HYPOGLYCEMIC POTENTIAL OF PROBIOTIC DNA LOADED CHITOSAN NANOPARTICLES: AN IN VIVO STUDY
Abstract
The rise in infectious diseases as well as noninfectious immune related disorders causes millions of premature deaths worldwide and demands the need for the development of efficient immunomodulators. Consumer awareness about the harmful effects of chemical drugs raised a need to search for natural/ alternative therapies for the treatment of diseases. Immunotherapy is one of the alternative ways of modification of diseases. Some bacterial cell components such as peptidoglycans, lipoteichoic acid, secreted soluble substances, genomic DNA, etc, play role in immunomodulation responses. Structural difference between bacterial and eukaryotic DNA apparently account for the ability of bacterial DNA to serve as an immune activating agent, because of its high content of unmethylated CpG dinucleotides. Probiotic DNA’s efficacy could be further enhanced by loading it onto nanoparticles. Nanoparticle-assisted delivery may be a promising approach to alleviate the problem of instability and degradation of DNA. The present study was conducted to prepare and characterize the probiotic DNA loaded chitosan nanoparticles (DLCNP) and to find out the anti-diabetic potential of probiotic bacterial DNA Lactobacillus acidophilus NCDC 343 (LA DNA). Therapeutic efficacy of Probiotic DNA loaded with Chitosan Nanoparticles was evaluated in treating diabetic Balb/c mice. DNA loaded Chitosan Nanoparticles proved as effective anti-diabetic agents and in addition, the bioactivity of probiotic DNA was improved through nano delivery.
Keywords: Probiotics, Probiotic DNA, Chitosan-DNA Nanoparticles, Immunotherapy, Anti-diabetic/ Hypoglycemic activity
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Keywords:
Probiotics, Probiotic DNA, Chitosan DNA Nanoparticles, Immunotherapy, Anti-diabetic/Hypoglycemic activityDOI
https://doi.org/10.22270/jddt.v7i2.1405References
Kaveeshwar SA, Cornwall J. The current state of diabetes mellitus in India. Australas Med J. 2014; 7(1):45–48.
Ryan G, Briscoe TA, Lynette L. Review of pramlintide as adjunctive therapy in treatment of type 1 and type 2 diabetes. Drug Des Devel Ther. 2009; 2:203–214.
McFarland LV. Meta-analysis of probiotics for the prevention of antibiotic associated diarrhoea and the treatment of Clostridium difficile disease. Am J Gastroentrol. 2006; 101(4):812-822.
McCabe LR, Irwin R, Schaefer L, Britton RA. Probiotic use decreases intestinal inflammation and increases bone density in healthy male but not female mice. J Cell Physiol. 2013; 228(8):1793–1798.
Krammer HJ, Kamper H, Von Banau R, Zieseniss E, Stange C, Schlieger F, Clever I, Schulae J. Probiotic drug therapy with E.coli strain Nissle 1917 (EcN): results of a prospective study of the secords of 3807 patients. Z Gastroentrol. 2005; 44:651-656.
Randhawa MK, Bhatia A, Ali SA, Balgir PP. In vivo comparison of probiotic bacteria’s with their DNA: As augmenter of immune efficacy, Adv Appl Sci Res. 2012; 3:826-832.
Ghadimi D, Holst RF, de Vrese M, Winkler P, Heller KJ, Schrezenmeir J. Effects of Probiotic bacteria and their genomic DNA on TH1/TH2- cytokine production by peripheral blood mononuclear cells (PMBCs) of healthy and allergic subjects. Immunobiology. 2008; 213:677-692.
Krieg AM. Cp G motifs in Bacterial DNA and their immune effects. Annu Rev Immunol. 2002; 20:709-760.
Tiyaboonchai W. Chitosan Nanoparticles: A Promising System for Drug Delivery. Naresuan Univ J. 2003; 11(3):51-66.
Rajalakshmi R, Indira MY, Aruna U, Vinesha V, Rupangada V, Krishna moorthy SB. Chitosan Nanoparticles - An Emerging Trend In Nanotechnology. Int J Drug Delivery, 2014; 6:204-229.
