Anticancer and Cytotoxic Potential of Aqueous Extract of Triticum aestivum on Colorectal Carcinoma

  • Janki B Patel
  • Piyush Patel Examination Controller, Gujarat University, Navarangpura, Ahmedabad, 380009, Gujarat, India.
  • Rakeshkumar S Parmar Parul Institute of Pharmacy & Research, Faculty of Pharmacy, Parul University, P.O Limda, Ta. Waghodiya- 391760, Dist. Vadodra, Gujarat, India.
  • Pinkal Patel
  • Dhara Patel Parul Institute of Pharmacy & Research, Faculty of Pharmacy, Parul University, P.O Limda, Ta. Waghodiya- 391760, Dist. Vadodra, Gujarat, India.

Abstract

Introduction: Cancer is caused by abnormalities in genetic material of the transformed cells. Cancer may also be initiated by carcinogens, tobacco, smoke, radiation, chemicals or infectious agents, especially some viruses. Cancers cause annually more than 13% of all human deaths. More than 70% of all cancer deaths occurred in low and middle income countries. Deaths from cancer worldwide are projected to continue rising, with an estimated 12 million deaths in 2030 (WHO estimate). Natural products have been used as traditional medicines in many parts of the world like Egypt, China, Greece, and India from ancient times. It is from these medicinal plants, the modern drugs been developed known to be free of the deleterious effects, are inexpensive and effective. One of these herbs is Wheatgrass, the young grass of Triticum aestivum Linn., family: Poeaceae. Objectives: Objective of the study was to analyze anticancer property of leaves of Triticum aestivum on HCT-15 cells. Materials and methods: The young grass of Triticum aestivum is was collected. The aqueous extract was prepared by using standard protocols. The antiproliferative effect of the aqueous extract was evaluated in vitro by employing MTT assay. The potency of plant extract was calculated in terms of percent decrease in viable HCT-15 cells as compared to the control. Result and conclusion: The extract showed dose dependent antitumor activity. The MTT assay showed an anti proliferative activity (IC50) at 258.8 μg/ml of crude extract.


Keywords: Triticum aestivum, CRC, HCT-15, IC50, MTT Assay.

Downloads

Download data is not yet available.

Author Biographies

Janki B Patel

Parul Institute of Pharmacy & Research, Faculty of Pharmacy, Parul University, P.O Limda, Ta. Waghodiya- 391760, Dist. Vadodra, Gujarat, India.

Piyush Patel, Examination Controller, Gujarat University, Navarangpura, Ahmedabad, 380009, Gujarat, India.

Examination Controller, Gujarat University, Navarangpura, Ahmedabad, 380009, Gujarat, India.

Rakeshkumar S Parmar, Parul Institute of Pharmacy & Research, Faculty of Pharmacy, Parul University, P.O Limda, Ta. Waghodiya- 391760, Dist. Vadodra, Gujarat, India.

Parul Institute of Pharmacy & Research, Faculty of Pharmacy, Parul University, P.O Limda, Ta. Waghodiya- 391760, Dist. Vadodra, Gujarat, India.

Pinkal Patel

Parul Institute of Pharmacy & Research, Faculty of Pharmacy, Parul University, P.O Limda, Ta. Waghodiya- 391760, Dist. Vadodra, Gujarat, India.

Dhara Patel, Parul Institute of Pharmacy & Research, Faculty of Pharmacy, Parul University, P.O Limda, Ta. Waghodiya- 391760, Dist. Vadodra, Gujarat, India.

Parul Institute of Pharmacy & Research, Faculty of Pharmacy, Parul University, P.O Limda, Ta. Waghodiya- 391760, Dist. Vadodra, Gujarat, India.

