COMPARATIVE STUDY OF Î’-CYCLODEXTRIN PRODUCTION FROM THE CGTASE PRODUCING NOVEL STRAINS- ITS CHARACTERIZATION AND HOST-GUEST COMPLEX FORMATION
The enzymatic synthesis of Î²-CD by CGTase produced from different strains of alkaliphilic bacterial culture which was isolated from the cultivated sugarcane fields and standard MTCC cultures using starch as substrate. An alkalophilic bacteria were grown for six days at static conditions at pH 10.5. The time course of CGTase activity was studied with the maximum activity observed on 6th day. The activity check and its confirmation were done by Dextrinising and Phenolphthalein assay. The soluble starch was best substrate to produce the cyclodextrin. Extraction of Î²-CD was done using complexing agents, these agents binds only to Î²-CD and forms complex, the complex thus formed was recovered. The obtained Î²-CD was made inclusion complex with guest molecule and was further characterized using UV absorption spectrophotometer, FT-IR and melting point.
Cyclodextrin glycosyltransferases, Cyclodextrins, Bacillus licheniformis.
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