Antioxidant Potential of Arogyavardhini Vati Extract by DPPH Assay
Abstract
Arogyavardhini Vati, an Ayurvedic medicine, was commonly used during the COVID-19 pandemic for its immunomodulatory and therapeutic properties. The DPPH radical scavenging assay in this study revealed its antioxidant potential. This Ayurvedic medicine demonstrated considerable antioxidant capability with 78.04% DPPH scavenging activity. These findings support its traditional use and suggest the need for further investigation.
Keywords: Arogyavardhini Vati, DPPH assay, antioxidant activity, ethanol extract, COVID-19, Soxhlet’s extraction, etc.
Keywords:
Arogyavardhini Vati, DPPH assay, antioxidant activity, COVID-19DOI
https://doi.org/10.22270/jddt.v15i7.7254References
1. Kumar S, Kumar D, Saroha K, Singh N, Vashishta B. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract. Acta Pharmaceutica. 2008 Jun 1;58(2):215-20. https://doi.org/10.2478/v10007-008-0008-1
2. Sulaiman CT, Balachandran I. Total phenolics and total flavonoids in selected Indian medicinal plants. Indian journal of pharmaceutical sciences. 2012 May;74(3):258. doi: https://doi.org/10.4103/0250-474X.106069
3. Kumar G, Srivastava A, Sharma SK, Gupta YK. The hypolipidemic activity of Ayurvedic medicine, Arogyavardhini iati in Triton WR-1339-induced hyperlipidemic rats: a comparison with fenofibrate. Journal of Ayurveda and integrative medicine. 2013 Jul;4(3):165. doi: https://doi.org/10.4103/0975-9476.118707
4. Sarashetti RS, Simpi CC, Sandeep NM, Kanthi VG. Screening of free radical scavenging activity of Arogyavardhini Vati. 2013 Sep 25;4(4):555-559. http://www.ijrap.net/admin/php/uploads/1062_pdf.pdf
5. Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, Gargiulo G, Testa G, Cacciatore F, Bonaduce D, Abete P. Oxidative stress, aging, and diseases. Clinical interventions in aging. 2018 Apr 26:757-72. https://doi.org/10.2147/CIA.S158513
6. Betteridge DJ. What is oxidative stress? Metabolism. 2000 Feb 1;49(2):3-8. https://doi.org/10.1016/S0026-0495(00)80077-3
7. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. The international journal of biochemistry & cell biology. 2007 Jan 1;39(1):44-84. https://doi.org/10.1016/j.biocel.2006.07.001
8. Delgado-Roche L, Mesta F. Oxidative stress as key player in severe acute respiratory syndrome coronavirus (SARS-CoV) infection. Archives of medical research. 2020 Jul 1;51(5):384-7. https://doi.org/10.1016/j.arcmed.2020.04.019
9. Chambyal K, Sharma S, Sharma S. Ayurveda prophylaxis and intervention for COVID-19 Pandemic. Journal of Ayurveda. 2020 Oct 1;14(4):22-7. https://doi.org/10.4103/joa.joa_140_20
10. Tawalare K. 3D Approach of Ayurveda to Combat COVID-19: Prevention, Intervention, and Rehabilitation (PIR). AYUSH: International Research Journal of Ayurveda Teachers Association. 2022 Oct 12;1(1).
11. Shekade PP, Pundge RS, Chavan SS, Dhabe AS. Phytochemical profile of Alysicarpus hamosus Edgew. as derived by HRLC-MS technique. Bioinfolet. 2023;20(2a):262–265.
12. Shastri AD (Ed.). Bhaishajya Ratnavali (B. B. R.), Part I. 18th ed. Varanasi: Chaukhambha Sanskrit Bhawan; 2012. p. 448.
