Qualitative phytochemical screening and in vitro anti-inflammatory activities of Raphionacme brownii Scott-Elliot (Apocynaceae)
Abstract
Our study involved a qualitative phytochemical analysis and an evaluation of the anti-inflammatory activities of Raphionacme brownii tubers . To achieve these objectives, we employed several methods. Secondary metabolites were analyzed qualitatively. Anti-inflammatory activities were tested by inhibition of albumin denaturation, an antihemolytic assay, and inhibition of proteinase and lipoxygenase enzymes. Our results showed that the qualitative analysis revealed the presence of polyphenols, flavonoids, saponins, tannins, alkaloids, terpenoids, and sugars. The strongest capacity of the plant to inhibit albumin denaturation was observed in the aqueous extract (78.24 ± 0.17 µg/ml). The aqueous extract (3.16 ± 0.02 µg/ml) also exhibited rapid inhibition of proteinase. The aqueous extract (2.44 ± 0.016 µg/ml) inhibited lipoxygenase more rapidly than diclofenac. The capacity of R. brownii tubers to inhibit heat-induced hemolysis was higher in the methanol extract (3.35 ± 0.005 µg/ml). However, the different extracts showed rapid but weak anti-inflammatory activity compared to diclofenac. The richness in bioactive compounds and the anti-inflammatory activity could justify the traditional use of Raphionacme brownii against cardiovascular diseases.
Keywords: Raphionacme brownii , phytochemical screening, anti-inflammatory
Keywords:
Raphionacme brownii, phytochemical screening, anti-inflammatoryDOI
https://doi.org/10.22270/jddt.v16i6.7791References
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