Acute Toxicity Study of Aqueous Extracts of Thymelaea hirsuta Leaves And Flowers in Rabbits
Abstract
Thymelaea hirsuta is a medicinal plant known for its therapeutic virtues in traditional medicine. The objective of this study was to evaluate the acute toxicity of aqueous extract of leaves and flowers of Thymelaea hirsuta in rabbit. Aqueous extract was administered to rabbit in single doses of 2.5, 4, 5.5 and 7 g/kg given by gavage. General behavior, adverse effects and mortality were determined for up to 7 days. Biochemical parameters aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), cholesterol (CHOL) and glucose (GLU) were determined after 7 days. The results showed no mortality was observed during the 7 days of the observation period, an increase in AST and ALT in treated groups at the doses of 4, 5.5 and 7 g/kg, and a decrease in both cholesterol and glucose levels in treated groups compared to control.
Keywords: Thymelaea hirsuta, Acute toxicity, Biochemical parameters, adverses effects.
Keywords:
Thymelaea hirsuta, Acute toxicity, Biochemical parameters, adverses effectsDOI
https://doi.org/10.22270/jddt.v10i4.4141References
Verma SK and Kumar A. Therapeutic uses of Withania somnifera (Ashwagandha) with a note on withanolides and its pharmacological actions. Asian Journal of Pharmaceutical and Clinical Research. 2011; 4:1-4.
Gesler WM. Therapeutic landscapes: medicinal issues in light of the new cultural geography. Social Sci Med. 1992; 34:735-746.
Bent S and Ko R. Commonly used herbal medicines in the United States: A review. Am J Med. 2004; 116:478-485.
Badawya AM, Hassanean HA, Ibrahim AK, Habib ES, Ahmed SA. Review article on chemical constituents and biological activity of Thymelaea hirsuta. Rec. Pharm. Biomed. Sci. 2019; 3(2):28- 32.
Kawano M, Matsuyama K, Miyamae Y, Shinmoto H, Kchouk ME, Morio T, Shigemori H, Isoda H. Antimelanogenesis effect of Tunisian herb Thymelaea hirsuta extract on B16 murine melanoma cells. Exp Dermatol. 2007; 16(12):977-984.
Villareal MO, Han J, Yamada P, Shigemori H, Isoda H. Hirseins inhibit melanogenesis by regulating the gene expressions of Mitf and melanogenesis enzymes. Exp. Dermatol. 2010; 19(5):450-7.
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2005; 97:654–660.
Bnouham M, Merhfour FZ, Legssyer A, Mekhfi H, Maâllem S, Ziyyat A. Antihyperglycemic activity of Arbutus unedo, Ammoides pusilla, and Thymelaea hirsuta. Pharmazie. 2007; 62(8):630-632.
Miyamae Y, Villareal MO, Abdrabbah MB, Isoda H, Shigemori H. Hirseins A and B, daphnane diterpenoids from Thymelaea hirsuta that inhibit melanogenesis in B16 melanoma cells. J Nat Prod. 2009; 72:938‑41.
Frank CLU. 1992. Toxicologie, Données générales, procédures d’évaluation, organes cibles, évaluation du risque. Paris.
Bidhe RM and Ghosh S. Acute and subchronic (28 days) oral toxicity study in rats fed with novel surfactants. The AAPS Journal. 2004; 6:1-10.
Rahman MF Siddiqui MK and Jamil K. Effects of Vepacide (Azadirachta indica) on aspartate and alanine aminotransferase profiles in a subchronic study with rats. Human and Experimental Toxicology. 2001; 20:243–249.
Mdhluli M. 2003.Toxicological and antifertility investigations of oleanolic acid in male vervet monkeys (Chlorocebus aethiops).PhD Thesis.Discipline of Physiological Sciences, University of the Western Cape, Cape Town, South Africa. PathCare, 2009.Laboratory Manual. Parow, Cape Town, South Africa.
karmen A, Wroblewski F, Ladue JS. Transaminase activity in human blood. J Clin Invest. 1955; 34:126-131.
Kallai L, Hahn A, Roeder V, Županic V. Correlation between histological findings and serum transaminase values in chronic diseases of the liver. Acta Medica Scandinavica. 1964; 175:49-56.
Akhtar A, Deshmukh AA, Raut CG, Somkuwar AP and Bhagat SS. Prallethrin induced serum biochemical changes in Wistar rats. Pesticide Biochemistry and Physiology. 2012 ; 102:160-168.
Chiang J.. Liver Physiology: Metabolism and Detoxification. Pathobiology of Human Disease. 2014 ; 1770-1782.
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