A Comprehensive Review on Nano-Scaffolds in Regenerative Medicine: Types, Preparation Methods and Applications
Abstract
Nano-scaffolds are a major advancement in biomedical engineering, providing essential support for tissue repair, drug delivery, and regenerative medicine. These tiny structures have a high surface area, making them suitable for promoting cell growth and interaction with biological systems. They are created using methods like electrospinning, 3D printing, solvent casting, and biomimetic techniques, which allow for controlled strength, porosity, and biodegradability. Nano-scaffolds are extensively utilized in medicine to improve drug delivery by providing accurate and sustained release of therapeutic substances. In wound healing, they help tissue repair while reducing infections and inflammation. Bone regeneration benefits from scaffolds containing bioactive materials like hydroxyapatite, which support new bone formation. Additionally, conductive Nano-Scaffolds assist in nerve repair by guiding nerve growth and maintaining synaptic connections. Their potential in gene therapy is also significant, as they allow for controlled genetic modifications. Despite their many benefits, challenges such as large-scale production, immune response, and long-term stability still exist. Ongoing research aims to improve scaffold durability, incorporate smart biomaterials, and refine manufacturing techniques. With continuous advancements, Nano-Scaffolds hold great potential in revolutionizing regenerative medicine and personalized healthcare. This review covers the history of nano-scaffolds, types, preparation methods, and their uses in drug delivery, wound healing, bone regeneration, nerve repair, and gene therapy. It also highlights recent research and innovations, showing their future potential in medicine.
Keywords: Nano-scaffolds, Nanofibers, Hydrogels, Nanoparticles, Electrospinning
Keywords:
Nanoscaffolds, Nanofibers, Hydrogels, Nanoparticles, ElectrospinningDOI
https://doi.org/10.22270/jddt.v15i4.7083References
1. Robert Langer and Joseph P. Vacanti, Tissue Engineering, SCIENCE,1998; 260:920-926. https://doi.org/10.1126/science.8493529 PMid:8493529
2. Vacanti, Joseph P., and Robert Langer, Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation, The lancet 354 1999: S32-S34. https://doi.org/10.1016/S0140-6736(99)90247-7 PMid:10437854
3. Li, Wan‐Ju, Cato T. Laurencin, Edward J. Caterson, Rocky S. Tuan, and Frank K. Ko, Electrospun nanofibrous structure: a novel scaffold for tissue engineering, Journal of Biomedical Materials Research,An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials 2002;60(4):613-621. https://doi.org/10.1002/jbm.10167 PMid:11948520
4. Lahiri, Debrupa, Sanat Ghosh, and Arvind Agarwal, Carbon nanotube reinforced hydroxyapatite composite for orthopedic application: a review, Materials Science and Engineering: 2012;32(7):1727-1758. https://doi.org/10.1016/j.msec.2012.05.010 PMid:34062652
5. Murphy, Sean V., and Anthony Atala, 3D bioprinting of tissues and organs,Nature biotechnology 2014; 32(8):773-785. https://doi.org/10.1038/nbt.2958 PMid:25093879
6. Rani, Varsha, Rinki Verma, Krishan Kumar, and Ruchi Chawla,Role of pro-inflammatory cytokines in Alzheimer's disease and neuroprotective effects of pegylated self-assembled nanoscaffolds, Current research in pharmacology and drug discovery 2023;4:100149. https://doi.org/10.1016/j.crphar.2022.100149 PMid:36593925 PMCid:PMC9804106
7. Chen, X., Wang, Y., Zhou, G., Hu, X., Han, S. and Gao, J.,The combination of nanoscaffolds and stem cell transplantation: Paving a promising road for spinal cord injury regeneration. Biomedicine & Pharmacotherapy, 2021;143:112233. https://doi.org/10.1016/j.biopha.2021.112233 PMid:34649357
