Antioxidant Activity of Centaurium erythraea Extracts
Phototherapy has known a great evolution all the world and some medicinal plants are importance remedies of some diseases. Centaurium erythraea is one of the medicinal plants widely used in this field. In this study, we have evaluated the total polyphenolic and the flavonoid contents of the methanolic (ME) and aqueous (AqE) extracts of the plant Centaurium erythraea as well as its antioxidant activity using the DPPH (2'2-diphenylpicrylhydrazyl), β-carotene /linoleic acid bleaching and reducing power assays. The total polyphenolic content of the methanolic extract was 35,45± 0,041 μg AGE/mg extract and flavonoids was 6,65 ± 0,060 μg QE/mg. The total polyphenolic content of the aqueous extract was 54,27 ± 0,023 μg AGE/mg extract and flavonoids was 3,275 ± 0,003 μg QE/mg. The results of the DPPH test showed a powerful antioxidant activity with a very similar IC50 for the methanolic (IC50=0.232 ± 0.002mg/ml) and aqueous (0.208 ± 0.002mg/ml) extracts. The inhibitory activity of the tow extracts in the β-carotene/linoleic acid assay was (86.781 ± 0.17%) for the methanolic extract and (77.816 ± 0.69%) for the aqueous extract. ME has the higher reducing power (IC50 = 0.35 ± 0.066 mg / ml) compared to the AqE (IC50 = 1.31 ± 0.047 mg / ml).
Keywords: Centaurium erythraea, antioxidant activity, polyphenols, DPPH scavenging, β-carotene, reducing power.
2. Yildrim A., Oktay M. and Bilaloglu V. The antioxidant activity of the leaves of Cydonia vulgaris. Turkish J. Med. Sc. 2001; 31:23-27.
3. Gulcin, I., Oktay, M., Kufrevioglu, I. O. and Aslan, A. Determination of antioxidant activity of Lichen Cetraria islandica (L) Ach. J. Ethnopharmacol. 2002; 79:325-329.
4. Adeolu A. A., Florence O. J., Anthony J. A. and Patrick J. M. Antioxidant Properties of the Methanol Extracts of the Leaves and Stems of Celtis africana. Rec. Nat. Prod. 2009; 3:23-31.
5. Ansari A. Q., Syed Abrar A., Waheed, M. A. and Sayyed, J. A. Extraction and determination of antioxidant activity of Withania somnifera Dunal. Euro. J. Exp. Bio. 2013; 3(5):502-507.
6. Emrobowansan M. I., Patrick J. M. and Voster M. A Report on the In Vitro Antioxidant Properties of Vachellia karroo Leaf Extract: A Plant Widely Grazed by Goats in the Central Eastern Cape of South Africa. Sustainability. 2017; 164 :1-9.
7. Kumarasamy Y., Nahar L., Cox, P.J., Jaspars M., Sarker S.D. Bioactivity of secoiridoid glycosides from Centaurium erythraea. Phytomedicine. 2003; 10:344–347.
8. Subotic´ A., Jankovic T., Jevremovic S. and Grubisic D. Plant tissue culture and secondary metabolites productions of Centaurium erythraea Rafn., a medical plant. In: Teixeira da Silva JA (ed) Floriculture ornamental and plant biotechnology: advances and topical issues. 1st edn. Global Science Books, London, 2006; 564–570.
9. Schimmer O. and Mauthner H. Polymethoxylated xanthones from the herb of Centaurium erythraea with strong antimutagenic properties in Salmonella typhimurium. Planta Med. 1996; 62:561–564.
10. Mroueh M., Saab Y. and Rizkallah R. 2004. Hepatoprotective activity of Centaurium erythraea on acetaminophen-induced hepatotoxicity in rats. Phytother Res. 18, 431–433.
11. Tuluce Y., Ozkol H., Koyuncu I. and Ine H. Gastroprotective effect of small centaury (Centaurium erythraea L.) on aspirin-induced gastric damage in rats. Toxicol Ind Health. 2011; 27:760–768.
12. Siler B., Zivkovic S., Banjanac T., Cvetkovic J., Nestorovic Z ivkovic J., Ciric A., Sokovic M. and Misic D. Centauries as underestimated food additives: antioxidant and antimicrobial potential. Food Chem. 2014; 147:367–376.
13. Li H.B., Cheng K.W., Wong C.C., Fan K.W., Chen F. and Jiang Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chemistry. 2007; 102:771-776.
14. Bahorun T., Gressier B., Trotin F., Brunete C., Dine T., Vasseur J., Gazin J.C., Pinkas M., Luycky M. and Gazin M. Oxygen species scavenging activity of phenolic extract from Hawthorn fresh plant organs and pharmaceutical preparation. Drug Research. 1996; 16.
15. Burits M. and Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research. 2000; 14:323-328.
16. Kartal N., Sokmen M., Tepe B., Daferera D., Polissiou M. and Sokmen A. Investigation of the antioxidant properties of Ferula orientalis L. using a suitable extraction procedure. Food chemistry. 2007; 100:584–589.
17. Jayaprakash G.K., Singh R.P. and Sakariah K.K. Antioxidant activity of grape seed extracts on peroxidation models in-vitro, J.Agric Food Chem. 2001; 55:1018-1022.
18. Choi Y., Jeong H. and Lee J. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chemistry. 2007; 103:130-138.
19. Naczk M. and Shahidi F. Extraction and analysis of phenolics in food. Journal of Chromatography A, 2004; 1054:95-111.
20. Abdeldaiem M. H., Mohamed H. G. and Abdel-Khalek H. H. Antimicrobial and Antioxidant Activities of Rosemary Essential Oil Treated By Gamma Irradiation. J. Rad. Res. Appl. Sci. 2009; 2:819-837.
21. Othman A., Ismail A., Abdul Ghani N. and Adenan I. Antioxidant capacity and phenolic content of cocoa beans. Food Chemistry. 2007; 100:1523-1530.
22. Kulistic T., Radonic A., Katalinic V. and Milos M. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chemistry. 2004; 85:633-640.
23. Labiad M.H., Harhar H., Ghanimi A. and Tabyaoui, M. Phytochemical Screening and Antioxidant Activity of Moroccan Thymus satureioïdes Extracts. JMES. 2017; 8:2132-2139.
24. Valentão P., Fernandes E., Carvalho F., Andrade P. B., Seabra R. M. and Bastos M. L. Hydroxyl radical and hypochlorous acid scavenging activity of small Centaury (Centaurium erythraea) infusion: a comparative study with green tea (Camellia sinensis). Phytomedicine, 2003; 10:517-522.
25. Valentao P., Femandes E., Carvalho F., Andrade P. B., Seabra R. M., and Bastos M. L. Antioxidant activity of Centaurium erythraea infusion evidenced by its Superoxide radical scavenging and xanthine oxidase inhibitory activity.J. Agric. Food Chem., 2001; 49:3476–3479.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).