Physiochemical Evaluation and Determination of Chemical Constituent in Rose Petal (Rosa centifolia)

  • Khushbu Singh Department of pharmacy, Sam Higginbottom University of Agriculture, Technology and Sciences Rewa Road Old Bridge, near to Yamuna, Naini, Prayagraj, Uttar Pradesh 211007
  • Dinesh Patel Department of pharmacy, Sam Higginbottom University of Agriculture, Technology and Sciences Rewa Road Old Bridge, near to Yamuna, Naini, Prayagraj, Uttar Pradesh 211007

Abstract

Objectives: The purpose of the present study was to explore the Pharmacognostic parameters for the standardization of Rosa centifolia Petals. 


Material and Methods: The flowers of Rosa centifolia were authenticated and shade dried. Rosa centifolia petals were collected then macromorphological, physiochemical assessment, and the micrometric study was carried out. The dried powder form of the Rosa centifolia Petals was then extracted with different solvent systems alcoholic, ethyl acetate, and petroleum ether, and their extractive values were calculated. Most of the phytochemicals were found in ethanol and ethyl acetate fractions. Thin Layer chromatography (TLC) of the ethanol, ethyl acetate, and petroleum ether extract was performed for important phytochemicals flavonoids and polyphenol. Flavonoids and phenolics showed their presence in all extracts with one spot in each extract for ethanol, ethyl acetate, and petroleum ether.


Result: The Physicochemical exploration showed values for moisture content, moisture sorption capacity, ash values, and extractive values which are within the limits of World Health Organization standards for the crude drug from medicinal plants. Micromeritic analysis of petal powder reveals good flowability. Ethanolic extractive values were found to be higher when compared to extractive values of, ethyl acetate and petroleum ether. Preliminary Phytochemical examination for the sample indicated the presence of carbohydrates, glycosides, alkaloids, flavonoids, and amino acids. Rf value for flavonoid and phenolic on TLC were found to be for ethanol 0.78 and 0.77, for ethyl acetate 0.81 and 0.78, for petroleum ether 0.81 and 0.78 respectively.


Conclusions: The current research would be useful to supplement the information regarding pharmacognostical characteristics, physicochemical evaluation, micrometric analysis, and phytochemical exploration in the Ayurvedic system of medicine for its identification and medicinal use. 


Keywords: Rosa centifolia, Macromorphological description, Physicochemical evaluation, Phytochemical screening, TLC screening. 

Keywords: Rosa centifolia, Macromorphological description, Physicochemical evaluation, Phytochemical screening, TLC screening

Downloads

Download data is not yet available.

Author Biographies

Khushbu Singh, Department of pharmacy, Sam Higginbottom University of Agriculture, Technology and Sciences Rewa Road Old Bridge, near to Yamuna, Naini, Prayagraj, Uttar Pradesh 211007

Department of pharmacy, Sam Higginbottom University of Agriculture, Technology and Sciences Rewa Road Old Bridge, near to Yamuna, Naini, Prayagraj, Uttar Pradesh 211007

Dinesh Patel, Department of pharmacy, Sam Higginbottom University of Agriculture, Technology and Sciences Rewa Road Old Bridge, near to Yamuna, Naini, Prayagraj, Uttar Pradesh 211007

Department of pharmacy, Sam Higginbottom University of Agriculture, Technology and Sciences Rewa Road Old Bridge, near to Yamuna, Naini, Prayagraj, Uttar Pradesh 211007

