Role of Omega-3 Polyunsaturated Fatty Acids in Human Health: Chemistry, Mechanisms, and Clinical Evidence
Abstract
Omega-3 polyunsaturated fatty acids (PUFAs) are a structurally distinct class of lipids characterized by a cis double bond at the third carbon from the methyl (ω) terminus. Major nutritionally relevant forms include α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA). This review synthesizes recent advances in the chemistry, metabolism, and clinical significance of omega-3 PUFAs. Emphasis is placed on their structural features, susceptibility to oxidation, conversion into specialised pro-resolving mediators, and incorporation into biological membranes. These chemical properties are linked to their mechanistic roles in cardiovascular, metabolic, neurological, inflammatory, and developmental health. Emerging aspects such as sustainable omega-3 sources, bioavailability, and the omega-3 index as a biomarker are also discussed. Current clinical evidence highlights that omega-3 PUFAs exert their effects through membrane modulation, receptor signalling, and lipid-mediator pathways. Future research should integrate lipidomics and personalised nutrition approaches to optimise therapeutic applications.
Keywords: Omega-3 fatty acids; EPA; DHA; PUFA; lipid metabolism; clinical health
Keywords:
Omega-3 fatty acids, EPA , DHA, PUFA, Lipid metabolism, clinical healthDOI
https://doi.org/10.22270/jddt.v16i5.7718References
1. Shahidi F, Ambigaipalan P. Omega-3 polyunsaturated fatty acids and their health benefits. Annual review of food science and technology. 2018;9(1):345-81. https://doi.org/10.1146/annurev-food-111317-095850 PMid:29350557
2. Krupa KN, Fritz K, Parmar M. Omega-3 fatty acids. StatPearls [Internet]: StatPearls Publishing; 2024.
3. Saini RK, Prasad P, Sreedhar RV, Akhilender Naidu K, Shang X, Keum Y-S. Omega− 3 polyunsaturated fatty acids (PUFAs): Emerging plant and microbial sources, oxidative stability, bioavailability, and health benefits-A review. Antioxidants. 2021;10(10):1627. https://doi.org/10.3390/antiox10101627 PMid:34679761 PMCid:PMC8533147
4. Dicklin MR, Anthony JC, Winters BL, Maki KC. ω-3 polyunsaturated fatty acid status testing in humans: a narrative review of commercially available options. The Journal of Nutrition. 2024;154(5):1487-504. https://doi.org/10.1016/j.tjnut.2024.03.015 PMid:38522783
5. Harris WS. Recent studies confirm the utility of the omega-3 index. Current Opinion in Clinical Nutrition & Metabolic Care. 2025;28(2):91-5. https://doi.org/10.1097/MCO.0000000000001078 PMid:39514368 PMCid:PMC11809719
6. Gutierres D, Pacheco R, Reis CP. The Role of Omega-3 and Omega-6 Polyunsaturated Fatty Acid Supplementation in Human Health. Foods. 2025;14(19):3299. PMCid:PMC12524211 https://doi.org/10.3390/foods14193299 PMid:41097470
7. Wen J, Satyanarayanan SK, Li A, Yan L, Zhao Z, Yuan Q, et al. Unraveling the impact of Omega-3 polyunsaturated fatty acids on blood-brain barrier (BBB) integrity and glymphatic function. Brain, Behavior, and Immunity. 2024;115:335-55. https://doi.org/10.1016/j.bbi.2023.10.018 PMid:37914102
