Anti-mitotic Activity of Methanolic Extract of Thevetia peruviana fruits

  • Lakshmi Kanta Kanthal Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657
  • Suman Pattanayak Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657
  • Susmita Roy Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657
  • Pritish Kumar Panda Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657
  • Rahul Ari Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657
  • Sanchayan Mondal Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657
  • Saikat Midya Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657
  • Argho Golui Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Abstract

Thevetia peruviana plant, a member of the Apocynaceae family, has a long history in traditional medicine for treating various ailments such as amenorrhea, malaria, jaundice, hemorrhoids, constipation, headaches, and skin issues. It contains significant amounts of cardiac glycosides in both its roots and seeds, which are known for their cytotoxic effects similar to digoxin. Given its potential toxicity and widespread use, current research is focused on assessing the antimitotic properties of the methanolic extract derived from the fruits of Thevetia peruviana. In the pursuit of potential pharmaceutical findings from plants, the antimitotic properties of the extracts were investigated through the Allium cepa assay. The findings indicated a noteworthy decrease in the mitotic index of root tips compared to the control, with the degree of mitotic suppression escalating alongside the concentration of the extracts. Therefore, the study suggests that the methanol extract derived from T. peruviana fruit has notable inhibitory and mitodepressive impacts on cell division in Allium cepa L. meristems.


Keywords: Thebetia peruviana, Anti-mitotic Activity, Allium cepa assay, mitotic index

Keywords: Thebetia peruviana, Anti-mitotic Activity, Allium cepa assay, mitotic index, Thebetia peruviana, Anti-mitotic Activity, Allium cepa assay, mitotic, index

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Author Biographies

Lakshmi Kanta Kanthal, Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Suman Pattanayak, Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Susmita Roy, Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Pritish Kumar Panda, Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Rahul Ari, Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Sanchayan Mondal, Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Saikat Midya, Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Argho Golui, Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

Department of Pharmacology, Haldia Institute of Pharmacy (An Institute of ICARE), Haldia, West Bengal, India-721657

References

1. Ahmad T, Hamid AT, Sharma A, Bhardwaj U. Thevetia peruviana: A Multipurpose Medicinal Plant-A Review. Int. J. Adv. Res. 2017;5(8):486-93. https://doi.org/10.21474/IJAR01/5081
2. Ramos-Silva A, Tavares-Carreón F, Figueroa M, De la Torre-Zavala S, Gastelum-Arellanez A, Rodríguez-García A, Galán-Wong LJ, Avilés-Arnaut H. Anticancer potential of Thevetia peruviana fruit methanolic extract. BMC Complement Altern Med. 2017 May 2;17(1):241. https://doi.org/10.1186/s12906-017-1727-y PMid:28464893 PMCid:PMC5414213
3. Magpusao AN, Omolloh G, Johnson J, Gascón J, Peczuh MW, Fenteany G. Cardiac glycoside activities link Na(+)/K(+) ATPase ion-transport to breast cancer cell migration via correlative SAR. ACS Chem Biol. 2015 Feb 20;10(2):561-9. https://doi.org/10.1021/cb500665r PMid:25334087 PMCid:PMC4340362
4. Rajamanoharan, Pholtan. (2020). POISONING CASES REPORTED BY PLANT POISONS IN SRI LANKA.
5. Zamani J, Aslani A. Cardiac findings in acute yellow oleander poisoning. J Cardiovasc Dis Res. 2010 Jan;1(1):27-8. https://doi.org/10.4103/0975-3583.59982 PMid:21188087 PMCid:PMC3004167
6. Silalahi M. Thevetia peruviana (Pers.) K. Schum.: Poisonous and its potential. Magna Scientia Advanced Research and Reviews. 2022;6(1):031-7. https://doi.org/10.30574/msarr.2022.6.1.0064
7. Singh, Kirtikalpita & Agrawal, K.K. & Mishra, V. & Uddin, S.M. & Shukla, A.. (2012). A Review on Thevetia peruviana. International Research Journal of Pharmacy. 3. 74-77.
8. Schmidt M, Bastians H. Mitotic drug targets and the development of novel anti-mitotic anticancer drugs. Drug Resist Updat. 2007 Aug-Oct;10(4-5):162-81. https://doi.org/10.1016/j.drup.2007.06.003 PMid:17669681
9. Marzo I, Naval J. Antimitotic drugs in cancer chemotherapy: promises and pitfalls. Biochem Pharmacol. 2013 Sep 15;86(6):703-10. https://doi.org/10.1016/j.bcp.2013.07.010 PMid:23886991
10. Feretti D, Zerbini I, Zani C, Ceretti E, Moretti M, Monarca S. Allium cepa chromosome aberration and micronucleus tests applied to study genotoxicity of extracts from pesticide-treated vegetables and grapes. Food Addit Contam. 2007 Jun;24(6):561-72. https://doi.org/10.1080/02652030601113602 PMid:17487597
11. Khanna, Namita & Sharma, Sonia. (2013). Allium Cepa Root Chromosomal Aberration Assay: A Review. Indian Journal of Pharmaceutical and Biological Research. 1. 105-119. https://doi.org/10.30750/ijpbr.1.3.15
12. Abubakar AR, Haque M. Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. J Pharm Bioallied Sci. 2020 Jan-Mar;12(1):1-10. https://doi.org/10.4103/jpbs.JPBS_175_19 PMid:32801594 PMCid:PMC7398001
13. Gupta, P., Patel, S. In vitro antimitotic and cytotoxic potential of plant extracts: a comparative study of Mucuna pruriens, Asteracantha longifolia and Sphaeranthus indicus. Futur J Pharm Sci 6, 115 (2020). https://doi.org/10.1186/s43094-020-00137-8
14. Sandeep, W.; Omkar, D.; Harshada, M.; Aaditi, P.; Prajwal, G.; Deepak, G.; Nilesh, G.; Ajay, K.; Mohan, K. Evaluation of Phytochemical & Antimitotic Potential of Annona Reticulata Extracts by Onion Root Model. Chem. Proc. 2021, 3, 137. https://doi.org/10.3390/ecsoc-24-08296
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1.
Kanthal LK, Pattanayak S, Roy S, Panda PK, Ari R, Mondal S, Midya S, Golui A. Anti-mitotic Activity of Methanolic Extract of Thevetia peruviana fruits. JDDT [Internet]. 15May2024 [cited 17May2024];14(5):44-8. Available from: https://jddtonline.info/index.php/jddt/article/view/6531