To Carry Out Anti-Arthritic Activity of Combination of Quercetin and Curcumin Using FCA (Freund’s Complete Adjuvant) Induced Arthritis in Laboratory Rats

  • Ajay Sunil Sabale Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211 https://orcid.org/0009-0006-1703-6554
  • Anjali Sunil Sabale Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211
  • Gajendra Kale Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211
  • Arati Machale Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211 https://orcid.org/0000-0003-0494-3922
  • H. V. Kamble Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211
  • Supriya Hambir Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Abstract

To investigate anti-arthritic activity of Quercetin and Curcumin in Freund's complete adjuvant (FCA)-induced arthritis in rats. AA was induced by injecting with Freund's complete adjuvant (FCA). Rats were randomly divided into six groups with 10 mice in each group: (1) Normal group (saline), (2) Vehicle control, (3) Leflunomide (LF, 10 mg/kg), (4) Curcumin (80), (5) Quercetin (40), (6) Curcumin (80) + Quercetin (40). Male SD rats were subjected to treatment with Lut at 10 mg/kg from days 18 to 24 after immunization. Arthritic scores, tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1), paw histopathology and the proteins of P2X4 pathway were assessed at the end of the experiment. Lut reduced the severity of arthritic scores during the experimental period as compared with positive control (RA). Lut significantly suppressed TNF-α and IL-1 as compared with RA group. Histopathological examination indicated that Lut alleviated infiltration of inflammatory cells and synovial hyperplasia as well as protected joint destruction. Lut significantly suppressed inflammatory cells, and Caspase-1p10. Quercetin and Curcumin may be a potential preventive or therapeutic candidate for the treatment of inflammation and arthritis.


Keywords: Rheumatoid arthritis, anti-arthritic activity, Quercetin, Curcumin, Freund's complete adjuvant (FCA), Leflunomide, histopathology.

Keywords: Rheumatoid arthritis, anti-arthritic activity, Quercetin, Curcumin, Freund's complete adjuvant (FCA), Leflunomide, histopathology

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Author Biographies

Ajay Sunil Sabale, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Anjali Sunil Sabale, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Gajendra Kale, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Arati Machale, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

H. V. Kamble, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Supriya Hambir, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

References

1. A Ng, M L Parker, A J Parr, P K Saunders, A C Smith, K W Waldron. Physicochemical characteristics of onion (Allium cepa L.) tissues. J Agric Food Chem. 2000 Nov;48(11):5612-7. https://doi.org/10.1021/jf991206q PMid:11087527
2. Adhirajan N, Ravi kumar T, Shanmugasundram N, Babu M. in vivo and in vitro evaluation of hair growth potential of Hibiscus rosa-sinensis Linn. J Ethanopharmacol 2003; 88:235-9. https://doi.org/10.1016/S0378-8741(03)00231-9 PMid:12963149
3. Akarasereenont, P., Mitchell, J.A., Bakhle, C., Thiemermann, C., Vane,J.R.. Comparison of the induction of cyclooxygenase and nitric oxide synthase by endotoxin in endothelial cells and macrophages. European Journal of Pharmacology 1995;273, 121-128. https://doi.org/10.1016/0014-2999(94)00680-6 PMid:7537677
4. Aletaha, D. & Smolen, J. S. Diagnosis and management of rheumatoid arthritis: a review. JAMA 320, 1360-1372. https://doi.org/ 10.1001/jama.2018.13103 (2018). https://doi.org/10.1001/jama.2018.13103 PMid:30285183
5. Alexander V A, Radhakrishnan A, and Subramani P. Overviews of Biological Importance of Quercetin: A Bioactive Flavonoid. Pharmacogn Rev. 2016 Jul-Dec; 10(20): 84-89. https://doi.org/10.4103/0973-7847.194044 PMid:28082789 PMCid:PMC5214562
6. Amador-Patarroyo, M.J., Rodriguez-Rodriguez, A., Montoya-Ortiz, G., 2012. How does age at onset influence the outcome of autoimmune diseases? Autoimmune Dis 2012, 251730. https://doi.org/10.1155/2012/251730 PMid:22195277 PMCid:PMC3238350
7. American Pharmaceutical Association 46, 515-519.
8. Aydin, S., Demir, T., Ozturk, Y., Baser, K.H.C. Analgesic activity of Nepeta italica L. Phytotherapy Research 1999;13:20-23 https://doi.org/10.1002/(SICI)1099-1573(199902)13:1<20::AID-PTR380>3.0.CO;2-J
9. Badgujar LB, Ghosh P, Gaur V, Bodhankar SL, Effect of petroleum ether extract of Sphaeranthus indicus Linn. Of complete Freunds Adjuvant induced arthritis in laboratory rats. Pharmacologyonline 2009;2: 281-291
10. Balakrishnan K. V. Postharvest technology and processing of turmeric. In: Ravindran P. N, Nirmal Babu K, Sivaraman K, editors. Turmeric: The Genus Curcuma. Boca Raton, FL: CRC Press; 2007. pp. 193-256.
11. Basu K. Bharatiya Ousadhabalir Sankhipta Bhaisajya Tattwa. Calcutta: Hahnemann Publishing Company, 1946.
12. Bendele A: Animal models of rheumatoid arthritis. J Musculoskelet Neuronal Interact 2001;1:377-385.
13. Blumenthal M, Goldberg A, Brinckmann J. Herbal Medicine: Expanded Commission E Monographs. Newton, MA: Integr Med Comm; 2000. pp. 379-84.
14. Bundy R, Walker A. F, Middleton R. W, Booth J. Turmeric extract may improve irritable bowel syndromesymptomology in otherwise healthy adults: A pilot study. J Altern Complement Med. 2004;10:1015-8. https://doi.org/10.1089/acm.2004.10.1015 PMid:15673996
15. Cerhan, J.R., Saag, K.G., Linda, M.A., Mikuls, T.R., Criswell, L.A. Antioxidant micronutrients and risk of rheumatoid arthritis in a cohort of older women. Am. J. Epidemiol 2003;157:345-354. https://doi.org/10.1093/aje/kwf205 PMid:12578805
16. Chang YC, Haung KX, Haung AC, HO YC, Wang CJ. Hibiscus anthocyanins-rich extract inhibited LDL. Oxidation and oxLDL-mediated macrophages apoptosis. Food Chem Toxicol 2006; 44:1015-23. https://doi.org/10.1016/j.fct.2005.12.006 PMid:16473450
17. Chen, Z., Bozec, A., Ramming, A., Schett, G. Anti-inflammatory and immune-regulatory cytokines in rheumatoid arthritis. Nat Rev Rheumatol 2019;15(1):9-17. https://doi.org/10.1038/s41584-018-0109-2 PMid:30341437
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Sabale AS, Sabale AS, Kale G, Machale A, Kamble HV, Hambir S. To Carry Out Anti-Arthritic Activity of Combination of Quercetin and Curcumin Using FCA (Freund’s Complete Adjuvant) Induced Arthritis in Laboratory Rats. JDDT [Internet]. 15Mar.2024 [cited 19Apr.2024];14(3):80-7. Available from: https://jddtonline.info/index.php/jddt/article/view/6463