Comparative analysis of different identification techniques for detection of mycobacterial species in clinical samples
Abstract
Out of 906 cultures isolates, 263 (29.0%) were confirmed as NTM and 724 (71.0%) were identified as Mycobacterium tuberculosis complex. The diversity of NTM species was high and predominated by Mycobacterium abscessus (31.3%) followed by M. fortuitum (22%), M. intracellulare (13.6%), M. chelonae (9.1%), however, M. abscessus and M. fortuitum were the predominant species in both types of clinical isolates. Men (60.4%) and older patients aged greater than 55 years were the predominated risk group for NTM infection. Additionally, three HIV/AIDS cases (1.1%), as well as thirty five cases were found to be type two diabetes mellitus infected with non-tuberculous bacteria.
Keywords: NTM, Technique, BacT/Alert, Biochemical Test, Tuberculosis.
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References
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18. Henry M, Nontuberculous mycobacteria in non‐HIV patients: epidemiology, treatment and response, European Respiratory Journal, 2004; 23:741-746.
2. Gupta P, A preliminary report on characterization and identification of non tuberculous mycobacteria (NTM) on the basis of biochemical tests and protein/isoenzyme electrophoretic patterns, Indian Journal of Medical Microbiology, 2002; 20:137.
3. Ferreira RMC, Non-tuberculous mycobacteria I: one year clinical isolates identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in pre highly active antiretroviral therapy era, Memórias do Instituto Oswaldo Cruz, 2002; 97:725-729.
4. Hoefsloot W, The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study, European Respiratory Journal, 2013; 42:1604-1613.
5. Morimoto K, A steady increase in nontuberculous mycobacteriosis mortality and estimated prevalence in Japan, Annals of the American Thoracic Society, 2014; 11:1-8.
6. Henry M, Nontuberculous mycobacteria in non‐HIV patients: epidemiology, treatment and response, European Respiratory Journal, 2004; 23:741-746.
7. Nasiri MJ, Prevalence of non-tuberculosis mycobacterial infections among tuberculosis suspects in Iran: systematic review and meta-analysis, PLoS ONE, 2015; 10:e0129073.
8. Lai CC, Increasing incidence of nontuberculous mycobacteria, Taiwan, 2000–2008, Emerging Infectious Diseases, 2010; 16:294.
9. Ding L, Disease caused by non-tuberculous mycobacteria in a university hospital in Taiwan, 1997–2003, Epidemiology & Infection, 2006; 134:1060-1067.
10. Zheng C, C Fanta, Non-tuberculous mycobacterial pulmonary infection in the immunocompetent host, QJM: An International Journal of Medicine, 2013; 106:307-315.
11. Kendall BA, Winthrop KL, Update on the epidemiology of pulmonary nontuberculous mycobacterial infections, In Seminars in Respiratory and Critical Care Medicine, 2013:087-094.
12. Hoffmann C, Disclosure of the mycobacterial outer membrane: cryo-electron tomography and vitreous sections reveal the lipid bilayer structure, Proceedings of the National Academy of Sciences, 2008, 105:3963-3967.
13. Schulze-Röbbecke R, Buchholtz K, Heat susceptibility of aquatic mycobacteria, Applied and Environmental Microbiology, 1992, 58:1869-1873.
14. Hoffmann C, Disclosure of the mycobacterial outer membrane: cryo-electron tomography and vitreous sections reveal the lipid bilayer structure, Proceedings of the National Academy of Sciences, 2008; 105:3963-3967.
15. Schulze-Röbbecke R, Buchholtz K, Heat susceptibility of aquatic mycobacteria, Applied and Environmental Microbiology, 1992; 58:1869-1873.
16. Falkinham Iii J, Surrounded by mycobacteria: nontuberculous mycobacteria in the human environment, Journal of Applied Microbiology, 2009; 107:356-367.
17. Morimoto K, A steady increase in nontuberculous mycobacteriosis mortality and estimated prevalence in Japan, Annals of the American Thoracic Society, 2014; 11:1-8.
18. Henry M, Nontuberculous mycobacteria in non‐HIV patients: epidemiology, treatment and response, European Respiratory Journal, 2004; 23:741-746.
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Umrao J, Sivaperumal R. Comparative analysis of different identification techniques for detection of mycobacterial species in clinical samples. JDDT [Internet]. 15Dec.2018 [cited 17Apr.2024];8(6-s):290-3. Available from: https://jddtonline.info/index.php/jddt/article/view/2137
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