Antiradical and inhibitory activities of hydroethanolic extracts of Phyllanthus amarus and Striga hermonthica on α-amylase
Abstract
Objective: The aim of this study was to evaluate the antiradical and inhibitory activity of whole plant extracts of Phyllanthus amarus and Striga hermonthica on α-amylase.
Methods: The crude 70% (v/v) hydroethanolic extracts obtained were used for the assay of polyphenols, the antiradical test with DPPH, the test for the inhibition of α-amylase with DNS (3,5-dinitrosalicylic). The enzyme extracts were obtained from sorghum malt (Sorghum bicolor).
Results: It appears from this study that the extract of P. amarus was richer in polyphenols than the extract of S. hermonthica. Regarding the antiradical activity, the extract of S. hermonthica showed an IC50 of 339.06 ± 1.43 µg/mL against 77.74 ±2.73 µg/mL for the extract of P. amarus. Regarding the anti α-amylase activity, the extract of P. amarus inhibited IMS127 and KMTC 274 respectively 43.64 ±0.59% and 66.28 ±1.97%. On the other hand, the extract of S. hermonthica presented an inhibition of 3.93 ±0.91% on IMS127 and 14.85 ±1.62% on KMTC 274. The extract of P. amarus presents a good antiradical activity and anti α-amylase compared to S. hermonthica extract. In conclusion, these results partly explain the use of these plants in traditional medicine to treat or relieve people suffering from diabetes.
Keywords : α-amylase, inhibition, polyphenols, antiradical, diabetes
Keywords:
α-amylase, inhibition, polyphenols, antiradical, diabetes
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References
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[26] Khoudali S, Benmenssaoud left D, Essaqui A, Zertoubi M, Azzi M, Benaissa M, Study of the antioxidant activity and anti-corrosion action of the methanolic extract of saw palmetto leaves (Chamaerops humilis L.) from Morocco, Journal of Materials and Environmental Science, 2014; 887-898.
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[28] Ali H, Houghton PJ, Soumyanath A, α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes, with particular reference to Phyllanthus amarus, Journal of Ethnopharmacology, 2006; 449-455. https://doi.org/10.1016/j.jep.2006.04.004
[29] Tamil IG, Dineshkumar B, Nandhakumar M, Senthilkumar M, Mitra A, In vitro study on α-amylase inhibitory activity of an Indian medicinal plant, Phyllanthus amarus, Indian Journal of Pharmacology, 2010; 280-282. https://doi.org/10.4103/0253-7613.70107
[30] Mbagwu HOC, Jackson C, Jackson I, Ekpe G, Eyaekop U, Essien G, Evaluation of the hypoglycemic effect of aqueous extract of Phyllanthus amarus in alloxan-induced diabetic albino rats, International Journal of Pharmaceutical and Biomedical Research, 2011; 158-160.
[2] Favier A, Oxidative stress conceptual and experimental interest in understanding disease mechanisms and therapeutic potential, Chemical news, 2003; 108-115.
[3] WHO World Diabetes Report: Executive Summary, Geneva, 2016.
[4] FID. Report: IDF Diabetes Atlas, 9th _ editing, 2019; 1-176.
[5] Kreatsoulas C, Anand SS, The impact of social determinants on cardiovascular disease. Journal of Cardiology, 2010; 8-13. https://doi.org/10.1016/S0828-282X(10)71075-8
[6] WHO Traditional Medicine Strategy 2002-2005, Geneva, 2002.
[7] Béné K, Camara D, Fofie NBY, Kanga Y, Yapi AB, Yapo YC, Ambe SA, Zirihi G N, Ethnobotanical study of medicinal plants used in the Department of Transua, Zanzan District (Côte d' Ivory), Journal of Animal & Plant Sciences, 2016; 4230-4250. http://www.m.elewa.org/JAPS ;
[8] Patel JR, Tripathi P, Sharma V, Chauhan NS, Dixit VK, Phyllanthus amarus: Ethnomedicinal uses, phytochemistry and pharmacology: A review, Journal of Ethnopharmacology, 2011; 286-313. https://doi.org/10.1016/j.jep.2011.09.040
[9] Guha G, Rajkumar V, Ashok Kumar R, Mathew L, Aqueous extract of Phyllanthus amarus inhibits chromium (VI) -induced toxicity in MDA-MB-435S cells, Food and Chemical Toxicology, 2010; 396-401. https://doi.org/10.1016/j.fct.2009.10.028
[10] Boussim IJ, The Phanerogam parasites of Burkina Faso: Inventory, Taxonomy, Ecology and some aspects of their Biology, Special case of Loranthaceae parasites of Shea, thesis of State Doctorate of Natural Sciences, University of Ouagadougou, 2002; 1-298.
[11] Matou M, Bercion S, Merciris P, Meyssonier N, Fernand D, Marianne-Pepin T, Study of the chemical composition and associated pharmacological potential of Phyllanthus amarus Schum and Thonn. (1827) "Grenn anba fèy", International Symposium on Aromatic and Medicinal Plants (CIPAM) - 9th edition, Cayenne, French Guiana, 2016; 1-10.
