2D-Molecular homology modeling of selected Enolase enzyme for Leishmaniasis
Abstract
In present study, BLAST search was performed and an identity with Trypanosoma brueei is 79% Based upon and Pfam results Enolase- 2PTZ (protein data bank) was considered as an ideal homologue and used as a template for homology modeling due to its higher X-ray resolution at 1.65. Sequence alignment between enolase and 2PTZ was done using align 123 followed by manual modification. The final alignment was carefully evaluated and evidenced to be matching the conserved residue data for chain A of enolase in Trypanosoma brucei well Superimposing of the model was done over the template 2PTZ. Further research on the comparison of the models for the inhibitors may eluciadate the mechanism of enolase ligands interactions. This study has shown that some formation of favorable hydrogen bonds, hence it is predicated from this study that these novel compounds may act as potent inhibitors for Leishmania Enolase. These compounds may be used further for synthesis, their wet lab activity against Enolase, animal model study and clinical trials and then can be implicated for the treatment of leishmaniasis. Those models are considered to be used in designing new leads for hopefully more active compounds.
Keywords: Enolase, Leishmaniasis, Enzyme, 2D-moleculare Modeling
DOI
https://doi.org/10.22270/jddt.v8i6-s.2117References
Mauricio I. L., Stothard J. R., Miles M. A., The strange case of Leishmania chagasi, Parasitol. Today. 2000; 16:188-189.
Alvar J., Canavate C., Molina R., Moreno J., Nieto J., Leishmaniasis. Adv. Parasitol. 2004; 57:1-88.
Wolday D., Berhe N., Akuffo H., Desjeus P.,9 Britton S., Emerging Leishmania/HIV co-infection in Africa. Medical Microbiology and Immunology (Berlin). 2001; 190:65-67.
Seaman J., Mercer A. J., Sondorp E., The epidemic of visceral leishmaniasis in western Uppernile, southern Sudan course and impact from 1984 to 1994. Int. J. Epidemiol. 1996; 25:862-871.
Alvar J., Yactayo S., Bern C, Leishmaniasis and poverty. Trends Parasitol., 2006; 22:552-557.
www.uni-tuebingen.de/..Mode_Leish_Cycle_en.html.
Killick-Kendrick R., Molineus W., Transmission of leishmaniasis by thebite of a phlebotomine, possible mechanisms. Trans R. Soc Trop Med. Hyg, 1981; 75:152-154.
Francois, S., Sundar, s., Hailu, A., Ghalib, H., Rija, S., Rosanna, W. P., Alvar, J., and Boelaet, M., Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nature Review, Microbiology, 2007; 5:873-882,
http://www.niaid.nih.gov/topics/leishmaniasis/pages/lifecycle.aspx
www.nlm.nih.gov/medlineplus/ency/article/001386.
Marleen Boelaert, Sujit Bhattacharya, Francois Chappuis, Sayda H. EI Safi, Asrat Hailu, Dinesh Mondal, Suman Rijal, Shyam Sundar, Monique Wasunna & Rosanna W. Peeling. Evaluation of rapid diagnostic tests: visceral leishmaniasis, Nature Reviews Microbiology, 2007; S30-S39.
Desjeux P., Leishmaniasis; Current situation and new perspectives, comparative immunology, microbiology & infectious diseases, 2004; 27(5):305-318.
Webster’s New International Dictionary of the English Language, 1937, Merriam Company, Springfield, Mass.
Romano AH, Conway T., Evolution of carbohydrate metabolic pathways. Res Microbiol. 1996; 147(6-7):448-55.
Pancholi V, Multifunctional a-enolase: Its role in disease, Cell Mol Life Sci. 2001; 58(7):902-20.
Hoorn RK, Flickweert JP, Staal GE, Purification and properties of enolase of human erythroctyes, Int J Biochem 1974; 5:845-52.
Verlinde C. L., Hannaert V., Blonski C., Willson M., Perie J.J., Fothergill-Gilmore L.A., Opperdoes F. R., Gelb M.H., Hol W. G., Michles P.A., Glycolysis as a target for the design of new anti-trypansome drugs, Drug Resist Updat, 2001; 4(1):50-65.
Published
Abstract Display: 231 
PDF Downloads: 388 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.