Toxicity Studies on Kushta Khabasul Hadeed (calcined iron rust) prepared by Classical and Muffle Furnace Methods
Toxicity Studies on Kushta Khabasul Hadeed
Abstract
Background: Though a number of studies has been carried out in terms of safety of Kushta but concerns have been raised about the presence of heavy metals in Unani formulations. Classical method of preparation of KKH and other Kushta preparation in Unani system of medicine has been found to eliminate all such issues from the end product. Hence; the present study was designed to evaluate the acute and sub-acute toxicity study of KKH prepared by Classical and Muffle furnace methods.
Objective: To ascertain the safety profile of Kushta Khabasul Hadeed (KKH) prepared by classical and muffle furnace methods.
Materials and methods:
Both of the samples of KKH i.e. Kushta Khabasul Hadeed (KKHCM) and Kushta Khabasul Hadeed (KKHMFM) prepared by Classical and Muffle Furnace Methods respectively were evaluated for acute and sub-acute toxicity by OECD guidelines 423 and 407 respectively. The starting dose in the acute test was 50 mg/kg and in sub-acute test, it was 134mg, 200 mg and 400 mg/kg b.w. Effect of both of the test samples were assessed for ponderal changes, food and water intake, relative organs weights, hematological, biochemical and histopathological features of various organs.
Results: Both of the samples did not produce any sign of toxicity in acute toxicity study up to the dose level of 2000mg/kg b.w. In sub-acute toxicity study no mortality in any of the test groups was observed. KKHCM at different doses showed a slight decrease in food intake in all the test groups but significant difference (P<0.05) was observed in the test group B as compared to plain control.
Conclusion: KKHMFM was found to be relatively toxic than KKHCM at different dose levels. The study demonstrated that both the Classical and Muffle furnace methods of preparation of Kushta are useful for preparing KKH and also safe at the dose prescribed in Unani literature. However, the Kushta prepared by the classical method was found to be safer than the muffle furnace method.
Keywords:
Classical and Muffle furnace methods, Toxicity, Kushta, Mortality, Metals and mineralsDOI
https://doi.org/10.22270/jddt.v11i3.4835References
Vohora SB, Athar M. Mineral Drugs (Used in Ayurveda and Unani Medicine). First ed. Narosa Publishing House Pvt. Ltd., New Delhi; 2008: 90.
Kabeeruddin HM. Kitab Ut Taklees. Central Council for Research in Unani Medicine (CCRUM), New Delhi: 2011:1–220.
Hafeez HA. Sanatut Taklees. Central Council for Research in Unani Medicine (CCRUM), New Delhi; 2010:1–248.
Ahmad H, Wadud A, Sofi G, Jahan N. Review on Kushta (Calx), a unique dosage form of Traditional systems of medicine. Hippocratic journal of Unani medicine. 2018; 13(1):45-72.
WHO. Research guidelines for evaluating the safety and efficacy of herbal medicines, Manila, Philippines: WHO, Regional Office, Western Pacific Region; 1993.
Anonymous, National formulary of Unani Medicine. Part V, New Delhi: CCRUM; Ministry of Health & Family Welfare, Govt. of India; 2008: 55.
Nadkarni A K. Indian Materia Medica. 7th Ed. Mumbai: Popular prakashan; 2010; 2:62-63.
Anonymous. Qarabadeen Majeedi., Ninth ed. In: Proceedings of the All India Unani Tibbi Conference, New Delhi; 1986: 297–98.
Said HM. Hamdard Pharmacopoeia of Eastern Medicine. Sri Satguru Publications, Indian Books Center, New Delhi, India; 1997: 226-27.
Milman N, Byg KE, Bergholt T, Eriksen L. Side effects of oral iron prophylaxis in pregnancy-Myth or reality. Acta Haematol. 2006; 115:53‑7.
Jeelani C, Shameem I, Begum W, Naveed W. Efficacy of Sufuf Khabsul Hadeed in iron deficiency anemia during pregnancy: A randomized controlled trial, International Journal of Medical and Health Research. 2017; 3(2):50-54.
Akhtar MW, Siddiqui MY, Siddiqui MMH, Khan. Efficacy of Majoon Khabsul Hadeed in Faqruddam ba Sabab-e-Qillat-e-Faulad (iron deficiency/ microcytic- hypochromic anaemia), Indian Journal of Traditional Knowledge. 2011; 10 (2):323-6.
Ali MA, Hamiduddin, Zaigham M, Ikram M, Ranjan R. Pharmaceutico-analytical study of Kushtae Shangarf prepared with Jozbua (Myristica fragrans Houtt.) and Phitkari (alum). J Pharm Bioall Sci 2018; 10:144-58.
Freireich EJ, Gehan EA, Rall DP, Schmidt LH, Skipper HE. Quantitative comparison of toxicity of anti cancer agent in mouse, rat, dog, monkey and men. Cancer Chemotherapy Report. 1968; 50(4):219-44.
OECD Guideline for Testing of Chemicals 2001; 423:1-14.
OECD Guideline for Testing of Chemicals 2008; 407:1-8.
The Gazette of India: Extraordinary, Notification, 170. Guideline for evaluation of Ayurveda, Siddha and Unani drugs and other traditional medicines of India. New Delhi: Dept. of AYUSH, Ministry of Health and Family Welfare, Govt. of India; 2008.
Lipnick RL, Cotruvo JA, Hill RN, Bruce RD, Stitzel KA, Walker AP, et al. Com prison of the up-and-down, conventional LD50, and fixed-dose acute toxicity procedures. Food Chem Toxicol.1995; 33:223-31.
Anonymous. Class 6-toxic and infectious substances. In: UN recommendations on the transport of dangerous goods-model regulations. Part-2. 12th revised edition. Ch. 2.6. Geneva: United Nations Publications; 2001.
Chaudhari SY, Nariya MB, Galib R, Prajapati PK. Acute and sub chronic toxicity study of Tamra Bhasma (incinerated copper) prepared with and without Amritikarana. J Ayurveda Integr Med. 2016; 7:23-29.
Aiyalu R, Ramasamy A. Acute and sub-acute Toxicity study of Aqueous extracts of Canscora heteroclita (L) Gilg in Rodents. Pharmacogn. J. 2016; 8(4):399-410.
Joshi N, Dash MK, Dwivedi L, Khilnani GD. Toxicity study of Lauha Bhasma (calcined iron) in albino rats. Ancient Sci. Life; 2016; 35:159-66.
Szalay B, Tatrai E, Nyiro G, Vezer T, Dura G. Potential toxic effects of iron oxide nanoparticles in in vivo and in vitro experiments. J Appl Toxicol; 2012; 32:446–53.
Singh RK, Kumar S, Aman AK, Kala S, Pradhan U, Shayan M, et al. Crystal structure and magnetic properties studies on nanocrystalline Lauha Bhasma is an Ayurvedic medicine. Int. J Ayurveda Altern Med. 2016; 4:17-22.
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