Preliminary phytochemicals and evaluation of the hypolipidemic effect of a saponin from Asparagus officinalis L. roots in hyperlipidemic rats
Abstract
Hyperlipidemia, a condition of elevated lipids in the blood, is a major risk factor for atherosclerosis and subsequently cardiovascular disease (CVD), a leading cause of mortality worldwide. This study aimed to evaluate the hypolipidemic properties of the saponin extracted from the roots of A. officinalis (AOe) against Swiss albino Wistar rats. The root extract of A. officinalis was screened for its phytochemical investigation. Initial phytochemical analysis confirmed the presence of saponins. The root extract of A. officinalis underwent Soxhlet extraction with a solvent, followed by fractionation with n-butanol to isolate the saoponin-rich fraction. The separated fraction was orally administered to Triton-induced hyperlipidemic Wistar rats for 28 days at increasing doses of 100, 200, and 300 mg/kg body weight. At the end of treatment, serum lipid profiles, including total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL), were assessed and compared with those of a conventional hypolipidemic agent (atorvastatin). The present study found that the saponin-rich fraction therapy from A. officinalis significantly (p<0.001) attenuated the elevation of total cholesterol (TC), triglycerides, low-density lipoprotein (LDL) levels, and very low-density lipoprotein (VLDL), coupled with a concurrent improvement in high-density lipoprotein (HDL) cholesterol, demonstrating pronounced hypolipidemic efficacy. This study demonstrates the hypolipidemic efficacy of the saponin fraction from A. officinalis in rats, exhibiting a reduction in bad cholesterol and an increase in good cholesterol. These findings suggest it could serve as a viable alternative for managing hyperlipidemia.
Keywords: Hyperlipidemia, A. officinalis, Triton, Soxhlet, total cholesterol, hypolipidemic
Keywords:
Hyperlipidemia, A. officinalis, Triton, Soxhlet, total cholesterol, hypolipidemicDOI
https://doi.org/10.22270/jddt.v15i10.7385References
1. Oliveira Lopes RH, Benitez Macorini LF, Antunes KÁ, Espindola T, Alfredo TM, Pereira ZV, et al. Antioxidant and hypolipidemic activity of the hydroethanolic extract of Curatella americana L. leaves. Oxidative Medicine and Cellular Longevity 2016;2016:9681425. https://doi.org/10.1155/2016/9681425 PMid:27247703 PMCid:PMC4876233
2. Hypolipidemic, antioxidant and anti-atherosclerogenic effect of aqueous extract leaves of Cassia occidentalis Linn (Caesalpiniaceae) in diet-induced hypercholesterolemic rats. BMC Complementary and Alternative Medicine 2017;17:76. https://doi.org/10.1186/s12906-017-1566-x PMid:28122565 PMCid:PMC5264340
3. World Health Organization. Global report on diabetes. Geneva: World Health Organization; 2023.
4. Al Hawat L, Alallan L. Estimation of antioxidant and hypolipidemic activities of extracts of Citrus x aurantium leaves in vitro. Phytomedicine Plus 2025;5(1):100723. https://doi.org/10.1016/j.phyplu.2024.100723
5. Abbasi S, Khan A, Choudhry MW. New insights into the treatment of hyperlipidemia: pharmacological updates and emerging treatments. Cureus 2024;16(6):e63078. https://doi.org/10.7759/cureus.63078
6. Kanwal Q, Ahmed M, Ur-Rehman A, Anwar A, Shahid S, Shahzad A, et al. Hypolipidemic effect of chloroform extract of Lagenariasiceraria: potential inhibitory activity of phytochemicals targeting the HMG-CoA reductase revealed by molecular docking and simulation studies. Journal of Chemistry 2023;2023:3010463. https://doi.org/10.1155/2023/3010463
7. Gaggini M, Gorini F, Vassalle C. Lipids in atherosclerosis: pathophysiology and the role of calculated lipid indices in assessing cardiovascular risk in patients with hyperlipidemia. International Journal of Molecular Sciences 2022;24(1):75. https://doi.org/10.3390/ijms24010075 PMid:36613514 PMCid:PMC9820080
8. Bays HE, Kirkpatrick C, Maki KC, Toth PP, Morgan RT, Tondt J, et al. Obesity, dyslipidemia, and cardiovascular disease: a joint expert review from the Obesity Medicine Association and the National Lipid Association 2024. Obesity Pillars 2024;10:100108. https://doi.org/10.1016/j.obpill.2024.100108 PMid:38706496 PMCid:PMC11066689
9. Gong X, Li X, Xia Y, Xu J, Li Q, Zhang C, et al. Effects of phytochemicals from plant-based functional foods on hyperlipidemia and their underpinning mechanisms. Trends in Food Science & Technology 2020;103:304-20. https://doi.org/10.1016/j.tifs.2020.07.026
