A Comparative Study of Analgesic, Antidiarrhoeal and Antimicrobial Activities of Methanol and Acetone Extracts of Fruits Peels of Limonia acidissima L. (Rutaceae)
Abstract
Objectives: Present study was designed to evaluate Analgesic, Antidiarrhoeal and Antimicrobial activities of methanol and acetone extracts of fruit peels of Limonia acidissima L. by different methods.
Method: The analgesic activity of the samples was studied using acetic acid- induced writhing model in mice. Castor oil-induced antidiarrheal activity was observed by Thomas method and antimicrobial activity was monitored by disc diffusion method.
Results: Limonia acidissima L. inhibited 60.53 % and 59.65 % writhing of methanol as well as acetone fruit peels extracts, respectively, compared to standard drug Diclofenac Na inhibited 78.07 % writhing. At higher dose (500 mg/kg) of the methanol and acetone fruit peels extracts, significant inhibition 47.13 and 44.83 % of characteristic diarrhoeal feces was observed, respectively, as well as at lower dose (250 mg/kg) of the both extracts, inhibition 34.45 and35.63 %. Mention able on average 12mm zone of inhibition was observed of both extract at 250µg/disc and 500µg/disc compare to zone of inhibition 36mm of ciprofloxacin at 50µg/disc.
Conclusion: From the above results, it will be very much possible source for an isolating lead compound for curing the numerous disorders.
Keywords: Limonia acidissima L. Fruit peels, Diclofenac sodium, Loperamide, Ciprofloxacin.
Keywords:
Limonia acidissima L. Fruit peels, Diclofenac sodium, Loperamide, CiprofloxacinDOI
https://doi.org/10.22270/jddt.v10i1-s.3882References
Thomson WA, Schultes RE. Medicines from the Earth. McGraw-Hill; 1978.
Mahesh B, Satish S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World journal of agricultural sciences. 2008 Nov 4; 4(5):839-43.
Wong KC, Wong SN. Volatile constituents of Averrhoa bilimbi L. fruit. Journal of Essential Oil Research. 1995 Nov 1; 7(6):691-3.
Khare CP. Indian medicinal plants: an illustrated dictionary. Springer Science & Business Media; 2008 Apr 22.
Efloraofindia. Limonia acidissima. Available from https://sites.google.com/site/efloraofindia/species/m---z/r/rutaceae/limonia/limonia-acidissima. Accessed 12 March 2019.
Useful tropical plants. Limonia acidissima. Available from http://tropical.theferns.info/viewtropical.php?id=Limonia+acidissima. Accessed 12 March 2019.
Choi EM, Hwang JK. Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia. 2004 Sep 1; 75(6):557-65.
ENCYCLOPAEDIA BRITANNICA. Analgesic drug. Available from https://www.britannica.com/science/analgesic. Accessed 12 March 2019.
Physiopedia. Pharmacology in Pain Management. Available from https://www.physio-pedia.com/Pharmacology_in_Pain_Management. Accessed 12 March 2019.
Hegde MM, Lakshman K, Girija K, KUMAR BA, Lakshmiprasanna V. Assessment of antidiarrhoeal activity of Desmostachya bipinnata L.(Poaceae) root extracts. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 2010; 9(4):312-8.
Maiti A, Dewanjee S, Mandal SC. In vivo evaluation of antidiarrhoeal activity of the seed of Swietenia macrophylla King (Meliaceae). Tropical journal of pharmaceutical research. 2007; 6(2):711-6.
Akter R, Hasan SR, Hossain MM, Jamila M, Chowdhury SS, Mazumder ME, Rahman S. Antidiarrhoeal and antioxidant properties of Curcuma alismatifolia leaves. Australian Journal of Basic and Applied Sciences. 2010 Mar 1; 4(3):450-6.
Srivastava JP, Lambert J, Vietmeyer N. Medicinal plants: An expanding role in development. The World Bank; 1996 Apr 30.
Rauha JP, Remes S, Heinonen M, Hopia A, Kähkönen M, Kujala T, Pihlaja K, Vuorela H, Vuorela P. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. International journal of food microbiology. 2000 May 25; 56(1):3-12.
Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants?. Journal of ethnopharmacology. 1998 Feb 1; 60(1):1-8.
Ahmed F, Selim MS, Das AK, Choudhuri MS. Anti-inflammatory and antinociceptive activities of Lippia nodiflora Linn. Die Pharmazie-An International Journal of Pharmaceutical Sciences. 2004 Apr 1; 59(4):329-30.
Shoba FG, Thomas M. Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhoea. Journal of ethnopharmacology. 2001 Jun 1; 76(1):73-6.
Wiart C, Mogana S, Khalifah S, Mahan M, Ismail S, Buckle M, Narayana AK, Sulaiman M. Antimicrobial screening of plants used for traditional medicine in the state of Perak, Peninsular Malaysia. fitoterapia. 2004 Jan 1; 75(1):68-73.
Medicine Plus. Pain. Available from https://medlineplus.gov/pain.html. Accessed 15 March 2019.
Basbaum AI, Bautista DM, Scherrer G, Julius D. Cellular and molecular mechanisms of pain. Cell. 2009 Oct 16; 139(2):267-84.
PNAS. Mechanisms of Pain. Available from https://www.pnas.org/content/98/21/11845. Accessed 15 March 2019.
Kase Y, Saitoh K, Makino B, Hashimoto K, Ishige A, Komatsu Y. Relationship between the antidiarrhoeal effects of Hange‐Shashin‐To and its active components. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 1999 Sep; 13(6):468-73.
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