Analytical Study of Terminalia catappa L. Seeds from Central India: A Pharmacognostic, HPTLC, and FTIR-Based Approach
Abstract
Background- Terminalia catappa L., widely recognized in traditional medicine, has demonstrated hepatoprotective, antioxidant, and antimicrobial effects. However, phytochemical variability due to geographical differences hampers its standardization. This study aimed to establish a pharmacognostic and phytochemical profile of T. catappa seeds from the Central Indian Deccan Plateau, integrating modern spectroscopic and chromatographic tools.
Method- Mature seeds were collected from Bhopal, Madhya Pradesh, and authenticated. Pharmacognostic evaluations included transverse and powder microscopy, while physicochemical parameters (total ash, extractive values, moisture content) were measured per WHO and Ayurvedic Pharmacopoeia guidelines. Phytochemical constituents were identified via standardized tests on methanolic and aqueous extracts. Soxhlet extraction estimated total fat content. Fourier-transform infrared (FTIR) spectroscopy and high-performance thin-layer chromatography (HPTLC) were employed for molecular and marker compound analysis
Results-Microscopic analysis revealed characteristic features such as xylem tracheid, oil globules, stone cells, and calcium oxalate crystals. Physicochemical analysis yielded total ash (4.54%), alcohol-soluble extractive (16.39%), and moisture content (2.29%). Phytochemical screening confirmed the presence of alkaloids, flavonoids, proteins, and saponins, with notable absence of tannins. HPTLC detected β-sitosterol (255–268.7 µg/mL) with high reproducibility (CV = 3.69%). FTIR spectra revealed functional groups corresponding to phenolics, flavonoids, fatty acids, esters, and proteins.
Conclusion-This is the first standardized pharmacopeial profile of T. catappa seeds from Central India. Integration of classical pharmacognosy with FTIR and HPTLC establishes a replicable quality control framework for geographically distinct ecotypes. These findings support the nutraceutical and therapeutic potential of T. catappa seeds and lay the groundwork for future bioactivity validation and industrial application.
Keywords: FTIR spectroscopy, HPTLC, Pharmacognostic evaluation, Phytochemical profiling, Terminalia catappa.
Keywords:
FTIR spectroscopy, HPTLC, Pharmacognostic evaluation, Phytochemical profiling, Terminalia catappaDOI
https://doi.org/10.22270/jddt.v16i6.7773References
1. Pereira A, Sumathy A, Usha K. Terminalia catappa: Ethnopharmacology and therapeutic potential. J Ethnopharmacol. 2018;227:26-35.
2. Chaudhary G, Goyal S, Poonia P. Ayurvedic applications of Terminalia catappa:A review. J Ayurveda Integr Med. 2020;11(3):312-8.
3. Fan YM, Xu LZ, Gao J, Wang Y, Tang XH, Zhao XN, et al. Hepatoprotective activity of Terminalia catappa L. leaves and its two triterpenoids. Am J Chin Med.2004;32(4):509-19.
4. Ko TF, Weng YM, Lin SB, Chiou RY. Antioxidant capacity of Terminalia catappa leaves extracts and its major flavonoids. Food Chem. 2007;100(4):1539-45.
5. Khan AV, Ahmed QU, Mir MR, Shakil S, Khan AA. Geographic variation in phenolic composition of Terminalia catappa L. leaves. Nat Prod Res. 2015;29(7):621-7.
6. Patra JK, Rath SK, Jena KB, Rathod VK, Thatoi HN. Chemical composition and antioxidant potential of Terminalia catappa Linn. fruit extracts. J Food Biochem. 2011;35(5):1530-41.
7. Ministry of AYUSH. The Ayurvedic Pharmacopoeia of India. Part I, Vol. IX. New Delhi: Government of India; 2016.
8. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman & Hall; 1998.
9. Kumar V, Kumar M, Agrawal S, Singh S. Soil-climate-phytochemistry relationships of medicinal plants in Central India. Ind Crops Prod. 2019;142:111857.
