Qualitative and Quantitative Analysis of Primary and Secondary Metabolites from Leptadenia pyrotechnica and Crataeva nurvala
Abstract
Plants are an abundant source of bioactive compounds that are valuable for medicinal and therapeutic applications. The present study investigates the qualitative presence of primary and secondary metabolites in Leptadenia pyrotechnica and Crataeva nurvala, two plants widely used in traditional medicine. Phytochemical screening revealed the presence of primary metabolites, including carbohydrates, proteins, and lipids, as well as secondary metabolites, such as alkaloids, flavonoids, and phenolic compounds. Quantitative analysis highlighted variations in metabolite composition between plant parts. Notably, Leptadenia pyrotechnica aerial parts exhibited high protein and lipid content, while Crataeva nurvala leaf showed significant phenol concentration. Our research demonstrates that Leptadenia pyrotechnica and Crataeva nurvala possess a rich array of bioactive compounds, validating their traditional medicinal applications. The identified metabolites exhibit potential antioxidant, anti-inflammatory, and antimicrobial properties, necessitating further investigation to characterize and quantify these compounds. This study lays the groundwork for future pharmacological studies and underscores the significance of preserving and sustainably utilizing these plant species in natural medicine. The results have far-reaching implications for the development of innovative therapeutic agents and the promotion of complementary and alternative medicinal practices.
Keywords: Leptadenia pyrotechnica, Crataeva nurvala, alkaloids, flavonoids, phytochemicals
Keywords:
Leptadenia pyrotechnica, alkaloids, flavonoids, phytochemicalsDOI
https://doi.org/10.22270/jddt.v15i6.7183References
1. Harborne JB. Textbook of Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 5th ed. London: Chapman and Hall Ltd; 1998. p. 21-72.
2. Jain V, Jain DK. Pharmacognostic and pharmacological studies on Leptadenia pyrotechnica (Forssk.) Decne. J Pharm Res. 2011;4(7):2521-2525.
3. Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. Springer; 2007. https://doi.org/10.1007/978-0-387-70638-2 PMCid:PMC2705749
4. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. Nirali Prakashan; 2009.
5. Lowry OH, Rose HN, Broug J, Farr AL, Randall RJ. Protein measurement with the Folin-phenol reagent. J Biol Chem. 1951;193:265-275. https://doi.org/10.1016/S0021-9258(19)52451-6 PMid:14907713
6. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28(3):350-356. https://doi.org/10.1021/ac60111a017
7. Jayaram M. Laboratory Manual in Biochemistry. Wiley Eastern Limited; 1981.
8. Bray HG, Thorpe WV. Analysis of phenolic compounds of interest in metabolism. Methods Biochem Anal. 1954;1:27-52. https://doi.org/10.1002/9780470110171.ch2 PMid:13193524
9. Verma N, Jha KK, Chaudhary S, Singh O, Kumar A. Phytochemistry, pharmacology and traditional uses of Leptadenia pyrotechnica- An important medicinal plant. Indian J Pharm Biol Res. 2014;2(1):128-134. https://doi.org/10.30750/ijpbr.2.1.20
10. Patel SK, Desai PR, Pandey VB. Ethnomedicinal plants used by the tribals in Bhiloda taluka of Sabarkantha district, Gujarat. Indian J Adv Plant Res. 2014;1:33-36
11. Kumari, D., Singh, D., Meena, M., Janmeda, P., & Siddiqui, M. H. Qualitative, Quantitative, In Vitro Antioxidant Activity and Chemical Profiling of Leptadenia pyrotechnica (Forssk.) Decne Using Advanced Analytical Techniques. Antioxidants, 2024;13(7):794. https://doi.org/10.3390/antiox13070794 PMid:39061862 PMCid:PMC11273909
12. El-Fitiany, R. A., & Khasawneh, M. A. Leptadenia Pyrotechnica (Forsk.) Decne: from edibility to drug discovery (a comprehensive review). Food Reviews International, 2023;39(9):6834-6886. https://doi.org/10.1080/87559129.2022.2126853
13. Aathira, K. K., Kariyil, B. J., Dhanusha, G., Haima, J. S., Sujith, S., Shynu, M., & Nisha, A. R. Qualitative and quantitative analysis (GC-MS) of methanol extract of Crataeva nurvala stem bark. 2021 https://doi.org/10.51966/jvas.2021.52.2.135-141
14. Kumar, D., Sharma, S., & Kumar, S. Botanical description, phytochemistry, traditional uses, and pharmacology of Crataeva nurvala Buch. Ham.: an updated review. Future journal of Pharmaceutical sciences, 2020;6:1-10. https://doi.org/10.1186/s43094-020-00106-1
15. Masood, N., Jamil, Q., Aslam, M. I., Masood, M. I., Shirazi, J. H., Jamil, Q. A., ... & Iqbal, S. M. Antioxidant, carbonic anhydrase inhibition and diuretic activity of Leptadenia pyrotechnica Forssk. Decne. Heliyon, 2023;9(12) https://doi.org/10.1016/j.heliyon.2023.e22485 PMid:38076186 PMCid:PMC10709400
Published
Abstract Display: 1088 
PDF Downloads: 1007 PDF Downloads: 44 How to Cite
Issue
Section
Copyright (c) 2025 Anita Dhinwa, Gargi Meena, Ridhi Joshi, Rishikesh Meena
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.