ISOLATION AND CHARACTERIZATION OF WITHAFERIN-A FROM THE WITHANIA SOMNIFERA (ASHWAGANDHA)

  • Bali Reddy Keesara Institute of Pharmacy, Shri Jagdishprasad Jhabarmal Tibrewala University Jhunjhunu Rajasthan, 313001, India
  • Rakesh Kumar Jat Institute of Pharmacy, Shri Jagdishprasad Jhabarmal Tibrewala University Jhunjhunu Rajasthan, 313001, India

Abstract

The research work is related to an improved process of isolation & characterization of Withaferin-A from withania somnifera (Sanskrit: Ashawagandha, English: Winter cherry). Withania somnifera, commonly known as Ashwagandha, is a valued herb in Ayurvedic medicine. Roots, leaves and preparations of the plant are traditionally used as tonic, hypnotic, sedative and diuretic. W. somnifera mainly contains withanolides including withaferin-A which are specific to the Solanaceae family. Withanolides are biologically active secondary metabolites present in roots and leaves of W.somnifera. In the present study, we have standardized the protocol for the isolation of Withaferin-A from the Ashwagandha punchang. Withaferin A possess anti-inflammatory and anti-stress properties. This study contains newer and conventional method of isolation of Withaferin-A from Withania somnifera as well Quantitative and qualitative techniques involved in the purification of the compound was followed throughout this research work. In this study, we have taken different trials based on hydro-alcoholic solvent composition. Based on different solvent extraction process pure 98% Withaferin-A was successfully isolated.

Keywords: Withania somnifera, Withaferin-A, Isolation, TLC, HPLC.

 

 

Downloads

Download data is not yet available.

Author Biographies

Bali Reddy Keesara, Institute of Pharmacy, Shri Jagdishprasad Jhabarmal Tibrewala University Jhunjhunu Rajasthan, 313001, India

Institute of Pharmacy, Shri Jagdishprasad Jhabarmal Tibrewala University Jhunjhunu Rajasthan, 313001, India

Rakesh Kumar Jat, Institute of Pharmacy, Shri Jagdishprasad Jhabarmal Tibrewala University Jhunjhunu Rajasthan, 313001, India
Institute of Pharmacy, Shri Jagdishprasad Jhabarmal Tibrewala University Jhunjhunu Rajasthan, 313001, India

References

1. "The Value of Plants Used in Traditional Medicine for Drug Discovery". Environmental Health Perspectives, Reviews in Environmental Health. 2001, 109(1).
2. Nayak S, Suryawanshi S, Vaidhun B, Microneedle technology for transdermal drug delivery: applications and combination with other enhancing techniques, Journal of Drug Delivery & Therapeutics. 2016; 6(5):84-90. DOI: http://dx.doi.org/10.22270/jddt.v6i5.1276
3. Mukherjee PK, GMP for Indian Systems of Medicine, Business Horizons, New Delhi, 2003.
4. Boparai A, Niazi J, Bajwa N, Singh AP, A Review Update on Dillenia Indica F. Elongata (MIQ.)MIQ., Journal of Drug Delivery & Therapeutics. 2016; 6(2):62-70. DOI: http://dx.doi.org/10.22270/jddt.v6i5.1226
5. Andrew Vickers and Catherine Zollman, "ABC of complementary medicine: Herbal medicine - Clinical review". Br Med J. 1999, 16.
6. Kalaiselvi Senthil, et al, “A simple method to purify withanolide A from the roots of Withania somnifera dunal” International Journal of Pharma and Bio Sciences; 2011; 2(2)
7. Geeta singh et al, “Antimicrobial efficacy of alkaloids of Withania somnifera (Ashwagandha) an indigenous medicinal plant against some pathogens”, Journal of Pharmacy Research; 2011; 4(3):807-809.
8. Shoma Paul Nandi, et al, “Antioxidant and antimicrobial activities of aqueous extract of Withania somnifera against methicillin-resistant Staphylococcus aureus”, Journal of Microbiology and Biotechnology Research, J. Microbiol. Biotech. Res; 2011, 1 (1): 40-45.
9. Zaved Ahmed Khan, et al, “L-Arginine abolishes the anxiolytic-like effect of withaferin A in the elevated plus-maze test in rats”, African Journal of Pharmacy and Pharmacology. 2011; 5(2):234-237.
10. B. Bhosale et al, “HPLC Methods of medicinally important plants: a review”, Imperial J. Pharmacognosy & Natural Products. 2011; 1(1).
11. A. Singh, et al, “Effect of time on extraction of ashwagandha in various hydro alcoholic compositions and their anti-inflammatory activity”, international journal of green pharmacy; January-march 2011; 69-74.
12. R. Kushwaha, et al, “Standardization of Ashwagandharishta formulation by TLC Method” , International Journal of ChemTech Research, July-Sept 2011; 3(3):1033-1036,
13. S. Verma, et al, “Therapeutic uses of withania somnifera (ashwagandha) with a note on withanolides and its pharmacological actions”, Asian Journal of Pharmaceutical and Clinical Research; 2011; 4(Supply 1).
14. V. Sharma, et al, “Withania somnifera: A Rejuvenating Ayurvedic Medicinal Herb for the Treatment of various Human ailments”, International Journal of PharmTech Research; 2011; 3(1):187-192.
15. Z.A. Khan, et al, “Possible binding modes of withaferin-A and its analogs in the active site of NNOS molecular docking studies”, International Journal of Pharma and Bio Sciences; 2011; 2(3).
Statistics
381 Views | 295 Downloads
How to Cite
Keesara, B., & Jat, R. (2017). ISOLATION AND CHARACTERIZATION OF WITHAFERIN-A FROM THE WITHANIA SOMNIFERA (ASHWAGANDHA). Journal of Drug Delivery and Therapeutics, 7(1), 65-69. https://doi.org/10.22270/jddt.v7i1.1375