10. Schulz H, Baranska M. Identification and quantification of valuable plant substances by IR and Raman spectroscopy. Vib Spectrosc. 2007;43(1):13-25. https://doi.org/10.1016/j.vibspec.2006.06.001
11. World Health Organization. Quality control methods for herbal materials. Geneva: WHO Press; 2011.
12. Trease GE, Evans WC. Pharmacognosy. 16th ed. Edinburgh: Saunders Elsevier; 2009. p. 545-547.
13. Wallis TE. Textbook of Pharmacognosy. 5th ed. New Delhi: CBS Publishers & Distributors; 2005. p. 123-145.
14. Government of India, Ministry of AYUSH. The Ayurvedic Pharmacopoeia of India, Part I, Vol. I. 1st ed. New Delhi: Department of AYUSH; 2001. p. 143-145.
15. Kokate CK, Purohit AP, Gokhale SB. Practical Pharmacognosy. 4th ed. Pune: Nirali Prakashan; 2005. p. 107-111.
16. Sharma H, Narshimhaji CV, Singh R. Relative estimation of beta-sitosterol in alcoholic extracts of roots and small branches of oroxylum indicum (L.) Kurz by HPTLC for plant part substitution, medicinal uses and conservation. Journal of Applied Research on Medicinal and Aromatic Plants. 2023 May 1;35:100496. https://doi.org/10.1016/j.jarmap.2023.100496
17. Joshi DD. FTIR spectroscopy: Herbal drugs and fingerprints. In Herbal Drugs and Fingerprints: Evidence Based Herbal Drugs 2012 Oct 3 (pp. 121-146). India: Springer India. https://doi.org/10.1007/978-81-322-0804-4_7
18. Khan AV, Ahmed QU, Mir MR, Shakil S, Khan AA. Geographic variation in phenolic composition of Terminalia catappa L. leaves. Nat Prod Res. 2015;29(7):621-7.
19. Wallis TE. Textbook of Pharmacognosy. 5th ed. New Delhi: CBS Publishers & Distributors; 2005. p. 123-145.
20. Akinsulire OP, Oladipo OT, Illoh HC, Mudasiru OM. Vegetative and reproductive morphological study of some species in the family Combretaceae in Nigeria. Ife Journal of Science. 2018 Aug 13;20(2):371-89. https://doi.org/10.4314/ijs.v20i2.18
21. World Health Organization. Quality control methods for herbal materials. Geneva: WHO Press; 2011.
22. Mwangi WC, Waudo W, Shigwenya ME, Gichuki J. Phytochemical characterization, antimicrobial and antioxidant activities of Terminalia catappa methanol and aqueous extracts. BMC Complementary Medicine and Therapies. 2024 Apr 2;24(1):137. https://doi.org/10.1186/s12906-024-04449-7 PMid:38566161 PMCid:PMC10986026
23. Gopalam R, Manasa V, Vaishnav SR, Daga P, Tumaney AW. Profiling of lipids, nutraceuticals, and bioactive compounds extracted from an oilseed rich in PUFA. Plant Foods for Human Nutrition. 2022 Mar;77(1):98-104. https://doi.org/10.1007/s11130-021-00945-0 PMid:35088213
24. Zannou O, Awad NM, Ballogou VY, Mohammed S, Miassi YE, Houngbédji M, Dossa KF, Abdoulaye A, Ghellam M, Kpoclou YE, Aïssi MV. Tropical Almond Tree (Terminalia catappa L.): A Comprehensive Review of the Phytochemical Composition, Bioactivities and Economic Potential. Pharmaceuticals. 2026 Jan 5;19(1):99. https://doi.org/10.3390/ph19010099 PMid:41599698 PMCid:PMC12845074
25. Sene CF, McCann MC, Wilson RH, Grinter R. Fourier-transform Raman and Fourier-transform infrared spectroscopy (an investigation of five higher plant cell walls and their components). Plant physiology. 1994 Dec 1;106(4):1623-31. https://doi.org/10.1104/pp.106.4.1623 PMid:12232436 PMCid:PMC159706
26. Sahoo MR, Umashankara MS. FTIR based metabolomics profiling and fingerprinting of some medicinal plants: an attempt to develop an approach for quality control and standardization of herbal materials. Pharmacogn. Res. 2022;15:163-7. https://doi.org/10.5530/097484900288
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