References

1. Patel DK, Kumar R, Laloo D, Hemalatha S. Diabetes mellitus: An overview on its pharmacological aspects and reported medicinal plants having antidiabetic activity. Asian Pac J Trop Biomed [Internet]. 2012; 2(5):411–20.
2. Kamtekar S, Keer V, Patil V. Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. J Appl Pharm Sci. 2014; 4(9):61–5.
3. Rafe R. Asian Paci fi c Journal of Tropical Medicine. Asian Pac J Trop Med [Internet]. 2017; 10(10):933–9.
4. Singh MP, Pathak K. Animal models for biological screening of anti-diabetic drugs : An overview. 2015; 5(5):37–48.
5. Lesjak MM, Šibul FS, Anac GT, Beara IN, Mimica-dukic NM. Comparative study of biological activities and phytochemical composition of two rose hips and their preserves : Rosa canina L . and Rosa. 2016; 192:907–14.
6. Fathima SN, Murthy SV. Pharmacognostic study of Rosa damascena petals. 2019; 5(4):779–85.
7. Kamil M, Mishra A, Singh D. In vitro and in vivo antidiabetic effect of extracts of Melia azedarach , Zanthoxylum alatum , and Tanacetum nubigenum Mohammad Faheem Khan a , Arun Kumar Rawat b , Shahnaaz Khatoon c ,. Integr Med Res [Internet]. 2018; 7(2):176–83.
8. Auwal MS, Saka S, Mairiga IA, Sanda KA, Shuaibu A, Ibrahim A. Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica ( Thorn mimosa ). 2014; 5(2):95–100.
9. Joshi A, Bhobe M, Sattarkar A. Research Article Phytochemical investigation of the roots of Grewia microcos Linn . 2013; 5(7):80–7.
10. Phytochemical screening , Total phenolics and Antioxidant Activities of Bark and Leaf extracts of Goniothalamus velutinus ( Airy Shaw ) from Brunei. 2015; (June).
11. Hilal T, Alabri A, Hamood A, Al S, Hossain MA, Weli AM, et al. Comparative study of phytochemical screening , antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. J King Saud Univ - Sci [Internet]. 2014; 26(3):237–43.
12. Pandey A, Tripathi S. Concept of standardization , extraction and pre phytochemical screening strategies for herbal drug. 2014; 2(5):115–9.
13. Iqbal E, Abu K, Lim LBL. Phytochemical screening , total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus ( Airy Shaw ) from Brunei Darussalam. J King Saud Univ - Sci [Internet]. 2015; 27(3):224–32.
14. Stefanovits-b É, Omb A. Total Polyphenol Content and Antioxidant Capacity of Rosehips of Some Rosa Species. 2018; 1–10.
15. Rahimuddin S, Abdulaziz H, Doghaither A. EVALUATION OF THE ANTIOXIDANT ACTIVITIES OF AQUEOUS EXTRACTS OF EVALUATION OF THE ANTIOXIDANT ACTIVITIES OF AQUEOUS. 2017; (March 2019).
16. Sonam M, Singh RP, Pooja S. Phytochemical Screening and TLC Profiling of Various Extracts of Reinwardtia indica. 2017; 9(4):523–7.
17. Patel S, Adhav M. Comparative Phytochemical Screening and Thin Layer Chromatographic Analysis of Ethanolic Leaf Extracts of Morphotypes of Hibiscus Rosa- Sinensis Linn . 2016; 5(10):49–55.
18. Chattopadhyay P, Kumar A, Veer V. Journal of Traditional and Complementary Medicine Isolation and characterization of bioactive components from Mirabilis jalapa L . radix. 2016;6.
19. Al R, Al-sheikh Y, Mateen A, Syed R, Janardhan K, Gupta VC. Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains. Saudi J Biol Sci [Internet]. 2014; 21(2):147–51.
20. Veggi PC, Cavalcanti RN, Meireles MAA. Production of phenolic-rich extracts from Brazilian plants using supercritical and subcritical fluid extraction : Experimental data and economic evaluation. J Food Eng [Internet]. 2014; 131:96–109.
21. Prakasia PP, Nair AS. Pharmacognostic and physicochemical standardization of leaves of Glycosmis pentaphylla ( Retz .) DC. 2016; 5(9):23–30.
22. Hassan LG, Dangoggo SM. Journal of Chemical and Pharmaceutical Research. 2010; 2(5):277–85.
23. Farag RS, Abdel-latif MS, Abd HH, Baky E, Tawfeek LS. Phytochemical screening and antioxidant activity of some medicinal plants ’ crude juices. Biotechnol Reports [Internet]. 2020; 28:e00536.
Statistics
97 Views | 17 Downloads
How to Cite
1.
Singh K, Patel D. Physiochemical Evaluation and Determination of Chemical Constituent in Rose Petal (Rosa centifolia). JDDT [Internet]. 15May2021 [cited 18Jun.2021];11(3):9-6. Available from: https://jddtonline.info/index.php/jddt/article/view/4754