8. Patil D. Role of antioxidants in stability of edible oil. Trends Post-Harvest Technol. 2013;1:68-73.
9. Ghazal RM, Naffaa MM. Omega-3 fatty acids and fetal brain development: Implications for maternal nutrition, mechanisms of cognitive function, and pediatric depression. Exploration of Neuroprotective Therapy. 2025;5:1004107. https://doi.org/10.37349/ent.2025.1004107
10. Calder PC. Eicosapentaenoic and docosahexaenoic acid derived specialised pro-resolving mediators: Concentrations in humans and the effects of age, sex, disease and increased omega-3 fatty acid intake. Biochimie. 2020;178:105-23. https://doi.org/10.1016/j.biochi.2020.08.015 PMid:32860894
11. Chiang N, Serhan CN. Specialized pro-resolving mediator network: an update on production and actions. Essays in Biochemistry. 2020;64(3):443-62. https://doi.org/10.1042/EBC20200018 PMid:32885825 PMCid:PMC7682745
12. Rasquel-Oliveira FS, Silva MDVd, Martelossi-Cebinelli G, Fattori V, Casagrande R, Verri Jr WA. Specialized pro-resolving lipid mediators: Endogenous roles and pharmacological activities in infections. Molecules. 2023;28(13):5032. PMid:37446699 PMCid:PMC10343836 https://doi.org/10.3390/molecules28135032
13. Dalli J, Gomez EA, Jouvene CC. Utility of the specialized pro-resolving mediators as diagnostic and prognostic biomarkers in disease. Biomolecules. 2022;12(3):353. PMid:35327544 PMCid:PMC8945731 https://doi.org/10.3390/biom12030353
14. Alijani S, Hahn A, Harris WS, Schuchardt JP. Bioavailability of EPA and DHA in humans-A comprehensive review. Progress in Lipid Research. 2025;97:101318. https://doi.org/10.1016/j.plipres.2024.101318 PMid:39736417
15. Mohanty BP, Ganguly S, Mahanty A, Sankar T, Anandan R, Chakraborty K, et al. DHA and EPA content and fatty acid profile of 39 food fishes from India. BioMed research international. 2016;2016(1):4027437. https://doi.org/10.1155/2016/4027437 PMid:27579313 PMCid:PMC4989070
16. Patil D, Nag A. Production of PUFA concentrates from poultry and fish processing waste. Journal of the American Oil Chemists' Society. 2011;88(4):589-93. https://doi.org/10.1007/s11746-010-1689-4
17. Patil D. Recent trends in production of polyunsaturated fatty acids (PUFA) concentrates. J Food Res Technol. 2014;2:15-23.
18. Rizzo G, Baroni L, Lombardo M. Promising sources of plant-derived polyunsaturated fatty acids: A narrative review. International Journal of Environmental Research and Public Health. 2023;20(3):1683. https://doi.org/10.3390/ijerph20031683 PMid:36767052 PMCid:PMC9914036
19. Bailey E, Wojcik J, Rahn M, Roos F, Spooren A, Koshibu K. Comparative Bioavailability of DHA and EPA from Microalgal and Fish Oil in Adults. International Journal of Molecular Sciences. 2025;26(19):9343. https://doi.org/10.3390/ijms26199343 PMid:41096614 PMCid:PMC12524788
20. Qin J, Kurt E, LBassi T, Sa L, Xie D. Biotechnological production of omega-3 fatty acids: Current status and future perspectives. Frontiers in microbiology. 2023;14:1280296. PMid:38029217 PMCid:PMC10662050 https://doi.org/10.3389/fmicb.2023.1280296
21. Ghorbanzade T, Jafari SM, Akhavan S, Hadavi R. Nano-encapsulation of fish oil in nano-liposomes and its application in fortification of yogurt. Food chemistry. 2017;216:146-52. https://doi.org/10.1016/j.foodchem.2016.08.022 PMid:27596403
22. Swetha N, Mathanghi S. Towards sustainable omega-3 fatty acids production-A comprehensive review on extraction methods, oxidative stability and bio-availability enhancement. Food Chemistry Advances. 2024;4:100603. https://doi.org/10.1016/j.focha.2023.100603
