In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts
The aim of this study is to evaluate in vitro antioxidant activities of Ruta montana L. extracts. This activity was evaluated by three methods : DPPH (2, 2'-diphenyl- 1- picrylhydrazy), bleaching of β-carotene and chelation of ferrous iron. Results showed that ethyl acetate extract (EAE) represents the highest amount of total polyphenols, tannins and flavonoids with 257,1 ± 0,703µg gallic acid equivalent/mg of extract, 251 ± 1.41 µg tannic acid equivalent /mg of extract,117,4 ± 3,451 µg quercetin equivalents/mg of extract, 139,5 ± 4,107 µg rutin equivalents/mg of extract, respectively. In the DPPH assay, ethyl acetate extract showed the higher scavenging capacity (IC50 = 0.044 ± 0.001 mg/ml) followed by methanol, aqueous and chloroform extract. Whereas, AqE showed the best chelating effect and the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene.
Keywords: Ruta montana L; polyphenols; antioxidant activity; free radical scavenging.
 Sisein E.A. Biochemistry of Free Radicals and Antioxidants. Sch. Acad. J. Biosci., 2014, 2(2), 110-118
 Beris H. Antioxidant effects a basis of drug selection. Drugs 1991; 42: 569-605.
 Rusmana D, Wahyudianingsih R, Elisabeth M, Maesaroh B, Widowati W. Antioxidant Activity of Phyllanthus niruri Extract, Rutin and Quercetin. Indones Biomed J., 2017, 9(2), 84-90.
 Herlina N, Riyanto S, Martono S and Abdul Rohman. Antioxidant Activities, Phenolic and Flavonoid Contents of Methanolic Extract of Stelechocarpus burahol Fruit and its Fractions. Dhaka Univ. J. Pharm. Sci., 2018, 17(2), 153-159.
 Kara Ali W, Ihoual S and Abidli N. Antioxidant and MDR reversal activity in resistant human ovarian cancer cells of methanolic extract from Ruta Montana located in the North of Algeria, Der Pharma Chemica., 2016, 8(12), 215-223.
 Sunday OO, Uguru OM. Peripheral and central antinociceptive activities of the crude methanolic extract and fractions of Momordicabalsaminalinn. Asian Journal of Pharmaceutical and Clinical Research., 2010, 0974-2441.
 Li HB, Cheng KW, Wong CC, Fan KW, Chen F, Jiang Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chemistry., 2007, 102, 771-776.
 Bahorun T, Gressier B, Trotin F, Brunete C, Dine T, Vasseur J, Gazin JC, Pinkas M, Luycky M, Gazin M. Oxygen species scavenging activity of phenolic extract from Hawthorn fresh plant organs and pharmaceutical preparation. Drug Research., 1996; 16.
 Bate-Smith EC. Haemanalysis of tannins, the concept of relativeastringency. Phytochemistry., 1973, 12, 907–12.
 Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research., 2000, 14, 323-328.
 Kartal N, Sokmen M, Tepe B, Daferera D, Polissiou M, Sokmen A. Investigation of the antioxidant properties of Ferula orientalis L. using a suitable extraction procedure. Food chemistry., 2007, 100, 584–589.
 Le K, Chiu F, Ng K. Identification and quantification of antioxidants in Fructus lycii. Food Chemistry., 2007, 105, 353-363.
 Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol., 2004, 26 (2), 211-219.
 Chouhan HS, Singh SK. Phytochemical analysis, antioxidant and anti-inflammatory activities of Phyllanthus simplex. J of Ethnopharmacology., 2011, 137, 1337-1344.
 Wangensteen H, Samuelsen AB, Maltrud KE. Antioxidant activity in extracts from coriander. Food Chem., 2004, 88, 293–297.
 Zielinski H, Kozlowska H. Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions. J Agric Food Chem., 2000, 48, 2008–2016 .
 Sun T, Ho CT. Antioxidant activities of buckwheat extracts. Food Chem., 2005, 90, 743–749.
 Kaur C, Kapoor HC. Anti-oxidant activity and total phenolic content of some Asian vegetables. Inter J Food SciTech., 2002, 37, 153–161.
 Kähkonen MP, Hopia AI, Heinonen M. Berry phenolics and their antioxidative activity. J. Agric. Food Chem., 2001, 49, 4076-4082.
 Vinson JA, Su XH, Zubik L, Bose P. Phenol antioxidant quantity and quality in foods: Fruits. J Agric Food Chem., 2001, 49, 5315–5321.
 Burda S, Oleszek W. Antioxidant and antiradical activities of flavonoids. Journal of Agricultural and Food Chemistry., 2001, 49, 2774 - 2779.
 Aruoma O I. Methodological considerations for characterizing potential antioxidantactions of bioactive components in plant foods. Mutation Research., 2002, 9 (20), 523-524.
 Huguet AI, Manez S, Alcaraz MJ. Superoxide scavenging properties of flavonoids in a non-enzymic system. Z. Naturforsch 1990, 45, 19-24.
 Fukumoto L R, Mazza G. Assessing antioxidant and prooxidant activities of phenolic compounds. Journal of Agricultural and Food Chemistry., 2000, 48, 3597 - 3604.
 Sharififar F, Nudeh G D, Mitrajaldini M. Major flavanoids with antioxidant activity from Teucrium polium L. Food Chem., 2009, 112, 885-888.
 Silva MM, Santos MR, Caroco G, Rocha R, Justiuo G, Mira L. Structure antioxidant activity relationships of flavonoids: A -Re-examination. Free radical Research., 2002, 36(11), 1219-1227.
 Furusawa M, Tanaka T, Ito T, Nishikawa A, amazaki NY, Nakaya K, Matsuura N, Tsuchiya H, Iinuma M. Antioxidant activity of hydroxyl flavonoids. J Health Sci., 2005, 51(3), 376-378.
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