A Physicochemical and Chromatographic Fingerprint Study on Tilvak Ghrita: A Polyherbal Formulation
Abstract
Background: Tilvak Ghrita is a classical Ayurvedic polyherbal formulation described in the Ayurvedic literature Ashtang Hridaya indicated in Vata Vyadhi Chikitsa. In view of its therapeutic utility and increasing clinical relevance, establishment of physiochemical analysis is essential.
Objective: To evaluate the physicochemical parameters and develop a chromatographic fingerprint of Tilvak Ghrita for quality standardization, as per Ayurvedic Pharmacopoeia of India (API) guidelines.
Materials and Methods: Tilvak Ghrita was prepared in accordance with classical references following Good Manufacturing Practices (GMP). Tilvak Ghrita formulations was evaluated for the physio-chemical parameters like rancidity, moisture, iodine value, refractive index, saponification value, specific gravity etc and finger printing by thin layer chromatography (TLC).
Results: Physicochemical analysis showed no rancidity, 226.90 saponification value, 37.87 iodine value, 1.4611 refractive index, 1.55 acid value and 0.16% moisture content. TLC analysis demonstrated distinct The Rf values for these spots were observed at 0.25, 0.31 and 0.80 at 365nm, indicating the presence of multiple phyto-constituents.
Conclusion: The physicochemical parameters may serve as reference quality control benchmarks for ensuring the identity, purity and consistency of the formulation, in accordance with API requirements. The chromatographic fingerprinting showed multiple phyto-constituents which validate this herbal formulation in today perspective.
Keywords: Tilvak Ghrita, Vata Vyadhi, Organoleptic Properties, Physicochemical Parameters, Thin-Layer Chromatography.
Keywords:
Tilvak Ghrita, Vata Vyadhi, Organoleptic Properties, Physicochemical Parameters, Thin-Layer ChromatographyDOI
https://doi.org/10.22270/jddt.v16i5.7690References
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