13. Rajkumar V, Guha G, Kumar RA. Antioxidant and anti-neoplastic activities of Picrorhiza kurroa extracts. Food and Chemical Toxicology. 2011 Feb 1;49(2):363-9. https://doi.org/10.1016/j.fct.2010.11.009
14. Mirunalini S, Krishnaveni M. Therapeutic potential of Phyllanthus emblica (amla): the ayurvedic wonder. Journal of basic and clinical physiology and pharmacology. 2010 Feb;21(1):93-105. https://doi.org/10.1515/JBCPP.2010.21.1.93
15. Saleem A, Husheem M, Härkönen P, Pihlaja K. Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula Retz. fruit. J Ethnopharmacol. 2002 Aug;81(3):327–36. https://doi.org/10.1016/S0378-8741(02)00099-5
16. Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK, Mohan H. Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry. 2003 May 1;63(1):97-104. https://doi.org/10.1016/S0031-9422(02)00754-9Get rights and content
17. Satyavati GV. Gum guggul (Commiphora mukul)-the success story of an ancient insight leading to a modern discovery. 1988 Apr; 87:327-35.
18. Iyengar MA, Pendse GS. Plumbago zeylanica L. (Chitrak) a gastrointestinal flora normaliser. Planta Medica. 1966 Aug;14(03):337-351. https://doi.org/10.1055/s-0028-1100060
19. Sheeja E, Joshi SB, Jain DC. Bioassay-guided isolation of anti-inflammatory and antinociceptive compound from Plumbago zeylanica leaf. Pharmaceutical biology. 2010 Apr 1;48(4):381-7. https://doi.org/10.3109/13880200903156424
20. Subapriya R, Nagini S. Medicinal properties of neem leaves: a review. Current Medicinal Chemistry-Anti-Cancer Agents. 2005 Mar 1;5(2):149-56. https://doi.org/10.2174/1568011053174828
21. Biswas K, Chattopadhyay I, Banerjee RK, Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Current science. 2002 Jun 10:1336-45. http://www.jstor.org/stable/24106000. Accessed 21 June 2025.
22. Udeinya IJ, Mbah AU, Chijioke CP, Shu EN. An antimalarial extract from neem leaves is antiretroviral. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2004 Jul 1;98(7):435-7. https://doi.org/10.1016/j.trstmh.2003.10.016
23. Ghosal S, Lal J, Singh SK, Goel RK, Jaiswal AK, Bhattacharya SK. The need for formulation of Shilajit by its isolated active constituents. Phytotherapy research. 1991 Oct;5(5):211-6. https://doi.org/10.1002/ptr.2650050505
24. Tripathi YB, Singh VP. Role of Tamra bhasma, an Ayurvedic preparation, in the management of lipid peroxidation in liver of albino rats. Indian journal of experimental biology. 1996 Jan 1;34(1):66-70. PMID: 8698411.
25. Balkrishna A, Solleti SK, Singh H, Singh R, Sharma N, Varshney A. Biotite-Calx based traditional Indian medicine sahastraputi-abhrak-bhasma prophylactically mitigates allergic airway inflammation in a mouse model of asthma by amending cytokine responses. Journal of Inflammation Research. 2021 Sep 17:4743-60. https://doi.org/10.2147/JIR.S313955
26. Joshi N, Dash MK, Dwivedi L, Khilnani G. Effect of Navayasa Lauha, an ayurvedic herbomineral formulation on experimentally-induced iron deficiency anemia. Ancient Science of Life. 2018 Apr 1;37(4):180-7. https://doi.org/10.4103/asl.ASL_5_19
27. Bhagwanrao SS. Antimicrobial activity of Shuddha Gandhaka synthesized by traditional Ayurvedic methods. Int J Ayu Med Res. 2015 July;3(7):2213–7.
28. Satyam B, Pati RK. Traditional aspects of Kajjali: processing and therapeutic insights. J Ayurveda Integr Med Sci. 2024;9(8):26–34.
29. Harborne AJ. Phytochemical methods a guide to modern techniques of plant analysis. springer science & business media; 1998 Apr 30.
30. Brand-Williams W, Cuvelier ME, Berset CL. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology. 1995 Jan 1;28(1):25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
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