10. Pan J, Ouyang X, Jin Q, Li P, Zhou L, He J, et al. Hypolipidemic effect of ethanol extract from Chimonanthus nitens Oliv. Leaves in hyperlipidemia rats via activation of the leptin/JAK2/STAT3 pathway. Molecular Medicine 2022;28:159. https://doi.org/10.1186/s10020-022-00589-z PMid:36539694 PMCid:PMC9768954
11. Xie W, Zhao Y, Du L. Emerging approaches of traditional Chinese medicine formulas for the treatment of hyperlipidemia. Journal of Ethnopharmacology 2012;140(2):345-67. https://doi.org/10.1016/j.jep.2012.01.027 PMid:22306102
12. Pegiou E, Mumm R, Acharya P, de Vos RCH, Hall RD. Green and white asparagus (Asparagus officinalis): a source of developmental, chemical and urinary intrigue. Metabolites 2019;10(1):17. https://doi.org/10.3390/metabo10010017 PMid:31881716 PMCid:PMC7022954
13. M A, A M, Ezzaitouni M, J L. Cytotoxicity and chemotaxonomic significance of saponins from wild and cultured asparagus shoots. Molecules 2023;29(14):3367. https://doi.org/10.3390/molecules29143367 PMid:39064945 PMCid:PMC11279782
14. Guo Q, Wang N, Liu H, Li Z, Lu L, Wang C. The bioactive compounds and biological functions of Asparagus officinalis L. - A review. Journal of Functional Foods 2020;65:103727. https://doi.org/10.1016/j.jff.2019.103727
15. Fuentes Alventosa JM, Moreno Rojas JM. Chapter 13 - Bioactive compounds in asparagus and impact of storage and processing. In: Preedy V, editor. Processing and Impact on Active Components in Food. San Diego: Academic Press; 2015. p. 103-10. https://doi.org/10.1016/B978-0-12-404699-3.00013-5
16. Zhu X, Zhang W, Pang X, Wang J, Zhao J, Qu W. Hypolipidemic effect of n-butanol extract from Asparagus officinalis L. in mice fed a high-fat diet. Phytotherapy Research 2011;25(8):1119-24. https://doi.org/10.1002/ptr.3380 PMid:21280112
17. Auwal MS, Saka S, Mairiga IA, Sanda KA, Shuaibu A, Ibrahim A. Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa). Veterinary Research Forum 2015;5(2):95-100.
18. Shaikh JR, Patil MK. Qualitative tests for preliminary phytochemical screening: an overview. International Journal of Chemical Studies 2020;8(2):603-8. https://doi.org/10.22271/chemi.2020.v8.i2i.8834
19. Edeoga HO, Okwu DE, Mbaeble BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 2005;4(7):685-8. https://doi.org/10.5897/AJB2005.000-3127
20. Abid R, Mahmood R, Santosh Kumar HS. Hypolipidemic and antioxidant effects of ethanol extract of Cassia fistula fruit in hyperlipidemic mice. Pharmaceutical Biology 2016;54(12):2822-9. https://doi.org/10.1080/13880209.2016.1185445 PMid:27256804
21. Cao S, Liu M, Han Y, Li S, Zhu X, Li D, et al. Effects of saponins on lipid metabolism: the gut-liver axis plays a key role. Nutrients 2023;16(10):1514. https://doi.org/10.3390/nu16101514 PMid:38794751 PMCid:PMC11124185
22. Livingston Raja NR, AathiraRavindran Nair, SwarnabalaSenthilpandian, Vijay Ravi. Hypolipidemic action of rutin on Triton WR-1339-induced hyperlipidemia in rats. Journal of Pre-Clinical and Clinical Research 2021;15(2):51-5. https://doi.org/10.26444/jpccr/136231
23. Nurcahyo H, Riyanta AB, Febriyanti R, Sutanto H, Herdwiani W. Hypolipidemic activity of Ceciwis ethanol extract on Wistar rats induced by high fat in vivo. Journal of Advanced Pharmacy Education & Research 2023;13(1):100-4. https://doi.org/10.51847/enXiIQzXM1
24. Mo H, Jeter R, Bachmann A, Yount ST, Shen CL, Yeganehjoo H. The potential of isoprenoids in adjuvant cancer therapy to reduce the adverse effects of statins. Frontiers in Pharmacology 2019;9:1515. https://doi.org/10.3389/fphar.2018.01515 PMid:30662405 PMCid:PMC6328495
25. Fidèle N, Joseph B, Emmanuel T, et al. Hypolipidemic, antioxidant and anti-atherosclerogenic effect of aqueous extract leaves of Cassia occidentalis Linn (Caesalpiniaceae) in diet-induced hypercholesterolemic rats. BMC Complementary and Alternative Medicine 2017;17:76. https://doi.org/10.1186/s12906-017-1566-x PMid:28122565 PMCid:PMC5264340
26. Xiao MY, Li S, Pei WJ, Gu YL, Piao XL. Natural saponins on cholesterol-related diseases: treatment and mechanism. Phytotherapy Research 2025;39(3):1292-318. https://doi.org/10.1002/ptr.8432 PMid:39754504
27. Kothari S, Jain AK, Mehta SC, Tonpay SD. Hypolipidemic effect of fresh Triticum aestivum (wheat) grass juice in hypercholesterolemic rats. ActaPoloniaePharmaceutica 2011;68(2):291-4.
28. García MD, Sáenz MT, Marquez-Martín A, Fernández-Arche MA. Hypocholesterolemic and Hepatoprotective Effects of "Triguero" Asparagus from Andalusia in Rats Fed a High Cholesterol Diet. Evidence-Based Complementary and Alternative Medicine:ECAM, 2011:2012:814752. https://doi.org/10.1155/2012/814752 PMid:22203881 PMCid:PMC3235947
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