SCREENING AND BIOSYTNTHESIS OF FIBRINOLYTIC ENZYME FROM ASPERGILLUS JAPONICUM
Abstract
Fibrin is a protein that forms in the blood clots after trauma or injury. This is essential to stop blood loss. There are more than twenty enzymes in the body that assist in clotting of the blood, while only one that can break the clot down. It is an endogenously produced fibrinolytic enzyme called plasmin. Streptokinase is an extracellular metallo-enzyme produced by beta-haemolytic streptococcus and is used as an effective and cheap clot-dissolving medication in some cases of myocardial infarction (heart attack) and pulmonary embolism. It belongs to a group of medications known as fibrinolytics. Fibrinolytic enzymes can be found in a variety of foods, such as Japanese Natto, Tofuyo, Korean Chungkook-Jang soy sauce, and edible honey mushroom. Fibrinolytic enzymes producing Aspergillus japonicum KSS 05 strain were screened for the production by fibrin plate assay method. The maximum zone of fibrin hydrolysis were found 6 mm diameter. Further the Aspergillus japonicum KSS 05 were employed for the production by submerged fermentation and it showed 235 IU by pH 6, temperature 300C and 1 ml inoculums size.
Key words: Fermentation kinetics, effect of pH, fibrinolytic enzymes and fibrin
Â
Keywords:
diabetes mellitus, efficacy, gliclazide, glimepiride, safety, tolerabilityDOI
https://doi.org/10.22270/jddt.v5i6.1106References
Fujita M, Nomura K, Hong K, Ito Y, Asada A, Nishimuro S. Purification and character of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in japan. BiochemBiophys Res Commun, 1993; 197: 1340-47.
Jeong YK, Yang WS, Kim KH, Chung KT, Loo WH, Park JU. Purification of a fibrinolytic enzyme (myulchikinase) from pickled anchovy and its cytotoxicity to the tumer cell lines. BiotechnolLett, 2004; 26:393-97.
Sumi H, Nakajima N, Yatagai C. A unique strong fibrinolytic enzyme (katsuwokinase) in skipjack Shiokara a Japanese traditional fermented food. Com BiochemPhysiolBiochem MolBiol, 1995; 112:543-47.
Voet D, Voet JG, Charlotte W. Principles of Biochemistry. 3rd ed., Wiley, New York;2008, pp. 87-95.
Kim, S.H. and N.S. Choi, 2000. Purification and characterization of subtilisin DJ-4 secreted by Bacillus sp strain DJ-4 screened from Doen-Jang. Biosci. Biotechnol. Biochem., 64: 1722-1725.
Mine, Y., A.H.K. Wong and B. Jiang, 2005. Fibrinolytic enzymes in Asian traditional fermented foods, Food Research International, 38: 243-250.
Takeno, T., T. Okamura, M. Sera, M. Takana, S. Fukuda and M. Ohsugi, 1999. Screening of fibrinolytic enzymes of microorganisms. The Bulletin of Mukogawa's University Natural of Science, 47: 67-72.
Yu, R., H. Qi, T. Zhang and W.T. Wu, 2005. Preliminary studies on in vitro and in vivo thrombolytic activities of thrombolytic enzyme from an induced Bacillus subtilis strain. Sichuan Da Xue Xue Bao Yi Xue Ban, 36: 93-96.
Choi, N.S., J.J. Song, D.M. Chung, Y.J. Kim, P.J. Maeng and S.H. Kim, 2009. Purification and characterization of a novel thermoacid-stable fibrinolytic enzyme from Staphylococcus sp. strain AJ isolated from Korean salt-fermented Anchovy-joet. J. Ind. Microbiol. Biotechnol., 36: 417- 426.
Egorov, N.S., N.S. Landau and I.I.Milovanova, 1982. Fibrinolytic activity in mono- and mixed cultures of coryneform bacteria. Nauchnye Doki Vyss Shkoly Biol Nauki, 10: 86-90.
Imshenetskii, A.A., N.S. Demina, S.V. Lysenko and M.D. Evdokimova, 1991. Fibrinolytic activity of bacteria from Pseudomonas genus. Prikl. Biokhim. Mikrobiol., 27: 845-849.
Demina, N.S., F. Veslopolova and G.P. Gaenko, 1990. The marine bacterium Alteromonas piscicida-a producer of enzymes with thrombolytic action. Izv. Akad. Nauk. SSSR Biol., 3: 415-419.
Batomunkueva, B.P. and N.S. Egorov, 2001. Isolation, purification and resolution of the extracellular proteinase complex of Aspergillus ochraceus 513 with fibrinolytic and anticoagulant activities. Microbiology, 70: 519-522.
Wu, B., L. Wu, D. Chen, Z. Yang and M. Luo, 2009. Purification and characterization of a novel fibrinolytic protease from Fusarium sp. CPCC 480097. J. Ind. Microbiol. Biotechnol., 36: 451-459.
Liu, X.L., L.X. Du, F.P. Lu, X.Q. Zheng and J. Xiao, 2005. Purification and characterization of a novel fibrinolytic enzyme from Rhizopus chinensis12. Appl. Microbiol. Biotechnol., 67: 209–214.
EI-Aassar, S.A., H.M. EI-Badry and A.F. Abdel-Fattah, 1990. The biosynthesis of proteases with fibrinolytic activity in immobilized cultures of Penicillium chrysogenum H9. Appl. Microbiol. Biotechnol., 33: 26-30.
Kim, J.S., S. Kumar, S.E. Park, B.S. Choi, S. Kim, T.H. Nguyen, C.S. Kim, H.S. Choi, M.K. Kim, H.S. Chun,Y. Park and S.J. Kim, 2006. A fibrinolytic enzyme from the medicinal mushroom Cordyceps militaris. J. Microbiol., 44: 622-631.
Pandee, P., A.H. Kittikul, O. Masahiro and Y. Dissara. 2008. Production and properties of a fibrinolytic enzyme by Schizophyllum commune BL23. Songklanakarin J. Sci. Technol., 30: 447-453.
Cha, W.S., S.S. Park, S.J. Kim and D. Choi, 2010. Biochemical and enzymatic properties of a fibrinolytic enzyme from Pleurotus eryngii cultivated under solid-state conditions using corn cob. Bioresour. Technol., 101: 6475-6481.
Astrup T, Mullertz S. The fibrin plate method for estimating fibrinolytic activity. Arch Biochem 1952; 40 : 346-51
Mukesh Kumar D J; Rakshitha R;. Annu M;.Vidhya P. Sharon Jennifer, Sandip Prasad, M. Ravi Kumar and P.T. Kalaichelvan. 2013. Pakistan Journal of Biological sciences,17 (4):529-234.
Shilpa H. K, Jeevan G. Ambekar, Siddalingeshwara K. G and Nilima N. Dongre. 2014. An approach on biosynthesis of fibrinolytic enzyme from Aspergillus Sp. Journal of Chemical and Pharmaceutical Research, 6(12): 797-801
Usama F Ali. ZM and Ibrahim, Journal of Applied Sciences Research, 2008,4(7), 892-899.
Venkatanagaraju E and Divakar G. 2013. International Journal of Pharma Sciences and Research,Vol 4, No 5, 81- 93.
Published
Abstract Display: 616 
PDF Downloads: 857 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.