Implementation and Evaluation of 24-Hours Matured Cold Process Anti-Acne Herbal Soap using Ocimum sanctum and Mentha piperita Peppermint Essential Oil

Authors

  • M Kannahi PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India. https://orcid.org/0009-0003-6619-3888
  • G Iswarya PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.
  • S Kamali PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.
  • S Madhumidha PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.
  • B Manimozhi PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.
  • N Najibah Banu PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.
  • R Santhoshini PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

Abstract

The present study focuses on the preparation and evaluation of a cold process anti-acne herbal soap matured within 24 hours using natural ingredients such as tulsi (Ocimum sanctum) and peppermint essential oil (Mentha piperita). The soap was formulated using vegetable oils including coconut oil, olive oil, and castor oil, along with additives such as sodium lactate, sugar, and salt to reduce curing time. The traditional cold process method usually requires 4–6 weeks of curing; however, this study successfully reduced the maturation time to 24 hours. The prepared soap was evaluated for physicochemical properties such as pH, hardness, foam stability, and antimicrobial activity against acne-causing bacteria like Cutibacterium acnes and Staphylococcus epidermidis. The results indicated good performance with effective antimicrobial activity, stable foam formation, and an acceptable pH for skin application. The study concludes that the developed herbal soap is a safe, efficient, and natural alternative to synthetic anti-acne soaps.

Keywords: Herbal soap, anti-acne, Ocimum sanctum, peppermint essential oil, cold process, antimicrobial activity.

Keywords:

Herbal soap, anti-acne, Ocimum sanctum, peppermint essential oil, cold process, antimicrobial activity

DOI

https://doi.org/10.22270/jddt.v16i6.7757

Author Biographies

M Kannahi, PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

G Iswarya , PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

S Kamali , PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

S Madhumidha , PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

B Manimozhi , PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

N Najibah Banu , PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

R Santhoshini , PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India.

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Published

2026-06-15
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How to Cite

1.
Kannahi M, Iswarya G, Kamali S, Madhumidha S, Manimozhi B, Najibah Banu N, et al. Implementation and Evaluation of 24-Hours Matured Cold Process Anti-Acne Herbal Soap using Ocimum sanctum and Mentha piperita Peppermint Essential Oil. J. Drug Delivery Ther. [Internet]. 2026 Jun. 15 [cited 2026 Jun. 15];16(6):73-9. Available from: https://jddtonline.info/index.php/jddt/article/view/7757

Issue

Vol. 16 No. 6 (2026): Volume 16, Issue 6, June 2026

Section

Research

Copyright (c) 2026 M Kannahi, G Iswarya , S Kamali , S Madhumidha , B Manimozhi , N Najibah Banu , R Santhoshini

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How to Cite

1.
Kannahi M, Iswarya G, Kamali S, Madhumidha S, Manimozhi B, Najibah Banu N, et al. Implementation and Evaluation of 24-Hours Matured Cold Process Anti-Acne Herbal Soap using Ocimum sanctum and Mentha piperita Peppermint Essential Oil. J. Drug Delivery Ther. [Internet]. 2026 Jun. 15 [cited 2026 Jun. 15];16(6):73-9. Available from: https://jddtonline.info/index.php/jddt/article/view/7757
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