References

1. WHO; IARC (International Agency for Research on Cancer); Latest World Cancer Statistics, Global Cancer Burden Rises to 14.1 Million New Cases in 2012, 12th December 2013 Press Release No. 233. [Last accessed on 2015 Jun 17]. Available from: http://www.iarc.fr/en/mediacentre/pr/2013/pdfs/pr223_E.pdf .
2. GLOBOCAN 2012 Estimated Cancer Incidence, Mortality and Prevalence About Colorectal Carcinoma, Worldwide in 2012. [Last accessed on 2015 Jun 19]. Available from: http://www.globocan.iarc.fr/pages/fact_sheets_cancer.aspx?cancer=colorectal .
3. Ferlay J, Soerjomataram I, Ervik M, et al. GLOBOCAN 2012 v1.0, Cancer Incidence and Mortality Worldwide: IARC Cancer Base No. 11. Lyon, France: International Agency for Research on Cancer, 2013.
4. Modha J, Modha N. International Centre for Ayurveda Studies. Jamnagar, Gujarat, India: Gujarat Ayurveda University; 2007. Role of Ayurveda in the Management of Cancer.
5. Cragg GM, Newman DJ, Snader KM. Natural products in drug discovery and development. Journal of Natural Products, 1997;60:52–60. [PubMed: 9014353].
6. Gupta S, Zhang D, Yi J, Shao J. Anticancer activities of Oldelandia diffusa. Journal of Herbal Pharmacotherapy, 2004;4:21–33. [PubMed: 15273074].
7. Aquil F, Ahmad I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Trukish Journal of Biology, 2006;30:177–83.
8. The wealth of India, Council of Scientific & Industrial research, New Delhi, 10, 312-323.
9. Schnabel C, The biologic value of high protein cereal grasses. Paper presented to the biologic section of the American Chemical Society, New York, April 2, 1940.
10. Laboratory Analyses, Nutrition International, East Brunswick, NJ, Sep 6, 1989.
11. Hamilton E, Whitney E, Sizer F, Nutrition: Concepts and Controversies. West Publishing Co., St. Paul, Minn, 4th ed, 1988.
12. Kohler G, The unidentified vitamins of grass and alfalfa, Feedstuffs, Aug. 8, 1953.
13. Padalia S et al., Multitude potential of wheatgrass juice (Green Blood) An Overview, Chronicles of young scientists 2011;1: 23-24.
14. Lai CN, Chlorophyll: the active factor in wheat sprout extracts inhibiting the metabolic activation of carcinogens in vitro, Nutrition and Cancer, 1979; 1: 19–21.
15. Lai CN, Dabney BJ, Shaw CR, Inhibition of in vitro metabolic activation of carcinogens by wheat sprout extracts. Nutrition and Cancer 1978; 1: 27–30.
16. Falcioni G, Fedeli D, Tiano I, Antioxidant activity of wheat sprout extracts in vitro: Inhibition of DNA oxidative damage, Journal of Food Science, 2002; 67: 2918-2922.
17. Wigmore A, The wheatgrass Book, Avery Publishing Group, Wayne, New Jersey, 1985.
18. Marwaha R et al., Wheat Grass Juice Reduces Transfusion Requirement in Patients with Thalassemia Major: A Pilot Study, Indian Pediatrics, 2004, 41.
19. Ben-Arye E et al., Wheatgrass juice in the treatment of active distal ulcerative colitis: a random double-blind placebo-controlled trial, Scand Journal of Gastroenterology, 2002; 37: 444–449.
20. Yasumura Y, Kawakita M, The research for the SV40 by means of tissue culture technique, Nippon Rinsho, 1963; 21(6): 1201–1219.
21. Shimizu B, In Seno K, Koyama H, Kuroki T, Manual of selected cultured cell lines for bioscience and biotechnology (in Japanese), Tokyo, Kyoritsu Shuppan, 1993, 299–300.
22. Ammerman NC, Magda Beier-Sexton, Abdu FA, Growth and Maintenance of Vero Cell Lines, Current Protocol Microbiology, 2008: 1-10.
23. Percival J, In: The wheat plant, Duckworth, U K, 1974, 55-59.
24. Freshney RI, Animal cell culture- a practical approach,2002.
25. Mosmann T, Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays, Journal of Immunology Methods, 1983; 65: 55-63.
26. Wilson AP, Cytotoxicity and Viability Assays in Animal Cell Culture: A Practical Approach, 3rd Ed, Oxford University Press, Oxford, 2000, 1.
27. Patel S, Gheewala N, Suthar A, Shah A, In- vitro cytotoxicity activity of Solanum Nigrum extract against Hela cell line. International journal of pharmacy and pharmaceutical sciences, 2009; 1(1): 38-46.
28. Berridge MV, Herst PM, Tan AS, Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. In: El-Gewely MR, editor, Biotechnology Annual Review. Amsterdam, Elsevier; 2005, 127–152.
29. Mantani N, Imanishi N, Kawamata, H, Terasawa K, Ochiai H, Inhibitory effect of (+)-catechin on the growth of influenza A/PR/8 virus in MDCK cells. Planta Medica, 2001; 67: 240‐43.
30. Jack D Burton, The MTT assay to evaluate chemosensitivity. Chemosensitivity, Humana Press Inc. Totowa, NJ, 2005m 1: 69‐77.
31. Quintero A, Pelcastre A, Solano JD, Cytotoxic activity of crude extracts from Astragalus chrysochlorus (Leguminosae). Journal of Pharmacy and Pharmaceutical Science, 1999; 2: 108-112.
32. Taylor RSL, Manendhar NP, Hudson JB, Towers GHN, Antiviral activities of Nepalese medicinal plants, Journal of Ethnopharmacology, 1996; 52: 157‐63.
33. Vlietink AJ, Van Hoof L, Totte J, Lasure A, Vanden Berghe D, Rwangabo PC, Mvukiyumwani J, Screening of hundred Rwandese medicinal plants for antimicrobial and antiviral properties. Journal of Ethnopharmacology, 1995; 46: 31‐47.
34. Jaiprakash B, Chandramohan D, Reddy N, Burn wound healing activity of Euphorbia hirta, Ancient Science of Life, 2006; 25(3&4): 1‐3.
35. Liu Y, Murakami N, Ji H, Abreu Pedro and Zhang S, Antimalarial flavonol glycosides from Euphorbia hirta, Pharmaceutical Biology, 2007; 45: 278‐81.
Crossmark
Statistics
454 Views | 417 Downloads
How to Cite
1.
Patel JB, Patel P, Parmar RS, Patel P, Patel D. Anticancer and Cytotoxic Potential of Aqueous Extract of Triticum aestivum on Colorectal Carcinoma. JDDT [Internet]. 7Jul.2019 [cited 28Mar.2024];9(4):164-9. Available from: https://jddtonline.info/index.php/jddt/article/view/3075