Inhibition of α-amylase activity of Chamaecrista nigricans (Vahl) Greene (Fabaceae-Caesalpinioideae) and Pseudocedrela kotschyi (Schweinf.) Harms (Meliaceae) water extracts

  • Yacouba SANOU Département de Sciences Biologiques, Unité de Formation et de Recherche en Sciences et Techniques, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso. https://orcid.org/0000-0002-1054-5878
  • Martin Bienvenu SOMDA Centre International de Recherche-Développement sur l’Elevage en zone Subhumide https://orcid.org/0000-0002-9997-2533
  • Lassina OUATTARA Département de Sciences Biologiques, Unité de Formation et de Recherche en Sciences et Techniques, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso. https://orcid.org/0000-0001-5537-5246
  • Flora Anne Claire DRABO Laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso. https://orcid.org/0000-0001-7813-5691
  • Relwendé Justin OUEDRAOGO Département de Sciences Biologiques, Unité de Formation et de Recherche en Sciences et Techniques, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso. https://orcid.org/0000-0002-5036-2144
  • Roland NagTiero MEDA Département de Sciences Biologiques, Unité de Formation et de Recherche en Sciences et Techniques, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso. https://orcid.org/0000-0001-7789-280X
  • Georges Anicet OUEDRAOGO Laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso. https://orcid.org/0000-0002-1588-1379

Abstract

Introduction: Antioxidants and glucosidases inhibitors are interesting in diabetes mellitus prevention and its management. However, conventional drugs have a multitude of unenviable side effects. Thus, research is turning towards herbal remedies, hence this study that was interested in two plants used in the traditional treatment of diabetes in Bobo-Dioulasso. Objective: The aim was to evaluate Chamaecrista (C.) nigricans and Pseudocedrela (P.) kotschyi roots water extracts effect on sorghum α-amylase activity.


Methodology: Total phenolic and flavonoid contents were quantified using Folin-Ciocalteu reagent and aluminum trichloride, respectively. FRAP, DPPH and ABTS methods were used to estimate the extracts antioxidant activity. The α-amylase inhibition tests were carried out using the glucose assay method with dinitrosalicylic acid.


Results: The C. nigricans and P. kotschyi extracts have shown strong antioxidant activities, 7031.52±15 and 8762.35±51 µmol AAE.g-1E respectively, referring to trolox using ABTS method. These extracts have also shown an inhibitory effect of 98.57% and 97.67% respectively on α-amylase activity.


Conclusion/perspectives: These plants have in vitro an interesting anti-amylasic potential. Further investigations will be necessary using a pure enzyme and a wider range of concentration of the extracts in order to determine the kinetic parameters and the fractionation by chromatography to determine the most effective fraction(s). Finally, an in vivo evaluation of the anti-diabetic effect of the extracts could be considered.


Keywords: anti α-amylase, antioxidant, Chamaecrista nigricans, Pseudocedrela kotschyi.

Keywords: anti α-amylase, antioxidant, Chamaecrista nigricans, Pseudocedrela kotschyi

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Author Biographies

Yacouba SANOU, Département de Sciences Biologiques, Unité de Formation et de Recherche en Sciences et Techniques, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Département de Sciences Biologiques, Unité de Formation et de Recherche en Sciences et Techniques, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Martin Bienvenu SOMDA, Centre International de Recherche-Développement sur l’Elevage en zone Subhumide

Institut du Développement Rural, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Lassina OUATTARA, Département de Sciences Biologiques, Unité de Formation et de Recherche en Sciences et Techniques, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Flora Anne Claire DRABO, Laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Relwendé Justin OUEDRAOGO, Département de Sciences Biologiques, Unité de Formation et de Recherche en Sciences et Techniques, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Roland NagTiero MEDA, Département de Sciences Biologiques, Unité de Formation et de Recherche en Sciences et Techniques, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Georges Anicet OUEDRAOGO, Laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

Institut du Développement Rural, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.

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SANOU Y, SOMDA MB, OUATTARA L, DRABO FAC, OUEDRAOGO RJ, MEDA RN, OUEDRAOGO GA. Inhibition of α-amylase activity of Chamaecrista nigricans (Vahl) Greene (Fabaceae-Caesalpinioideae) and Pseudocedrela kotschyi (Schweinf.) Harms (Meliaceae) water extracts. JDDT [Internet]. 15Nov.2021 [cited 1Dec.2021];11(6):15-9. Available from: https://jddtonline.info/index.php/jddt/article/view/5121