RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN AND DIFLUPREDNATE IN THEIR COMBINED DOSAGE FORM
AbstractMoxifloxacin is synthetic fluoroquinolone antibiotic agent and Difluprednate is a topical corticosteroid indicated for the treatment of infammation and pain associated with ocular surgery. This combination of drugs will be used to treat optical infections. In RP-HPLC method, a mobile phase of Phosphate Buffer (pH 3.5) : Methanol (35:65 v/v)Â was used to resolve Moxifloxacin and Difluprednate from a mixture. The linearity range obtained for the HPLC method were 25 - 75 Î¼g/ml and 2.5 â€“ 7.5 Î¼g/ml with corresponding correlation coefficient of 0.998 and 0.997, for Moxifloxacin and Difluprednate respectively. Flow rate was set to 1 ml/min and detection was carried out at 244 nm. The method was found to be rapid, accurate and precise. This method was validated according to ICH guidlines.
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Patel M. RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN AND DIFLUPREDNATE IN THEIR COMBINED DOSAGE FORM. JDDT [Internet]. 14Jul.2014 [cited 22Feb.2024];4(4):60-5. Available from: https://jddtonline.info/index.php/jddt/article/view/913
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