To Carry Out Anti-Arthritic Activity of Combination of Quercetin and Curcumin Using FCA (Freund’s Complete Adjuvant) Induced Arthritis in Laboratory Rats

Authors

  • Ajay Sunil Sabale Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211 https://orcid.org/0009-0006-1703-6554
  • Anjali Sunil Sabale Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211
  • Gajendra Kale Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211
  • Arati Machale Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211 https://orcid.org/0000-0003-0494-3922
  • H. V. Kamble Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211
  • Supriya Hambir Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Abstract

To investigate anti-arthritic activity of Quercetin and Curcumin in Freund's complete adjuvant (FCA)-induced arthritis in rats. AA was induced by injecting with Freund's complete adjuvant (FCA). Rats were randomly divided into six groups with 10 mice in each group: (1) Normal group (saline), (2) Vehicle control, (3) Leflunomide (LF, 10 mg/kg), (4) Curcumin (80), (5) Quercetin (40), (6) Curcumin (80) + Quercetin (40). Male SD rats were subjected to treatment with Lut at 10 mg/kg from days 18 to 24 after immunization. Arthritic scores, tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1), paw histopathology and the proteins of P2X4 pathway were assessed at the end of the experiment. Lut reduced the severity of arthritic scores during the experimental period as compared with positive control (RA). Lut significantly suppressed TNF-α and IL-1 as compared with RA group. Histopathological examination indicated that Lut alleviated infiltration of inflammatory cells and synovial hyperplasia as well as protected joint destruction. Lut significantly suppressed inflammatory cells, and Caspase-1p10. Quercetin and Curcumin may be a potential preventive or therapeutic candidate for the treatment of inflammation and arthritis.

Keywords: Rheumatoid arthritis, anti-arthritic activity, Quercetin, Curcumin, Freund's complete adjuvant (FCA), Leflunomide, histopathology.

Keywords:

Rheumatoid arthritis, anti-arthritic activity, Quercetin, Curcumin, Freund's complete adjuvant (FCA), Leflunomide, histopathology

DOI

https://doi.org/10.22270/jddt.v14i3.6463

Author Biographies

Ajay Sunil Sabale, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Anjali Sunil Sabale, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Gajendra Kale, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Arati Machale, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

H. V. Kamble, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Supriya Hambir, Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

Shri Wagheshwar Gramvikas Pratishthan's Loknete Shri Dadapatil Pharate College of Pharmacy Mandavgan Pharata, Shirur, Pune, 412211

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Published

15-03-2024
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How to Cite

1.
Sabale AS, Sabale AS, Kale G, Machale A, Kamble HV, Hambir S. To Carry Out Anti-Arthritic Activity of Combination of Quercetin and Curcumin Using FCA (Freund’s Complete Adjuvant) Induced Arthritis in Laboratory Rats. J. Drug Delivery Ther. [Internet]. 2024 Mar. 15 [cited 2024 Dec. 6];14(3):80-7. Available from: https://jddtonline.info/index.php/jddt/article/view/6463

How to Cite

1.
Sabale AS, Sabale AS, Kale G, Machale A, Kamble HV, Hambir S. To Carry Out Anti-Arthritic Activity of Combination of Quercetin and Curcumin Using FCA (Freund’s Complete Adjuvant) Induced Arthritis in Laboratory Rats. J. Drug Delivery Ther. [Internet]. 2024 Mar. 15 [cited 2024 Dec. 6];14(3):80-7. Available from: https://jddtonline.info/index.php/jddt/article/view/6463