Therapeutic importance of hydrolic fraction of Celtis integrifolia roots for prevention of brain oxidative stress, increase of acetyl cholinesterase activity and tissues damage in monosodium glutamate-induced neurodegenerative-like diseases in mice
Abstract
Background: Celtis integrifolia is a common plant with its roots traditionally used to alleviate nervous problems. The current study aimed to assess the potential effects on cholinergic activity and oxidative status in the brain, as well as its impact in preventing neurological damage in neurodegenerative diseases.
Methodology: 35 mice were randomly distributed in seven groups (N=5). They were exposed to the monosodium glutamate (MSG) (2 g/kg/day) and given the hydrolic fraction at different doses for 21 days. The day after, CAT, SOD and GSH, NO and MDA concentrations in brain as well as the AchE activity were measured using colorimetric methods. The histoarchitectures of brain regions were also examined.
Results: Compared to non-treated mice exposed to MSG (Negative), the findings of this study revealed that plant extract significantly increased the antioxidant enzymes activity (CAT, SOD and GSH) and significantly decreased the NO and MDA concentrations in brain mice exposed to MSG. Furthermore, the extract significantly increased levels of AChE activity compared to the negative control. Moreover, hydrolic fraction of C. integrifolia significantly prevented necrosis in the Ca1 and Ca3 regions hippocampal, cortex, amygdala and gyrus as well as loss of hilum of dentate gyrus in mice exposed to MSG.
Conclusion: In conclusion, this study demonstrates that the hydrophilic fraction of Celtis integrifolia root has a neuroprotective effect in MSG-induced neurodegenerative-like diseases.
Keywords: Celtis integrifolia; antioxidant; Brain histoarchitecture.
Keywords:
Celtis integrifolia; antioxidant; Brain histoarchitecture. , Celtis integrifolia, antioxidant, Brain histoarchitectureDOI
https://doi.org/10.22270/jddt.v15i7.7243References
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