Toxicological Studies of Hydroethanolic Leaf Extract of Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) on Wistar Rats
Abstract
Introduction: The impact of aromatic plants used in cooking on health is indisputable.
This study aims to evaluate the in vitro cytotoxicity of Artemia salina, acute and the 28 days subchronic toxicity of X. aethiopica dried fruit used like spice in Togo by oral administration of the extract to female Wistar rats.
Results: The A. salina assay showed that the hydroalcoholic extract of X. aethiopica presented any toxicity. The LC50 values of X. aethiopica on brine shrimp were 0.64 ± 0.13 mg/mL. The acute toxicity study revealed neither behavioral disturbances nor death in rats. The lethal dose (DL50) of this extract is greater than 5000 mg/kg body weight. The results of subchronic toxicity showed no significant change in body and organs weight gain in rats at test doses throughout the treatment period. No changes in haematological parameters were observed apart from a significant increase in platelet number at doses of 500 and 1000 mg/kg. Biochemical parameters such as Urea, Creatinine, Triglycerides, Total cholesterol, Serum glutamic pyruvic transaminase (SGPT), C-reactive protein (CRP) and glycemia were not significantly changed. Serum glutamic oxaloacetic transaminase (SGOT) and Alkaline phosphatase (ALP) activities increased at doses of 500/1000 mg/kg and 1000 mg/kg respectively. Creatine Phosphokinase (CPK) activity decrease at the dose of 500 and 1000 mg/kg. Blood electrolytes showed no significant change. The histological sections showed no organ damage.
Conclusions: Aqueous extract of X. aethiopica did not lead to any adverse effects in rats after acute and subchronic treatment at 500 and 1000 mg/kg doses.
Keywords: Xylopia aethiopica, Cytotoxicity, Toxicity, Wistar rats.
Keywords:
Xylopia aethiopica, Cytotoxicity, Toxicity, Wistar ratsDOI
https://doi.org/10.22270/jddt.v12i1-S.5322References
Fetse JP, Kofie W and Adosraku RK, Ethnopharmacological Importance of Xylopia aethiopica (Dunal) A. Rich (Annonaceae), British Journal of Pharmaceutical Research, 2016; 11(1):1-21. https://doi.org/10.9734/BJPR/2016/24746
Godam ET, Olaniyan OT, Wofuru CD, Orupabo CD, Ordu KS, Gbaranor BK., Dakoru PD, Xylopia aethiopica ethanol seed extract suppresses Cadmium chloride-induced ovary and gonadotropins toxicity in adult female Wistar rats, Jornal Brasileiro Reproduçào Assistada. 2021; 25(2):252-256. https://doi.org/10.5935/1518-0557.20200091
Woode E, Alhassan A, Abaidoo CS, Effect of ethanolic fruit extract of Xylopia aethiopica on reproductive function of male rats, International Journal of Pharmaceutical and Biomedical Research, 2011; 2:161-165.
Mlingi NLV, Nkya S, Tatala SR, Rashid S, Bradbry JH, Recurrence of konzo in southern Tanzania: rehabilitation and prevention using the wetting method, Food and Chemical Toxicology, 2011; 49:673-677. https://doi.org/10.1016/j.fct.2010.09.017
Vandegeer R, Miller RE, Bain M, Gleadow RM, Cavagnaro TR, Drought adversely affects tuber development and nutritional quality of the staple crop cassava (Manihot esculenta Crantz), Functional Plant Biology, 2013; 195-200. https://doi.org/10.1071/FP12179
Karumi Y, Onyeyili PA, Ogugbuaja VO, Identification of active principles of M. balsamina (Balsam Apple) leaf extract, Journal of Medical Sciences, 2004; 4 (3):179-182. https://doi.org/10.3923/jms.2004.179.182
Edeoga HO, Okwu DE, Mbaoble BO, Phytochemical constituents of some Nigerian Medicinal plants, African Journal of Biotechnology, 2005; 4(7): 685-688. https://doi.org/10.5897/AJB2005.000-3127
Tofighi Z, Ghazi saeidi N, Hadjiakhoondi A, Yassa N, Determination of cardiac glycosides and total phenols in different generations of Securigera securidaca suspension culture, Research Journal of Pharmacognosy, 2016; 3(2):25-31.