[12] Ouédraogo RJ, Evaluation of antioxidant and α-amylase inhibitory activities of potentially antidiabetic plants: Case of Mitragyna inermis (willd) O. Kuntze and Tamarindus indica Linn.. Master's thesis in Applied Biological Sciences, Nazi BONI University, Burkina Faso, 2020; 1-50.
[13] Souhila M, Mustapha K, Nacéra M, Study of the extraction of phenolic compounds from different parts of the artichoke flower (Cynara scolymus L.), Nature & Technology, 2013; 35-40.
[14] Adewale IO, Agumanu NE, Otih-Okoronkwo FI, Comparative studies on α-amylases from malted maize (Zea mays), millet (Euleusina coracana) and Sorghum (Sorghum bicolor), Carbohydrate Polymers, 2006; 71-74. https://doi.org/10.1016/j.carbpol.2006.02.022
[15] Adewale IO, Oladejo A, Properties of the isoforms of α-amylase from kilned and unkilned malted sorghum (Sorghum bicolor), Carbohydrate Polymers, 2009; 105-109. https://doi.org/10.1016/j.carbpol.2008.12.011
[16] Xiao Z, Storms R, Tsang A, A quantitative starch -iodine method for measuring αamylase and glucoamylase activities, Analytical Biochemistry, 2006; 146-148. https://doi.org/10.1016/j.ab.2006.01.036
https://10.1016/j.ab.2006.01.036
[17] Singleton VL, Orthofer R, Lamuela-Raventós RM Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent, In Methods in enzymology, 1999; 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
[18] Brand-Williams W, Cuvelier ME, Berset C, Use of a Free Radical Method to Evaluate Antioxidant Activity, LWT-Food Science and Technology, 1995; 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
[19] Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG, 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Faso honey, as well as their radical scavenging activity, Food Chemistry, 2005; 571-577. https://doi.org/10.1016/j.foodchem.2004.10.006
[20] Thalapaneni NR, Chidambaram KA, Ellappan T, Sabapati ML, Mandal SC, Journal of Complementary and Integrative Medicine, 2008; 1-10.
[21] Jaiswal P, Kumar P, α-amylase inhibitory activity of different extract of bark of Albizia lebbeck (L.) Benth, International Journal of Pharmacy and Pharmaceutical Sciences, 2017; 119-122. https://doi.org/10.22159/ijpps.2017v9i8.19411
[22] N'Guessan K, Kadja B, Zirihi G, Traoré D, Aké-Assi L, Phytochemical screening of some Ivorian medicinal plants used in Krobou country (Agboville, Ivory Coast), Natural Sciences, 2009;1-15.
[23] Kiendrebeogo M, Dijoux-Franca MG, Lamien CE, Meda A, Wouessidjewe D, Nacoulma OG, Acute toxicity and antioxidant property of Striga hermonthica (Del.) Benth (Schrophulariaceae), African Journal of Biotechnology, 2005; 919-922.
[24] Velavan S, Phytochemical techniques - a review, World Journal of Science and Research, 2015; 80-91. https://doi.org/10.1002/rwm3.20270
[25] Yi Z, Yan Y, Liang Y, Zeng B, In vitro antioxidant and antimicrobial activities of Pericarpium citric Reticulatae of a new Citrus Cultivar and its main flavonoid, LWT-Food Science and Technology, 2008; 597-603. https://doi.org/10.1016/j.lwt.2007.04.008
[26] Khoudali S, Benmenssaoud left D, Essaqui A, Zertoubi M, Azzi M, Benaissa M, Study of the antioxidant activity and anti-corrosion action of the methanolic extract of saw palmetto leaves (Chamaerops humilis L.) from Morocco, Journal of Materials and Environmental Science, 2014; 887-898.
[27] Sales PM, Souza PM, Simeoni LA, Magalhães PO, Silveira D, α-Amylase inhibitors: a review of raw material and isolated compound from plant source, Journal of Pharmacy & Pharmaceutical Sciences, 2012; 141-183. https://doi.org/10.18433/J35S3K
[28] Ali H, Houghton PJ, Soumyanath A, α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes, with particular reference to Phyllanthus amarus, Journal of Ethnopharmacology, 2006; 449-455. https://doi.org/10.1016/j.jep.2006.04.004
[29] Tamil IG, Dineshkumar B, Nandhakumar M, Senthilkumar M, Mitra A, In vitro study on α-amylase inhibitory activity of an Indian medicinal plant, Phyllanthus amarus, Indian Journal of Pharmacology, 2010; 280-282. https://doi.org/10.4103/0253-7613.70107
[30] Mbagwu HOC, Jackson C, Jackson I, Ekpe G, Eyaekop U, Essien G, Evaluation of the hypoglycemic effect of aqueous extract of Phyllanthus amarus in alloxan-induced diabetic albino rats, International Journal of Pharmaceutical and Biomedical Research, 2011; 158-160.
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ZANTE AA, SOMDA MB, OUATTARA L, ZOUNGO D, PODA FD, OUEDRAOGO RJ, SANOU Y, KABRE BP, KONATE KA, OUEDRAOGO GA. Antiradical and inhibitory activities of hydroethanolic extracts of Phyllanthus amarus and Striga hermonthica on α-amylase. JDDT [Internet]. 15Jun.2022 [cited 18Apr.2024];12(3-S):150-3. Available from: https://jddtonline.info/index.php/jddt/article/view/5517
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