8. Li Y, Liu W, Wang Y, Lu S. Cellulose Based Nano-Scaffolds for Targeted Cancer Therapies: Current Status and Future Perspective. International Journal of Nanomedicine. 202;199-213 https://doi.org/10.2147/IJN.S500261 PMid:39802388 PMCid:PMC11721505
9. Filippi, M., Born, G., Chaaban, M. and Scherberich, A Natural polymeric scaffold in bone regeneration. Frontiers in bioengineering and biotechnology, 2020;8:474. https://doi.org/10.3389/fbioe.2020.00474 PMid:32509754 PMCid:PMC7253672
10. Kumar, A. and Jacob, A., Techniques in scaffold fabrication process for tissue engineering applications: A review. Journal of Applied Biology & Biotechnology 2022;10(3):163-176. https://doi.org/10.7324/JABB.2022.100321
11. Perez-Puyana, V., Jiménez-Rosado, M., Romero, A. and Guerrero, A., Polymer-based scaffolds for soft-tissue engineering. Polymers, Advanced Polymeric Biomaterials for Tissue Engineering, 2020;12(7):1566. https://doi.org/10.3390/polym12071566 PMid:32679750 PMCid:PMC7408565
12. Chocholata P, Kulda V, Babuska V. Fabrication of scaffolds for bone-tissue regeneration. Materials (Basel) 2019;12:568. https://doi.org/10.3390/ma12040568 PMid:30769821 PMCid:PMC6416573
13. Wang, Y., Chen, Y., Hou, Q., Ju, M. and Li, W., Coupling plasmonic and cocatalyst nanoparticles on N-TiO2 for visible-light-driven catalytic organic synthesis. Nanomaterials, 2019;9(3):391. https://doi.org/10.3390/nano9030391 PMid:30866493 PMCid:PMC6473962
14. Scott, T.G., Blackburn, G., Ashley, M., Bayer, I.S., Ghosh, A., Biris, A.S. and Biswas, A., Advances in bionanomaterials for bone tissue engineering. Journal of nanoscience and nanotechnology, 2013;13(1):1-22. https://doi.org/10.1166/jnn.2013.6733 PMid:23646693
15. Hamimed S, Jabberi M, Chatti A. Nanotechnology in drug and gene delivery. Naunyn-schmiedeberg's Archives of Pharmacology. 2022 Jul;395(7):769-787. https://doi.org/10.1007/s00210-022-02245-z PMid:35505234 PMCid:PMC9064725
16. Kumar P, Pandey SN, Ahmad F, Verma A, Sharma H, Ashique S, Bhattacharyya SP, Bhattacharyya I, Kumar S, Mishra N, Garg A. Carbon nanotubes: a targeted drug delivery against cancer cell. Current Nanoscience. 2024 Nov 1;20(6):769-800. https://doi.org/10.2174/0115734137271865231105070727
17. Alberti, T.B., Coelho, D.S., De Prá, M., Maraschin, M. and Veleirinho, B, Electrospun PVA nanoscaffolds associated with propolis nanoparticles with wound healing activity, Journal of Materials Science, 2020;55(23):9712-9727. https://doi.org/10.1007/s10853-020-04502-z
18. Farjadian, F., Ghasemi, A., Gohari, O., Roointan, A., Karimi, M. and Hamblin, M.R. Nano pharmaceuticals and nanomedicines currently on the market: challenges and opportunities. Nanomedicine, 2019;14(1):93-126. https://doi.org/10.2217/nnm-2018-0120 PMid:30451076 PMCid:PMC6391637
19. Sainz, V., Conniot, J., Matos, A.I., Peres, C., Zupanǒiǒ, E., Moura, L., Silva, L.C., Florindo, H.F. and Gaspar, R.S., Regulatory aspects on nanomedicines. Biochemical and biophysical research communications, 2015;468(3):504-510. https://doi.org/10.1016/j.bbrc.2015.08.023 PMid:26260323
20. Agrahari, V. and Agrahari, V., Facilitating the translation of nanomedicines to a clinical product: challenges and opportunities, Drug Discovery Today, 2018;23(5):974-991. https://doi.org/10.1016/j.drudis.2018.01.047 PMid:29406263
21. Ghosh, S. and Webster, T.J., Metallic nanoscaffolds as osteogenic promoters: Advances, challenges and scope. Metals, 2021;11(9):1356. https://doi.org/10.3390/met11091356
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Copyright (c) 2025 Mamatha Kola , Lakshmi Durga Annam , R. Nagaraju , Mohammad Bakhatwar
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