Impact of ZnO nanoparticles on growth and biochemical characteristics of Macrotyloma uniflorum (LAM.) Verdcourt
Abstract
The present work reports the green synthesis of zinc oxide nanoparticles (ZnO NPs) using green tea leaves and its effect on germination, morphology and some biochemical attributes of Macrotyloma uniflorum (kollu or horse gram). The phytosynthesized ZnO NPs were characterized by UV-VIS, FTIR, Zeta sizer and SEM. After surface sterilization, the overnight soaked seeds of horse gram were treated with different concentrations of green synthesized ZnO NPs (10 – 50 mg/L) and allowed to germinate at room temperature with regular spraying of respective nano-formulations for 15 days. On fifth day of growth, germination percentage of seeds was determined. Shoot and root lengths were measured on 15th day of seedling growth. At the end of the experiment, the whole plants were harvested and used for estimation of some primary metabolites and secondary metabolites using the standard procedures. The data obtained in the experiment were statistically analysed. UV-VIS and FTIR analyses confirm that the polyphenol contents of green tea leaves are involved in the biosynthesis of ZnO NPs. Stronger stability and semi-spherical morphology of green synthesized ZnO NPs were confirmed by zeta potential value and SEM, respectively. Higher levels of germination percentage, shoot and root lengths, primary metabolites (total chlorophyll, protein, carbohydrates) and secondary metabolites (total phenols, flavonoids, tannins and terpenoids) were observed in 10 and 20 mg/L ZnO NPs treated plants compared to control. Thus, the results demonstrate that the application of ZnO NPs at a lesser concentration may increase seed germination, morphological and biochemical attributes of horse gram seedlings.
Keywords: Macrotyloma uniflorum, horse gram, green synthesis, ZnO NPs, Zeta Sizer, biochemical attributes
Keywords:
Macrotyloma uniflorum, biochemical attributes, green synthesis, ZnO NPsDOI
https://doi.org/10.22270/jddt.v15i12.7400References
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