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL, Brine Shrimp: a convenient general bioassay for active plant constituents, Planta Medica, 1982; 45:31-34. https://doi.org/10.1055/s-2007-971236
Diallo A, Dossou-Yovo KM, Osseni A, Darré T, Bakoma B, Lawson-Evi P, Eklu-Gadégbéku K, Toxicological Studies of Hydroethanolic Leaf Extract of Launaea Taraxacifolia (Willd) Amin Ex C. Jeffrey on Wistar Rats, Journal of Medicinal Food, 2020; 23(12):1323 1327. https://doi.org/10.1089/jmf.2019.0221
Dossou-Yovo KM, Diallo A, Lawson-Evi P, Darré T, Bakoma B, Eklu-Gadégbéku K, Cytotoxicity, Acute, and Subacute Study of Hydroalcoholic Root Extract of Carissa Spinarum L. on Wistar Rats, Journal of Medicinal Food, 2020; 24(7):675-685. https://doi.org/10.1089/jmf.2020.0065
OECD: Organization for Economic Cooperative Development, Guidelines for the Testing of Chemicals /Section 4: Health Effects Test No. 423: Acute Oral toxicity - Acute Toxic Class Method Organization for Economic Cooperation and Development, Paris, France, 2002.
OECD: Organization for Economic Cooperative Development, Repeated dose oral toxicity test method. In OECD Guidelines for testing of chemicals, N°407, Organization for Economic Cooperation and Development, Paris, France, 2008.
Aguoru CU, Pilla C, Olasan JO, Phytochemical screening of Xylopia aethiopica with emphasis on its medicinally active principle, Journal of Medicinal Plants Research. 2016; X.
Ezekwesili CN, Nwodo OFC, Eneh FU, Ogbunugafor HA, Investigation of the chemical composition and biological activity of Xylopia aethiopica Dunal (Annonacae), African Journal of Biotechnology. 2010; 9(43):7352-7356.
Okigbo RN, Ezebo RO, Okoli SI, Phytochemical analysis of Xylopia aethiopica (Dun), Citrus limon (L.) and Allium sativum (L.) extracts and their effect on selected human pathogens, Advancement in Medicinal Plant Research, 2020; 8(2):35-42.
Aglave HR, Phytochemical Screening of leaf extract of Ocimum gratissimum L. Var. Clocimum, International Journal of Botany Studies, 2019; 4 (4):60-63.
Ajila CM, Brar SK, Verma M, Tyagi RD, Godbout S, Valéro JR, Extraction and analysis of polyphenols Recent trends, Critical Reviews in Biotechnology, 2011; 31:227-49. https://doi.org/10.3109/07388551.2010.513677
Ajayi AM, Umukoro S, Ben-Azu B, Adzu B & Ademowo OG, Toxicity and Protective Effect of Phenolic-Enriched Ethylacetate Fraction of Ocimum gratissimum (Linn.) Leaf against Acute Inflammation and Oxidative Stress in Rats, Drug Development Research, 2017; 78(3-4):135-145. https://doi.org/10.1002/ddr.21384
Ekeanyanwu RC, Mgbedo NE, Njoku AT, Safety assessment of essential oils from Xylopia aethiopica (Dunal) fruit from Nigeria, Biomedical Research and Therapy, 2020; 7(7):3865-3876. https://doi.org/10.15419/bmrat.v7i7.616
Ayodele PF, Ore A, Akinloye OA, Median Lethality Dose of Xylopia aethiopica Fruit Ethanol Extract, Journal of Analytical Techniques and Research, 2019; 1 (1):033-036.
Okoduwa SIR, Umar IA, James DB, Inuwa HM, Anti-Diabetic Potential of Ocimum gratissimum Leaf Fractions in Fortified Diet-Fed Streptozotocin Treated Rat Model of Type-2 Diabetes, Medicines (Basel), 2017; 4(4):73. https://doi.org/10.3390/medicines4040073
Assih M, Diallo A, Dougnon V, Metowogo K, Lawson-Evi P, Eklu-Gadegbeku K, Potchoo Y, Toxicological study of Manihot esculenta Crantz (Euphorbiaceae) leaf extracts. British Journal of Medical and Health Research, 2021; 8 (04):1-11. https://doi.org/10.46624/bjmhr.2021.v8.i4.001
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