Study on the biological safety of TiO2 nanoparticles based on the oxidative stress pathway
Objective: To study the effects of TiO2 nanoparticles in mice and explore the biological safety of TiO2 nanoparticles. Methods: The biological safety of TiO2 nanoparticles based on the oxidative stress pathway is investigated. One hundred mice were randomly divided into 10 groups. A low medium and high concentration experimental group and negative and positive control groups were investigated for both the intravenous injection and tracheal injection exposure experiments. Mice were observed for any symptoms, and the serum and lung homogenate for activity of SOD and content of MDA in serum and lung tissue were prepared. The scores of the allergic reaction determined the degree of anti-oxidation and the comprehensive experimental data determined the degree of injury in the mice. Results: An increase of TiO2 concentration in the nanomaterials led to a decrease of the content of MDA in serum and lung tissue of mice and the activity of SOD increased, indicating that mice showed oxidative stress in vivo. Conclusion: The strong direct exposure to a high concentration of TiO2 nanoparticles was harmful to the organism.1
Key words: Foundation medicine; TiO2; Nanoparticles; Oxidative stress; Biosafety nanomaterials
2. Chen X, Mao SS. Titanium dioxide nanomaterials: synthesis, properties, modifications, and applications. Chem Rev, 2007; 107:2891-2959.
3. Song Z, Hrbek J, Osgood R. Formation of TiO2 nanoparticles by reactive-layer-assisted deposition and characterization by XPS and STM. NanoLett, 2005; 5:1327-1332.
4. Kocbek P, Teskac K, Kreft ME, et al. Toxicological aspects of long-term treatment of keratinocytes with ZnO and TiO2 nanoparticles. Small, 2010; 6:1908-1917.
5. Chen X, Mao SS. Titanium dioxide nanomaterials: synthesis, properties, modifications, and applications. Chem Rev, 2007; 107:2891-2959.
6. Ying Jia Li, Zhang Ting, Tang Meng, Quantitative study on the relationship between physical and chemical properties and its application in the study of metal nano-materials toxicity, Chinese Journal of Pharmacology and Toxicology, 2014; 28(6):947~591
7. Zhu Shanshan, Zn0.4Fe2.6O4 Toxicity Evaluation of Nanoparticles in Mice [D]Beijing: Department of Inorganic Chemistry. 2014
8. Yang L, Kuang H J,Zhang W Y, et al. Size dependent biodistribution and toxicokinctics of iron oxide magnetic nanoparticles in mice, Nanoscale, 2015;7(2):625~636.
9. Bhattacharya D,Santra C R, Ghosh A N, et al.Differential Toxicity of Rod and Spherical Zine Oxide Naoparticles on Hunman Peripheral Blood Monomuclear Cells, Journal of Biomedical Nanotechnology, 201410(4):707-16
10. WANG Meng, GONG Shuang-Jiao, LIU Shan-Shan et al. Effects of surfactants on the ecotoxicological effects of single and combined pollution of nano-sized TiO2 and Cd in sediments. JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY, 2015; (5):791~797
11. Xia T, Kovochich M, Brant J et al. Comparision of the abilities of ambient and manufactured nanoparticles to induce celluar toxicity according to an oxidative stress paradigm. Nanoletters, 2006;
12. Pantarotto D, Briand JP, Prato M et al. Translocation of bioactive peptides across cell membranes by carbon nanotubes. Chem Commun Camb, 2004; 7:16-17.
13. Niu Hong-hong, Dong Huai-tai, Yang Li-li, et al. Effects of Nanometer Titanium Dioxide with Different Particle Size on Antioxidant Capacity in Mice. Journal of Hebei Medical Universityï¼Œ2015; (36):184-187
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).