Bhatia A, Shard P, Chopra D, Mishra T. Chitosan nanoparticles as Carrier of Immunorestoratory plant extract: synthesis, characterization and Immunorestoratory efficacy. Int J Drug Delivery. 2011; 3:381-385.
Atashpaz S, Khani S, Barzegari A, Barar J, Vahed SZ, Azarbaijani R, Omidi YA Robust Universal Method for Extraction of Genomic DNA from Bacterial Species. Microbiology. 2010; 79 (4):538–542.
Chew JL, Wolfowicz CB, Mao HQ, Leong KW, Chuaa KY .Chitosan nanoparticles containing plasmid DNA encoding house dust mite allergen, Der p 1 for oral vaccination in mice. Vaccine. 2003; 21:2720–2729.
Silva VJDD. Preparation and characterization of chitosan nanoparticles for gene delivery. Technico Lisboa. 2013; 1-10.
Akbuga J, Turan SO, Erdogan N. Plasmid-DNA loaded chitosan microspheres for in vitro IL-2 expression. European J Pharm Biopharmaceutics. 2004; 58:501–507.
Ozbek H, Ceylan E, Kara M, Ozgokce F, Koyuncu M. Hypoglycemic effect of Rheumribes roots in alloxan induced diabetic and normal mice. Scand J Lab Anim Sci. 2004; 31:113-115.
Gao X, Huang L. Potentiation of cationic liposome-mediated gene delivery by polycations. Biochemistry. 1996; 35:1027–1036.
Hudson L, Hay FC. A handbook of practical immunology (3rd ed.), Oxford: Blackwell scientific publication, London. 1989.
Mathew G, Glenda J, Jack L .Quantitaion of nitrite and nitrate ions in extracellular fluids. Enzymology. 1996; 268:237-246.
Matsuzaki T, Chin J. Modulating immune responses with probiotic bacteria. Immunol. Cell Biol. 2000; 78(1):67-73.
Bhatia A, Pawan R. Therapeutic effect of probiotic immune response and hypercholesteremia: an experimental study. Res J Biotech. 2007; 2 (3):43-46.
Kwon K. Generation of regulatory dendritic cells and CD4+ Foxp3+ T cells by probiotics administration suppresses immune disorders. PNAS. 2010; 107(5): 2159-2164.
Bhatia A, Kaur M, Kaur H. Comparison of Immunomodulatory Activity of Probiotic Bacteria and their DNA: A Study Conducted with L. acidophilus NCDC343 & L. casei Isolated from Yakult. Int J Pharm Res Scholars. 2015; 4(1)
Hellum, K.B. Nitroblue Tetrazolium test in bacterial and viral infections. Scand J Infect Dis. 1977; 9:269-276.
Szczylik C, Gornas P, Carewicz R. NBT reduction test methods and practical use. Diagn Lab. 1979; 1:35-40.
Klinman DM, Yi A, Beaucage SL, Conover J, Krieg AM. CpG motifs expressed by bacterial DNA rapidly induce lymphocytes to secrete IL-6, IL-12 and IFNg. Proc Natl Acad Sci. 1996; 93:2879–2883.
Chuang L, Wu KG, Pai C, Hsieh PS, Tsai JJ, Yen JH, Lin MY. Heat-killed cells of lactobacilli skew the immune response towards T helper 1 polarization in mouse splenocytes and dendritic cell-treated T cells. J Agr Food Chem. 2007; 55(26):11080-11086.
Tabuchi M, Ozaki M, Tamura A, Yamada N, Ishida T, Hosoda M, Hosono A. Antidiabetic effect of Lactobacillus GG in streptozotocin induced diabetic rats. Biosci Biotechnol Biochem. 2003; 67(6):1421-4.
Yadav, H., Jain, S. and Sinha, P.R.2007.Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei in high fructose fed rats. Nutr. 23(1):62-8.
Bhatia A, Mishra T. Hypoglycemic activity of Ziziphus mauritiana aqueous ethanol seed extract in alloxan-induced diabetic mice. Pharma Biol. 2010; 48 (6):604-610.
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