producing Escherichia coli and Salmonella in rural Burkina Faso.
Materials and methods: Salmonella isolates were serotyped according to the Kauffman White scheme. Diarrheagenic Escherichia coli (DEC) strains was identified using 16-plex Polymerase Chain Reaction (PCR), whereas antibiotic susceptibility was realized using the disk diffusion method. Furthermore, multiplex PCR assays were carried out using oligonucleotides to detect the presence of genes of the blaKPC, blaVIM, blaIMP, blaTEM, blaSHV, blaOXA and blaCTX-M types in all E. coli and Salmonella strains.
Results: The study highlighted high resistance rates of the identified bacteria to common antibiotics. Likewise, two strains of E. coli were imipenem resistant with carbapenemase-encoding genes. The genes detected were Klebsiella pneumoniae carbapenemase (KPC), Verona integrin-encoded metallo-β-lactamase (VIM) and Imipenemase (IMP-2) reaching a rate of 40% each in E. coli strains. However, no Salmonella carbapenemases blaKPC, blaVIM or blaIMP were detected.
Conclusion: This study showed that for a real-time infection control and prompt application of antimicrobial chemotherapy, characterization of carbapenemase-producing Enterobacterales in patients is crucial.
Keywords: Antibiotics, Carbapenemase-Producing Enterobacterales, children, Burkina Faso

Keywords: Antibiotics, Carbapenemase-Producing Enterobacterales, children, Burkina Faso

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Author Biographies

René Dembélé, Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.

Training and Research Unit in Applied Sciences and Technologies/University of Dedougou, BP 176 Dedougou, Burkina Faso.

Issiaka Soulama, National Centre for Research and Training on Malaria, 01 BP 2208 Ouagadougou 01, Burkina Faso.

National Centre for Research and Training on Malaria, 01 BP 2208 Ouagadougou 01, Burkina Faso.

Wendpoulomdé Aimé Désiré Kaboré, Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

Ali Konaté, Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.

Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.

Assèta Kagambèga, Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.

Institute of Sciences, 01 BP 1757 Ouagadougou 01, Burkina Faso.

David Coulibaly N'Golo, Molecular Biology Platform, Pasteur Institute of Abidjan, Ivory Coast, 01 BP 490 Abidjan 01.

Molecular Biology Platform, Pasteur Institute of Abidjan, Ivory Coast, 01 BP 490 Abidjan 01.

Oumar Traoré, Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

Training and Research Unit in Applied Sciences and Technologies/University of Dedougou, BP 176 Dedougou, Burkina Faso.

Abdoulaye Seck, Laboratory of Biological and Medical Analyzes, Pasteur Institute of Dakar, Dakar, Senegal.

Faculty of Medicine, Pharmacy and Odontology, University of Cheikh Anta DIOP of Dakar,

Alfred Sababenedyo Traoré, Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.

Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.

Nathalie Guessennd, Laboratory of Bacteriology-Virology, Unit of Antibiotics, Natural Substances and Surveillance of Resistance of Microorganisms to Antimicrobials/ Pasteur Institute of Abidjan, Ivory Coast, 01 BP 490 Abidjan 01.

Laboratory of Bacteriology-Virology/Unit of Training and Research of Medical Sciences/University Felix Houphouet BOIGNY, 01 BP V34 Abidjan 01, Ivory Coast.

Amy Gassama-Sow, Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 avenue Pasteur, BP 220, Dakar, Senegal.

Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 avenue Pasteur, BP 220, Dakar, Senegal.

Nicolas Barro, Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food/Center for Research in Biological, Food and Nutritional Sciences/Graduate School of Science and Technology/University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

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Dembélé R, Soulama I, Kaboré WAD, Konaté A, Kagambèga A, N’Golo DC, Traoré O, Seck A, Traoré AS, Guessennd N, Gassama-Sow A, Barro N. Molecular Characterization of Carbapenemase-Producing Enterobacterales in Children with Diarrhea in Rural Burkina Faso. JDDT [Internet]. 18Jan.2021 [cited 22Sep.2021];11(1):84-2. Available from: https://jddtonline.info/index.php/jddt/article/view/4513