23. Dempsey M, Rockwell MS, Wentz LM. The influence of dietary and supplemental omega-3 fatty acids on the omega-3 index: A scoping review. Frontiers in Nutrition. [Systematic Review]. 2023 2023-January-19;Volume 10 - 2023. PMid:36742439 PMCid:PMC9892774 https://doi.org/10.3389/fnut.2023.1072653
24. Nevins JE, Donovan SM, Snetselaar L, Dewey KG, Novotny R, Stang J, et al. Omega-3 fatty acid dietary supplements consumed during pregnancy and lactation and child neurodevelopment: a systematic review. The Journal of Nutrition. 2021;151(11):3483-94. https://doi.org/10.1093/jn/nxab238 PMid:34383914 PMCid:PMC8764572
25. Xu R, Molenaar AJ, Chen Z, Yuan Y. Mode and mechanism of action of Omega-3 and Omega-6 unsaturated fatty acids in chronic diseases. Nutrients. 2025;17(9):1540. PMid:40362847 PMCid:PMC12073370 https://doi.org/10.3390/nu17091540
26. Serefko A, Jach ME, Pietraszuk M, Świąder M, Świąder K, Szopa A. Omega-3 polyunsaturated fatty acids in depression. International Journal of Molecular Sciences. 2024;25(16):8675. https://doi.org/10.3390/ijms25168675 PMid:39201362 PMCid:PMC11354246
27. Xiao Y, Zhang Q, Liao X, Elbelt U, Weylandt KH. The effects of omega-3 fatty acids in type 2 diabetes: A systematic review and meta-analysis. Prostaglandins, Leukotrienes and Essential Fatty Acids. 2022;182:102456. https://doi.org/10.1016/j.plefa.2022.102456 PMid:35717726
28. Norouziasl R, Zeraattalab-Motlagh S, Jayedi A, Shab-Bidar S. Efficacy and safety of n-3 fatty acids supplementation on depression: a systematic review and dose-response meta-analysis of randomised controlled trials. British Journal of Nutrition. 2024;131(4):658-71. https://doi.org/10.1017/S0007114523002052 PMid:37726108
29. Barros MI, Brandão T, Irving SC, Alves P, Gomes F, Correia M. Omega-3 Polyunsaturated Fatty Acids and Cognitive Decline in Adults with Non-Dementia or Mild Cognitive Impairment: An Overview of Systematic Reviews. Nutrients. 2025;17(18):3002. https://doi.org/10.3390/nu17183002 PMid:41010527 PMCid:PMC12472900
30. Lee WM, Ryu SI, Lim M-N, Han JW, Bae JB, Kim TH, et al. Association Between Omega-3 Supplement Use and Cognitive Function in Korean Older Adults: An 8-Year Longitudinal Cohort Study. The Journal of nutrition, health and aging. 2026 2026/01/01/;30(1):100739. https://doi.org/10.1016/j.jnha.2025.100739 PMid:41308405 PMCid:PMC12702202
31. Bazinet RP, Layé S. Polyunsaturated fatty acids and their metabolites in brain function and disease. Nature reviews neuroscience. 2014;15(12):771-85. https://doi.org/10.1038/nrn3820 PMid:25387473
32. Bayram SŞ, Kızıltan G. The Role of Omega- 3 Polyunsaturated Fatty Acids in Diabetes Mellitus Management: A Narrative Review. Current Nutrition Reports. 2024 2024/09/01;13(3):527-51. https://doi.org/10.1007/s13668-024-00561-9 PMid:39031306 PMCid:PMC11327211
33. Ali Z, Al-Ghouti MA, Abou-Saleh H, Rahman MM. Unraveling the Omega-3 Puzzle: Navigating Challenges and Innovations for Bone Health and Healthy Aging. Marine Drugs. 2024;22(10):446. https://doi.org/10.3390/md22100446 PMid:39452854 PMCid:PMC11509197
34. Adarme-Vega TC, Lim DK, Timmins M, Vernen F, Li Y, Schenk PM. Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production. Microbial cell factories. 2012;11(1):96. https://doi.org/10.1186/1475-2859-11-96 PMid:22830315 PMCid:PMC3465194
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