Xanthine Oxidase Inhibition and Anti-Lung Cancer Effects of the Lonicera japonica Thunb–Paris polyphylla var. chinensis Herbal Pair
Abstract
In Vietnamese folk medicine Lonicera japonica Thunb. flowers (LTF) had recognized significantly attentions for gout treatment therapies, and LTF is frequently combined with the rhizomes of Paris polyphylla var. chinensis (PPR) for lung cancer remedy. This study evaluated in vitro therapeutic potential of this herbal pair. Their chemical structures were explicated by extensive spectroscopic analyses, covering MS and NMR spectra. The xanthine oxidase preventive effects of the isolated compounds and their extract mixtures, as well as their effects on A549 cell repressive capacity, were researched. Ferulic acid was identified for the first time from LTF together with chlorogenic acid, while quercetin and β-sitosterol-3-O-β-D-glucopyranoside were isolated from PPR. Mixture of PPR-MeOH and E50B (at ratio 1:1) restricted growth of A549 cancer cells with IC50 of 4.11±0.16 µg/mL. The LTF–PPR mixture also activated the apoptotic factors comprising caspase-3, caspase-9, and p53. The findings demonstrated for the first time the XO inhibitory capactity of PPR-MeOH and E50B are 42.12 ± 0.34 and 33.89±1.36, respectively. The present results recommended that applying LTF and PPR in gout and lung cancer treatment needs to insightly more research in the future.
Keywords: Lonicera Japonica Thunb, Paris polyphylla, enzyme xanthine oxidase, anti-lung cancer.
Keywords:
Lonicera Japonica Thunb, Paris polyphylla, enzyme xanthine oxidase, anti-lung cancerDOI
https://doi.org/10.22270/jddt.v16i5.7759References
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