Simultaneous Estimation & Validation of Praziquantel & Pyrantel Pamoate in Bulk & Pharmaceutical Dosage Form by Using RP-HPLC

Authors

  • Nachiket S. Dighe Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.- Ahmednagar, 413736, India (MS)
  • Ganesh S. Shinde Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.- Ahmednagar, 413736, India (MS)
  • K .V. Dhamak Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.- Ahmednagar, 413736, India (MS)
  • R.G. Shete Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.- Ahmednagar, 413736, India (MS)

Abstract

The first reversed phase high performance liquid chromatographic method for quantitation studies  of, Praziquantel and Pyrantel Pamoate has been developed and validated to be a simple, sensitive, rapid, specific, precise, and accurate method. Chromatographic separation was achieved on C18 column (250×4.6 mm-5μm p.s). Methanol and water in a ratio [85:15 v/v] as a mobile phase at flow rate of 0.8ml/min. UV detection was operated at 217 nm and injection volum was 20 μl.. The proposed method showed good linearity, accuracy, precision and was successfully applied for determination of the drugs in laboratory prepared pharmaceutical dosage forms. The current method has been statistically validated according to the ICH guidelines and this method has been subsequently developed and applied successfully to determine the levels of Praziquantel and Pyrantel pamoate in a combined formulation and in the routine quality control analysis with good accuracy and sensitivity.

Keywords: Praziquantel, Pyrantel pamoate, Quantitation Studies,   RP-HPLC ,

DOI

https://doi.org/10.22270/jddt.v9i3-s.2951

Author Biographies

Nachiket S. Dighe, Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.- Ahmednagar, 413736, India (MS)

Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.-  Ahmednagar, 413736, India (MS)

Ganesh S. Shinde, Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.- Ahmednagar, 413736, India (MS)

Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.-  Ahmednagar, 413736, India (MS)

K .V. Dhamak, Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.- Ahmednagar, 413736, India (MS)

Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.-  Ahmednagar, 413736, India (MS)

R.G. Shete, Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.- Ahmednagar, 413736, India (MS)

Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar. Tal-Rahata, Dist.-  Ahmednagar, 413736, India (MS)

Published

20-06-2019
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How to Cite

1.
Dighe NS, Shinde GS, Dhamak K .V., Shete R. Simultaneous Estimation & Validation of Praziquantel & Pyrantel Pamoate in Bulk & Pharmaceutical Dosage Form by Using RP-HPLC. J. Drug Delivery Ther. [Internet]. 2019 Jun. 20 [cited 2025 Mar. 18];9(3-s):688-92. Available from: https://jddtonline.info/index.php/jddt/article/view/2951

Issue

Vol. 9 No. 3-s (2019): Volume 9, Issue 3-s, May-June 2019 (Supplement Issue)

Section

Research

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How to Cite

1.
Dighe NS, Shinde GS, Dhamak K .V., Shete R. Simultaneous Estimation & Validation of Praziquantel & Pyrantel Pamoate in Bulk & Pharmaceutical Dosage Form by Using RP-HPLC. J. Drug Delivery Ther. [Internet]. 2019 Jun. 20 [cited 2025 Mar. 18];9(3-s):688-92. Available from: https://jddtonline.info/index.php/jddt/article/view/2951
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